Invitrogen

JAM3 Polyclonal Antibody

JAM3 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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JAM3 Antibody (40-8900) in IHC (P)

Immunohistochemistry analysis of JAM-C showing staining in the membrane of paraffin-embedded human kidney tissue (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, was... View More

JAM3 Antibody in Immunohistochemistry (IHC)

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产品信息

40-8900

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1-2 µg/mL

查看4篇已发表文章 4篇已发表文章

免疫组化 (IHC)

查看1篇已发表文章 1篇已发表文章

免疫组化（石蜡） (IHC (P))

1:10-1:100

产品规格

种属反应

Human, Mouse

已发表种属

Human, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Synthetic peptide derived from the C-terminal region of the mouse and rat JAM-C proteins, which differ from human by three amino acid replacements

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.25 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS, pH 7.4

内含物

0.1% sodium azide

保存条件

-20°C

运输条件

Ambient (domestic); Wet ice (international)

AB_2533485

靶标信息

JAM3 is involved in tight junctions and represent one mode of cell-to-cell adhesion in epithelial or endothelial cell sheets, forming continuous seals around cells and serving as a physical barrier to prevent solutes and water from passing freely through the paracellular space. The protein encoded by this immunoglobulin superfamily gene member is localized in the tight junctions between high endothelial cells. Unlike other proteins in this family, the this protein is unable to adhere to leukocyte cell lines and only forms weak homotypic interactions. The encoded protein is a member of the junctional adhesion molecule protein family and acts as a receptor for another member of this family.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Breast cancer resistance protein regulates apical ectoplasmic specialization dynamics stage specifically in the rat testis.

American journal of physiology. Endocrinology and metabolism

Qian X,Mruk DD,Wong EW,Cheng CY

40-8900 was used in Western Blotting to study the expression and role of breast cancer resistance protein in both Sertoli and germ cells.

Mon Apr 01 00:00:00 UTC 2013

Palladin is a regulator of actin filament bundles at the ectoplasmic specialization in adult rat testes.

Endocrinology

Qian X,Mruk DD,Wong EW,Lie PP,Cheng CY

Published figure using JAM3 polyclonal antibody (Product # 40-8900) in Western Blot

Wed May 01 00:00:00 UTC 2013

Palladin is a regulator of actin filament bundles at the ectoplasmic specialization in adult rat testes.

Endocrinology

Qian X,Mruk DD,Wong EW,Lie PP,Cheng CY

Published figure using JAM3 polyclonal antibody (Product # 40-8900) in Western Blot

Wed May 01 00:00:00 UTC 2013

Rai14 (retinoic acid induced protein 14) is involved in regulating f-actin dynamics at the ectoplasmic specialization in the rat testis*.

PloS one

Qian X,Mruk DD,Cheng CY

40-8900 was used in western blot to report that Rai14 regulates actin filaments organization in Sertoli cells during the epithelial cycle

Mon Sep 23 00:00:00 UTC 2013

Evidence for cross-reactivity of JAM-C antibodies: implications for cellular localization studies.

Biology of the cell

Betanzos A,Schnoor M,Severson EA,Liang TW,Parkos CA

40-8900 was used in Western Blotting to demonstrate that a majority of available anti-human junctional adhesion molecule C antibodies cross-react with phosphorylated K8, with implications for cellular localiSation studies.

Thu Jun 04 00:00:00 UTC 2009

生物信息学

蛋白别名: JAM-2; JAM-3; JAM-C; junction cell adhesion molecule 3; junction cell adhesion molecule C; Junctional adhesion molecule 3; Junctional adhesion molecule C

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基因别名: 1110002N23Rik; JAM-2; JAM-3; JAM-C; JAM3; JAMC; Jcam3; UNQ859/PRO1868

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UniProt ID: (Human) Q9BX67, (Mouse) Q9D8B7

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Entrez Gene ID: (Human) 83700, (Mouse) 83964

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功能

integrin binding protein binding protein homodimerization activity protein heterodimerization activity

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