Invitrogen

K-Ras Recombinant Rabbit Monoclonal Antibody (11H35L14)

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K-Ras Antibody (703345) in WB

Western blot analysis was performed on whole cell extracts (30 µg lysate) of HCT 116 (Lane 1), T-47D (Lane 2), SK-BR-3 (Lane 3), Caco-2 (Lane 4), HT-29 (Lane 5), SW480 (Lane 6) and PANC-1 (Lane 7). The blot was probed with Anti-K-Ras Recombinant Rabbit Monoclonal Antibody (Product # 703345, 1:5000 dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 1:4000 dilution). A ~21 kDa band corresponding to K-Ras was observed across the cell lines test... View More

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K-Ras Antibody — Antibody specificity was demonstrated by siRNA mediated knockdown of the target protein. HCT 116 cells were transfected with K-Ras siRNA and a decrease in signal intensity was observed in Western blot application using Anti-K-Ras Recombinant Rabbit Monoclonal Antibody (Product # 703345). {KD}

产品信息

703345

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:5,000

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免疫细胞化学 (ICC/IF)

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产品规格

种属反应

Human

已发表种属

Human

宿主/亚型

Rabbit / IgG

Expression System

Expi293

分类

Recombinant Monoclonal

类型

Antibody

克隆号

11H35L14

抗原

Peptides corresponding to human K-Ras [(aa160-aa177) (aa88-105)]

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Protein A

保存液

PBS, pH 7.4

内含物

0.09% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

AB_2784575

产品详细信息

This antibody is predicted to react with Monkey, Pig, Rat.

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

靶标信息

K-RAS (GTPase KRas) belongs to the Ras oncogene family. Ras proteins bind GDP/GTP and possess intrinsic GTPase activity. It plays an important role in the regulation of cell proliferation and has a role in promoting oncogenic events by inducing transcriptional silencing of tumor suppressor genes in colorectal cancer cells in a ZNF304-dependent manner. Mutations in the gene can result in leukemia acute myelogenous, leukemia juvenile myelomonocytic, Noonan Syndrome, gastric cancer, and cardiofaciocutaneous syndrome 2.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

CRISPR/Cas9 Gene Editing of HeLa Cells to Tag Proteins with mNeonGreen.

Bio-protocol

Surve S,Sorkin A

703345 was used in Western Blotting to describe methodological approaches to label components of the EGFR-RAS-MAPK pathway, such as Grb2, KRAS, and NRAS, with the fluorescent protein mNeonGreen (mNG) using CRISPR/Cas9 gene-editing, as well as generation of homozygous single-cell clones of the edited target protein.

Fri May 20 00:00:00 UTC 2022

EGFR-RAS-MAPK signaling is confined to the plasma membrane and associated endorecycling protrusions.

The Journal of cell biology

Surve S,Watkins SC,Sorkin A

703345 was used in Immunocytochemistry-immunofluorescence to propose that a small pool of surface EGFRs sustain signaling within the RAS-ERK1/2 pathway and that RAS activation persists in TERCs, whereas endosomal EGFR does not significantly contribute to ERK1/2 activity.

Mon Nov 01 00:00:00 UTC 2021

KARATE: PKA-induced KRAS4B-RHOA-mTORC2 supercomplex phosphorylates AKT in insulin signaling and glucose homeostasis.

Molecular cell

Senoo H,Murata D,Wai M,Arai K,Iwata W,Sesaki H,Iijima M

703345 was used in Western Blotting to reveal a fundamental mechanism that activates mTORC2 toward AKT in insulin-regulated glucose homeostasis.

Thu Nov 18 00:00:00 UTC 2021

生物信息学

蛋白别名: c-K-ras; c-Ki-ras; c-Kirsten-ras protein; cellular c-Ki-ras2; cellular c-Ki-ras2 proto-oncogene; GTPase KRas; K RAS; K-Ras 2; K-ras p21 protein; Ki-Ras; Kirsten rat sarcoma viral oncogene homolog; oncogene KRAS2; PR310 c-K-ras oncogene; RASK; transforming protein p21; v-Ki-ras2 Kirsten rat sarcoma 2 viral oncogene homolog

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基因别名: C-K-RAS; CFC2; K-RAS2A; K-RAS2B; K-RAS4A; K-RAS4B; KI-RAS; KRAS; KRAS1; KRAS2; NS; NS3; RALD; RASK2

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UniProt ID: (Human) P01116

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Entrez Gene ID: (Human) 3845

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功能

GTPase activity protein binding GTP binding GMP binding GDP binding LRR domain binding protein complex binding small GTPase

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