Invitrogen

IL-6 Monoclonal Antibody (MQ2-39C3), Biotin, eBioscience™

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IL-6 Antibody (13-7068-81) in ELISA

Frontiers in cellular and infection microbiology 2021 - Figure 4 Host response to P. aeruginosa LESB strains in vitro . (A) Human bronchial epithelial cells (HBE) were infected with P. aeruginosa LESB58 or LESB65 at a multiplicity of infection (MOI) of 1, 5, 10, or 100 for 18 h. Amount of LDH in host-cell supernatants was quantified for determination of cytotoxicity. (B) At MOI = 10, P. aeruginosa LESB65 took 48 h to elicit cytotoxicity similar to that caused in... View More

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IL-6 Antibody — FIGURE 5. TLR blocking experiments. 200,000 human PBMC cells were stimulated with GMMA from different strains. After a 4-h incubation, IL-6 release was measured by ELISA and plotted as average of duplicates with standard deviation. A, IL-6 release after stimulation with 0.0001-1000 ng/ml (10-fold steps) of GMMA from different strains. The rectangle highlights the concentration of GMMA used in blocking experiments. B-E, cells were incubated with 25 mug/ml anti-TLR4 ( dark red graphs ), 15 mug/ml anti-TLR2 ( green graphs ), or both ( violet graphs ) for 30 min before exposure to 1 or 10 ng of GMMA from Ss -p - OAg Delta msbB ( B ); Sf 2a -p - OAg Delta msbB ( C ); Ss -p - OAg Delta htrB ( D ); and Sf 2a -p - OAg Delta htrB ( E ). The graphs from experiments without blocking are shown in the same color as in A . A representative result of three independent experiments is shown. {TM}

产品信息

13-7068-81

应用

建议稀释比

已发表文章

酶联免疫吸附实验 (ELISA)

0.5-2 µg/mL

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肽阵列 (Array)

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其他PubMed (Misc)

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产品规格

种属反应

Human

已发表种属

Human, Mouse

宿主/亚型

Rat / IgG2a, kappa

分类

Monoclonal

类型

Antibody

克隆号

MQ2-39C3

偶联物

Biotin Biotin Biotin

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2

内含物

0.09% sodium azide

保存条件

4° C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

AB_466912

产品详细信息

Description: The MQ2-39C3 antibody reacts with human interleukin-6 (IL-6), a 21-28 kDa cytokine secreted by a variety of cell types.

Applications Reported: MQ2-39C3 has been reported for use in detection of human IL-6 by ELISA and immunoblotting (WB).

Applications Tested: The MQ2-39C3 antibody has been tested as the detection antibody in a sandwich ELISA for analysis of human Interleukin-6 (IL-6) in combination with the purified MQ2-13A5 (14-7069) antibody for capture and recombinant human IL-6 (14-8069) as the standard. A suitable range of concentrations of this antibody for ELISA detection is 0.5-2 µg/mL. A standard curve consisting of doubling dilutions of the recombinant standard over the range of 1000 pg/mL - 8 pg/mL should be included in each ELISA plate.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

IL-6 is a gene that encodes a cytokine that plays a role in inflammation and the maturation of B cells. The protein produced by this gene is an endogenous pyrogen that can induce fever in people with autoimmune diseases or infections. The gene is primarily expressed at sites of acute and chronic inflammation, where it is secreted into the serum and induces a transcriptional inflammatory response through interleukin 6 receptor, alpha. The functioning of this gene is implicated in a wide variety of inflammation-associated disease states, including suspectibility to diabetes mellitus and systemic juvenile rheumatoid arthritis. Elevated levels of the encoded protein have been found in virus infections, including COVID-19 (disease caused by SARS-CoV-2). Diseases associated with IL6 include Kaposi Sarcoma and Rheumatoid Arthritis, Systemic Juvenile.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Murine Model of Sinusitis Infection for Screening Antimicrobial and Immunomodulatory Therapies.

Frontiers in cellular and infection microbiology

Alford MA,Choi KG,Trimble MJ,Masoudi H,Kalsi P,Pletzer D,Hancock REW

13-7068 was used in an ELISA assay to describe a simple less virulent, epidemic P. aeruginosa cystic fibrosis clinical isolate LESB65 model allowing non-invasive tracking of bacterial infection, and screening of host-responses to infection and therapies.

Fri Jul 02 00:00:00 UTC 2021

Murine Model of Sinusitis Infection for Screening Antimicrobial and Immunomodulatory Therapies.

Frontiers in cellular and infection microbiology

Alford MA,Choi KG,Trimble MJ,Masoudi H,Kalsi P,Pletzer D,Hancock REW

Fri Jul 02 00:00:00 UTC 2021

TREM2 Alzheimer's variant R47H causes similar transcriptional dysregulation to knockout, yet only subtle functional phenotypes in human iPSC-derived macrophages.

Alzheimer's research & therapy

Hall-Roberts H,Agarwal D,Obst J,Smith TB,Monzón-Sandoval J,Di Daniel E,Webber C,James WS,Mead E,Davis JB,Cowley SA

13-7068 was used in an ELISA assay to investigated cell phenotypes of the R47H mutant and TREM2 KO in a model of human microglia, and compared their transcriptional signatures, to determine the mechanism by which R47H TREM2 disrupts function.

Mon Nov 16 00:00:00 UTC 2020

Aggregation and Its Influence on the Immunomodulatory Activity of Synthetic Innate Defense Regulator Peptides.

Cell chemical biology

Haney EF,Wu BC,Lee K,Hilchie AL,Hancock REW

13-7068-81 was used in ELISA to examine the effect of aggregation on the immunomodulatory activity of a synthetic innate defense regulator peptide, 1018 (VRLIVAVRIWRR-NH2).

Thu Aug 17 00:00:00 UTC 2017

Multiplatform Biomarker Discovery for Bladder Cancer Recurrence Diagnosis.

Disease markers

De Paoli M,Gogalic S,Sauer U,Preininger C,Pandha H,Simpson G,Horvath A,Marquette C

13-7068 was used in Antibody array to select a multiparameter panel, comprising urinary biomarkers and clinical parameters, for nonmuscle invasive bladder cancer recurrence diagnosis.

Wed Nov 20 00:00:00 UTC 2019

Toll-Like Receptor Activation by Generalized Modules for Membrane Antigens from Lipid A Mutants of Salmonella enterica Serovars Typhimurium and Enteritidis.

Clinical and vaccine immunology : CVI

Rossi O,Caboni M,Negrea A,Necchi F,Alfini R,Micoli F,Saul A,MacLennan CA,Rondini S,Gerke C

13-7068 was used in an ELISA assay to investigate the differences between Salmonella enterica strains in terms of TLR activation by generalised modules for membrane antigens from lipid A mutants.

Fri Apr 01 00:00:00 UTC 2016

Development and validation of panoptic Meso scale discovery assay to quantify total systemic interleukin-6.

British journal of clinical pharmacology

Chaturvedi S,Siegel D,Wagner CL,Park J,van de Velde H,Vermeulen J,Fung MC,Reddy M,Hall B,Sasser K

Published figure using IL-6 monoclonal antibody (Product # 13-7068-81) in ELISA

Thu Oct 01 00:00:00 UTC 2015

A microfluidic platform for high-throughput multiplexed protein quantitation.

PloS one

Volpetti F,Garcia-Cordero J,Maerkl SJ

13-7068 was used in Antibody array to test a novel device for quantifying biomarkers in multiple samples by immunoassay.

Fri Apr 29 00:00:00 UTC 2016

Modulation of endotoxicity of Shigella generalized modules for membrane antigens (GMMA) by genetic lipid A modifications: relative activation of TLR4 and TLR2 pathways in different mutants.

The Journal of biological chemistry

Rossi O,Pesce I,Giannelli C,Aprea S,Caboni M,Citiulo F,Valentini S,Ferlenghi I,MacLennan CA,D'Oro U,Saul A,Gerke C

13-7068 was used in an ELISA assay to investigate the use of gram-negative bacteria as vaccine candidates.

Fri Sep 05 00:00:00 UTC 2014

Induction of cytokines in a macrophage cell line by proteins of Clostridium difficile.

FEMS immunology and medical microbiology

Vohra P,Poxton IR

13-7068 was used in an ELISA assay to assess the induction of cytokines in a macrophage cell line in response to different antigens prepared from five Clostridium difficile strains.

Fri Jun 01 00:00:00 UTC 2012

生物信息学

蛋白别名: B-cell differentiation factor; B-cell stimulatory factor 2; BSF-2; CDF; CTL differentiation factor; H-IL-6; Hybridoma growth factor; IFN-beta-2; IL-6; ILN; Interferon beta-2; Interleukin; interleukin BSF-2; Interleukin-6; Interleukin6; M-IL-6; R-IL-6

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基因别名: BSF-2; BSF2; CDF; HGF; HSF; IFN-beta-2; IFNB2; IL-6; IL6

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UniProt ID: (Human) P05231

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Entrez Gene ID: (Human) 3569

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功能

cytokine activity interleukin-6 receptor binding protein binding growth factor activity

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