Invitrogen

IL-8 (CXCL8) Polyclonal Antibody, PeproTech®

IL-8 (CXCL8) Antibody in Western Blot (WB)

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IL-8 (CXCL8) Antibody (500-P28-1MG) in WB

Western Blot: To detect Human IL-8 (CXCL8) by Western Blot analysis IL-8 (CXCL8) Polyclonal Antibody (Product # 500-P28-1MG) can be used at a concentration of 0.1-0.2 µg/mL. When used in conjunction with compatible secondary reagents, the detection limit for Recombinant Human IL-8 (CXCL8) is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

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产品信息

500-P28-1MG

应用

建议稀释比

已发表文章

免疫印迹 (WB)

0.1-0.2 µg/mL

查看2篇已发表文章 2篇已发表文章

酶联免疫吸附实验 (ELISA)

0.5-2.0 µg/mL

查看9篇已发表文章 9篇已发表文章

免疫沉淀 (IP)

查看1篇已发表文章 1篇已发表文章

中和实验 (Neu)

查看3篇已发表文章 3篇已发表文章

阻断反应 (BLOCK)

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体外检测 (IV)

查看7篇已发表文章 7篇已发表文章

免疫染色 (IS)

查看4篇已发表文章 4篇已发表文章

产品规格

种属反应

Human

已发表种属

Human

宿主/亚型

Rabbit

分类

Polyclonal

类型

Antibody

抗原

E.coli-derived Recombinant Human IL-8 (CXCL8) (72 a.a.)

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Lyophilized

浓度

0.1-1.0 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS

内含物

no preservative

保存条件

-20°C

运输条件

Ambient

AB_2930234

产品详细信息

AA Sequence of recombinant protein: SAKELRCQCI KTYSKPFHPK FIKELRVIES GPHCANTEII VKLSDGRELC LDPKENWVQR VVEKFLKRAE NS.

Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human IL-8 (CXCL8). Anti-Human IL-8 (CXCL8)-specific antibody was purified by affinity chromatography employing an immobilized Human IL-8 (CXCL8) matrix.

Sandwich ELISA: To detect Human IL-8 (CXCL8) by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5-2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Human IL-8 (CXCL8) (500-P28Bt) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human IL-8 (CXCL8).

Western Blot: To detect Human IL-8 (CXCL8) by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. When used in conjunction with compatible secondary reagents, the detection limit for Recombinant Human IL-8 (CXCL8) is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

靶标信息

Interleukin 8 (IL-8, CXCL8) is a 72 amino acid pro-inflammatory factor which belongs to the CXC subfamily of chemokines, and are bound by the cell surface receptors IL-8RA and IL-8RB. IL-8 functions as a chemoattractant and potent angiogenic factor. The expression and secretion of IL-8 can be induced by diverse inflammatory stimuli in many cells, including macrophages and endothelial cells. In endothelial cells, IL-8 is present in storage vesicles called Weibel-Palade bodies. IL-8, first isolated from osteosarcoma cells, contains the ELR-motif (N-terminal Glu-Leu-Arg amino acid sequence) and signals through the CXCR1 and CXCR2 receptors. Previous nomenclature for IL-8 includes neutrophil activating protein 1 (NAP-1), granulocyte chemotactic protein 1 (GCP-1), monocyte-derived neutrophil-activating peptide (MONAP) and protein 3-10C. IL-8 and ten other members of the CXC chemokine gene family form a chemokine gene cluster in a region mapped to chromosome 4q. Cancer studies have demonstrated a role for IL-8 in the angiogenesis and growth of tumours, and IL-8 is believed to play a role in the pathogenesis of bronchiolitis, a common respiratory tract disease caused by viral infection.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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500-P28 was used in Enzyme-linked immunosorbent assay to investigate the role of WT-PD-L1 in triple-negative breast cancer.

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Liubomirski Y,Lerrer S,Meshel T,Rubinstein-Achiasaf L,Morein D,Wiemann S,Körner C,Ben-Baruch A

500-P28 was used in Enzyme-linked immunosorbent assay to study the impact of tumor-stroma-inflammation networks on pro-metastatic phenotypes in triple-negative breast cancer (TNBC) to identify a novel tumor-stroma-inflammation networks that may promote TNBC aggressiveness by increasing the pro-malignancy potential of the TME and of the tumor cells themselves, and reveal key roles for CXCL8 in mediating these metastasis-promoting activities.

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500-P28 was used in Enzyme-linked immunosorbent assay to investigate pro-metastatic chemokines in triple-negative breast cancer.

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Glioblastoma cell-secreted interleukin-8 induces brain endothelial cell permeability via CXCR2.

PloS one

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Lu B,Yadav S,Shah PG,Liu T,Tian B,Pukszta S,Villaluna N,Kutejová E,Newlon CS,Santos JH,Suzuki CK

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Chiu WK,Fann M,Weng NP

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Generation and growth of CD28nullCD8+ memory T cells mediated by IL-15 and its induced cytokines.

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生物信息学

蛋白别名: alveolar macrophage chemotactic factor I; ANX7; beta endothelial cell-derived neutrophil activating peptide; beta-thromboglobulin-like protein; C-X-C motif chemokine 8; Chemokine (C-X-C motif) ligand 8; Emoctakin; GCP-1; Granulocyte chemotactic protein 1; IL-8; ILN; Interleukin; interleukin 8; Interleukin-8; Interleukin8; lung giant cell carcinoma-derived chemotactic protein; lymphocyte derived neutrophil activating peptide; MDNCF; MONAP; Monocyte-derived neutrophil chemotactic factor; Monocyte-derived neutrophil-activating peptide; NAP-1; neutrophil-activating peptide 1; Neutrophil-activating protein 1; Protein 3-10C; RP11-537A6.8; small inducible cytokine subfamily B, member 8; SNX; SYNEXIN; T-cell chemotactic factor; tumor necrosis factor-induced gene 1

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基因别名: CXCL8; GCP-1; GCP1; IL8; LECT; LUCT; LYNAP; MDNCF; MONAP; NAF; NAP-1; NAP1

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UniProt ID: (Human) P10145

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Entrez Gene ID: (Human) 3576

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功能

interleukin-8 receptor binding protein binding chemokine activity chemokine

参与通路