Invitrogen

IL-6 Monoclonal Antibody (MP5-20F3), PE, eBioscience™

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IL-6 Antibody (12-7061-41) in Flow

Journal of virology 2023 - FIG 4 Proinflammatory activation of macrophages by ZIKV infection in the mouse brain. (A) Representative flow plots gated on microglia (upper) and macrophages (lower) to show expression levels of proinflammatory cytokines, including IL-1beta, IL-6, and TNF-alpha. (B and C) Bar graphs indicate the percentage of activated microglia (B) and macrophages (C) expressing IL-1beta (left), IL-6 (middle), and TNF-alp... View More

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产品信息

12-7061-41

应用

建议稀释比

已发表文章

流式细胞分析 (Flow)

5 µL (0.125 µg)/test

查看15篇已发表文章 15篇已发表文章

产品规格

种属反应

Mouse

已发表种属

Mouse

宿主/亚型

Rat / IgG1, kappa

分类

Monoclonal

类型

Antibody

克隆号

MP5-20F3

偶联物

PE PE PE

激发/发射光谱

565/576 nm 查看光谱

形式

Liquid

浓度

5 µL/Test

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2, with 0.2% BSA

内含物

0.09% sodium azide

保存条件

4° C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

AB_1633409

产品详细信息

Description: The MP5-20F3 antibody reacts with mouse interleukin-6 (IL-6). The MP5-20F3 antibody is a neutralizing antibody. Mouse IL-6 (also known as B Cell Stimulating Factor, BCSF, and BSF-2) is a 21-28 kDa factor produced by a variety of cell types, including monocytes, fibroblasts and endothelial cells. Upon stimulation, macrophages, T, B, mast, and glial cells, eosinophils, keratinocytes and granulocytes also secrete IL-6. This pleiotrophic cytokine influences the antigen-specific immune response, inflammatory response, and the development of neurons, and is a major mediator of the acute phase reaction.

Applications Reported: This MP5-20F3 antibody has been reported for use in intracellular staining followed by flow cytometric analysis.

Applications Tested: This MP5-20F3 antibody has been pre-titrated and tested by intracellular staining and flow cytometric analysis of stimulated peritoneal macrophages. This can be used at test size: 5 µL (0.125 µg) per test.The pre-titrated test size contains BSA and/or gelatin for protein stabilization.

Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

IL-6 is a gene that encodes a cytokine that plays a role in inflammation and the maturation of B cells. The protein produced by this gene is an endogenous pyrogen that can induce fever in people with autoimmune diseases or infections. The gene is primarily expressed at sites of acute and chronic inflammation, where it is secreted into the serum and induces a transcriptional inflammatory response through interleukin 6 receptor, alpha. The functioning of this gene is implicated in a wide variety of inflammation-associated disease states, including suspectibility to diabetes mellitus and systemic juvenile rheumatoid arthritis. Elevated levels of the encoded protein have been found in virus infections, including COVID-19 (disease caused by SARS-CoV-2). Diseases associated with IL6 include Kaposi Sarcoma and Rheumatoid Arthritis, Systemic Juvenile.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

How to use the Panel Builder

Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

Product References

Zika Virus Infection Induces Interleukin-1β-Mediated Inflammatory Responses by Macrophages in the Brain of an Adult Mouse Model.

Journal of virology

Jeong GU,Lee S,Kim DY,Lyu J,Yoon GY,Kim KD,Ku KB,Ko J,Kwon YC

Published figure using IL-6 monoclonal antibody (Product # 12-7061-41) in Flow Cytometry

Thu Jun 29 00:00:00 UTC 2023

Lin28B-high breast cancer cells promote immune suppression in the lung pre-metastatic niche via exosomes and support cancer progression.

Nature communications

Qi M,Xia Y,Wu Y,Zhang Z,Wang X,Lu L,Dai C,Song Y,Xu K,Ji W,Zhan L

Published figure using IL-6 monoclonal antibody (Product # 12-7061-41) in Flow Cytometry

Wed Feb 16 00:00:00 UTC 2022

Loss of T cell tolerance in the skin following immunopathology is linked to failed restoration of the dermal niche by recruited macrophages.

Cell reports

West HC,Davies J,Henderson S,Adegun OK,Ward S,Ferrer IR,Tye CA,Vallejo AF,Jardine L,Collin M,Polak ME,Bennett CL

12-7061-41 was used in Flow cytometry/Cell sorting to demonstrate that pathology initiates dermis-specific macrophage differentiation and show that aGVHD-primed macrophages continue to dominate the dermal compartment at the relative expense of quiescent MHCIIint cells.

Tue May 17 00:00:00 UTC 2022

TNF-Like Ligand 1 Aberrance Aggravates Nonalcoholic Steatohepatitis via M1 Macrophage Polarization.

Oxidative medicine and cellular longevity

Luo Y,Guo J,Jia W,Wu M,Yin F,Niu G,Shih DQ,Targan SR,Zhang X

Published figure using IL-6 monoclonal antibody (Product # 12-7061-41) in Flow Cytometry

Fri Mar 04 00:00:00 UTC 2022

Critical role of OX40 in drug-induced acute liver injury.

British journal of pharmacology

Zhang C,Jin H,Wang Y,Li C,Zhao X,Li Y,Shi W,Tian Y,Xu H,Tian D,Liu K,Jia J,Sun G,Zhang D

12-7061-41 was used in Flow Cytometry to show that OX40 is a key molecule that promotes both pro-inflammatory macrophage and CD4+ T-cell function, exacerbating paracetamol-induced liver injury.

Wed Jul 01 00:00:00 UTC 2020

Loss of NF-κB1 Causes Gastric Cancer with Aberrant Inflammation and Expression of Immune Checkpoint Regulators in a STAT-1-Dependent Manner.

Immunity

O'Reilly LA,Putoczki TL,Mielke LA,Low JT,Lin A,Preaudet A,Herold MJ,Yaprianto K,Tai L,Kueh A,Pacini G,Ferrero RL,Gugasyan R,Hu Y,Christie M,Wilcox S,Grumont R,Griffin MDW,O'Connor L,Smyth GK,Ernst M,Waring P,Gerondakis S,Strasser A

12-7061-41 was used in Flow Cytometry to provide mechanistic insight into how polymorphisms that attenuate NFKB1 expression predispose humans to epithelial cancers, highlighting the pro-tumorigenic activity of STAT1 and identifying targetable vulnerabilities in gastric cancer.

Tue Mar 20 00:00:00 UTC 2018

1,25-Dihydroxyvitamin D suppresses M1 macrophages and promotes M2 differentiation at bone injury sites.

JCI insight

Wasnik S,Rundle CH,Baylink DJ,Yazdi MS,Carreon EE,Xu Y,Qin X,Lau KW,Tang X

Published figure using IL-6 monoclonal antibody (Product # 12-7061-41) in Flow Cytometry

Thu Sep 06 00:00:00 UTC 2018

Renal Sodium Gradient Orchestrates a Dynamic Antibacterial Defense Zone.

Cell

Berry MR,Mathews RJ,Ferdinand JR,Jing C,Loudon KW,Wlodek E,Dennison TW,Kuper C,Neuhofer W,Clatworthy MR

12-7061-41 was used in Flow Cytometry to reveal a critical accessory role for the homeostatic function of a vital organ in optimizing tissue defense.

Thu Aug 24 00:00:00 UTC 2017

Depletion of microglia exacerbates postischemic inflammation and brain injury.

Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism

Jin WN,Shi SX,Li Z,Li M,Wood K,Gonzales RJ,Liu Q

12-7061 was used in Flow cytometry/Cell sorting to demonstrate that depletion of microglia via administration of the dual CSF1R/c-Kit inhibitor PLX3397 exacerbates neurodeficits and brain infarction.

Thu Jun 01 00:00:00 UTC 2017

Depletion of microglia exacerbates postischemic inflammation and brain injury.

Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism

Jin WN,Shi SX,Li Z,Li M,Wood K,Gonzales RJ,Liu Q

12-7061 was used in Flow cytometry/Cell sorting to demonstrate that depletion of microglia via administration of the dual CSF1R/c-Kit inhibitor PLX3397 exacerbates neurodeficits and brain infarction.

Thu Jun 01 00:00:00 UTC 2017

Intestinal barrier dysfunction develops at the onset of experimental autoimmune encephalomyelitis, and can be induced by adoptive transfer of auto-reactive T cells.

PloS one

Nouri M,Bredberg A,Weström B,Lavasani S

12-7061 was used in Flow cytometry/Cell sorting to show that disruption of intestinal homeostasis is an early and immune-mediated event in experimental autoimmune encephalomyelitis.

Thu May 14 00:00:00 UTC 2015

Tumor suppressor p53 inhibits systemic autoimmune diseases by inducing regulatory T cells.

Journal of immunology (Baltimore, Md. : 1950)

Kawashima H,Takatori H,Suzuki K,Iwata A,Yokota M,Suto A,Minamino T,Hirose K,Nakajima H

12-7061 was used in Flow cytometry/Cell sorting to investigate how p53 suppresses autoimmunity.

Tue Oct 01 00:00:00 UTC 2013

Parvovirus evades interferon-dependent viral control in primary mouse embryonic fibroblasts.

Virology

Mattei LM,Cotmore SF,Tattersall P,Iwasaki A

12-7061 was used in Flow cytometry/Cell sorting to suggest that minute virus of mice efficiently evades antiviral immune mechanisms of type I IFNs, explaining their efficient transmission between mice.

Sat Jul 20 00:00:00 UTC 2013

CD8 regulates T regulatory cell production of IL-6 and maintains their suppressive phenotype in allergic lung disease.

Journal of immunology (Baltimore, Md. : 1950)

Joetham A,Okamoto M,Takeda K,Schedel M,Ohnishi H,Dakhama A,Gelfand EW

12-7061 was used in Flow cytometry/Cell sorting to identify the critical role of CD8-T regulatory cell interactions in regulating the suppressive phenotype of nTregs through control of IL-6 production.

Sat Jan 01 00:00:00 UTC 2011

Mechanisms by which chronic ethanol feeding limits the ability of dendritic cells to stimulate T-cell proliferation.

Alcoholism, clinical and experimental research

Fan J,Edsen-Moore MR,Turner LE,Cook RT,Legge KL,Waldschmidt TJ,Schlueter AJ

12-7061 was used in Flow cytometry/Cell sorting to assess the functional capability of ethanol-exposed dendritic cells, using a murine model of chronic alcohol abuse.

Sat Jan 01 00:00:00 UTC 2011

Mast cells counteract regulatory T-cell suppression through interleukin-6 and OX40/OX40L axis toward Th17-cell differentiation.

Blood

Piconese S,Gri G,Tripodo C,Musio S,Gorzanelli A,Frossi B,Pedotti R,Pucillo CE,Colombo MP

12-7061 was used in Flow cytometry/Cell sorting to show that mast cells, an early source of inflammatory mediators, are able to counteract Treg inhibition over effector T cells.