Invitrogen

IL-21 Monoclonal Antibody (eBio3A3-N2 (3A3-N2)), eFluor™ 660, eBioscience™

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IL-21 Antibody (50-7219-42) in Flow

Intracellular staining of Th17-polarized normal human peripheral blood cells restimulated with monensin alone (left) or PMA/Ionomycin and monensin for 5 hours (right) with Anti-Human CD4 FITC (Product # 11-0049-42) and Anti-Human IL-21 eFluor® 660. Cells in the lymphocyte gate were used for analysis.

Product Image Gallery

IL-21 Antibody — Figure 6 T cell-expressed C5aR1 regulates in vitro induction of IL-21-producing CD4+ T cells. Representative flow plots for IL-21-producing CD4+ T cells and summarized data of naive B6 CD62LhiCD44loCD4+ T cells from WT and C5ar1-/- mice cultured with aCD3/CD28 (A) or allogeneic BALB/c splenic APCs (B) under Tfh cell-inducing conditions for 5 days (n = 3 per group, repeated twice with similar results). (C) Representative flow plots for IL-21-producing CD4+ T cells and summarized data of naive B6 CD62LhiCD44loCD4+ T cells from WT TEa or C5ar1-/- TEa TCR-transgenic mice cultured with (bxd)F1 APCs under Tfh cell-inducing conditions for 5 days (n = 3/group, repeated once with similar results). (D and E) Representative flow plots (D) and quantitation (E) for c-MAF expression from WT and C5ar1-/- mice cultured with aCD3/CD28 with or without C5aR1-A for 5 days (n = 4/group, repeated twice with similar results). (F-H) Representative flow cytometry plots (F) and quantification (G) of IL-21 producers and quantitation of intracellular c-MAF (H) within human CD4+ T cells stimulated for 5 days under the conditions shown, with or without human C5aR1-A. n = 3-4/group. Each experiment shown is representative of at least 3 independent experiments for each set of studies. *P < 0.05, **P < 0.01 by Student’s t test. {TM}

产品信息

50-7219-42

应用

建议稀释比

已发表文章

流式细胞分析 (Flow)

5 µL (0.125 µg)/test

查看8篇已发表文章 8篇已发表文章

产品规格

种属反应

Human

已发表种属

Human

宿主/亚型

Mouse / IgG1, kappa

分类

Monoclonal

类型

Antibody

克隆号

eBio3A3-N2 (3A3-N2)

偶联物

eFluor™ 660 eFluor™ 660 eFluor™ 660

激发/发射光谱

651/668 nm 查看光谱

形式

Liquid

浓度

5 µL/Test

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2, with 0.2% BSA

内含物

0.09% sodium azide

保存条件

4° C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

AB_10598202

产品详细信息

Description: The monoclonal antibody eBio3A3-N2 reacts with human IL-21, a potent stimulator of NK and cytolytic T cells. IL-21 is a 131 amino acid protein most closely related to IL-2 and IL-15. Expression is found in CD4+ cells upon activation. In addition to its effect on NK cells, IL-21 stimulates proliferation of B-cell stimulated by crosslinking of the CD40 antigen and stimulates the proliferation of bone marrow progenitor cells and the expression of the NK-cell marker CD56 in the presence of IL-15.

Applications Reported: This eBio3A3-N2 (3A3-N2) antibody has been reported for use in intracellular staining followed by flow cytometric analysis.

Applications Tested: This eBio3A3-N2 (3A3-N2) antibody has been pre-titrated and tested by intracellular staining and flow cytometric analysis of restimulated, Th17-polarized normal human peripheral blood cells. This can be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Staining for IL-21 has shown donor variability. stimulation times may vary depending on the donor and should be optimized. Increased staining levels have been observed on Th17-polarized normal human peripheral blood cells.

eFluor® 660 is a replacement for Alexa Fluor® 647. eFluor® 660 emits at 659 nm and is excited with the red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome.

Excitation: 633-647 nm; Emission: 668 nm; Laser: Red Laser.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

IL-21 is a 17 kDa immunomodulatory cytokine produced mainly by Natural Killer Cells (NKT), T helper (Th) 17 and T follicular helper (TFH) cells. In TFH cells, IL-21 expression leads to autocrine signaling through the IL-21 receptor (IL-21R) and STAT3, which leads to additional transcriptional activation by Bcl6. As with IFN gamma for Th1 and IL-4 for Th2 cells, IL-21 is critical for TFH cell development and effector function. IL-21 plays a role in T cell-dependent B cell differentiation into plasma cells and memory cells, stimulation of IgG production and induction of apoptotic signaling in naive B cells. In Th17 cells, IL-21 expression and autocrine feedback through STAT3, IRF4 and ROR gamma t lead to upregulation of the IL-23R, thereby preparing Th17 cells for maturation and maintenance by the inflammatory cytokine IL-23. While upregulating IRF4 and ROR gamma t, IL-21 also mediates the downregulation of Foxp3. IL-21 deficient mice are protected from developing colitis upon chemical treatment by their inability to upregulate Th17-associated molecules. In comparison, elevated levels of IL-21 are present in chemically-induced colitis mouse models.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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