Invitrogen

KIAA1456 Polyclonal Antibody

2个已发表图片

1 篇参考文献

查看其他5个KIAA1456抗体

抗体检测数据 (1)
已发表图片 (2)

KIAA1456 Antibody in Western Blot (WB)

KIAA1456 Antibody in Western Blot (WB)

图: 1 / 3

KIAA1456 Antibody (PA5-77162) in WB

Western blot analysis of KIAA1456 in extracts of various cell lines. Samples were incubated with KIAA1456 polyclonal antibody (Product # PA5-77162).

KIAA1456 Antibody in Western Blot (WB)

KIAA1456 Antibody in Western Blot (WB)

KIAA1456 Antibody in Western Blot (WB)

Product Image Gallery

KIAA1456 Antibody in Western Blot (WB) — Western blot analysis of KIAA1456 in extracts of various cell lines. Samples were incubated with KIAA1456 polyclonal antibody (Product # PA5-77162).

KIAA1456 Antibody in Western Blot (WB) — Effect of KIAA1456 overexpression and depletion on mcm 5 U and mcm 5 s 2 U modification levels and on CHIKV infection. a Western blot showing KIAA1456 protein upregulation upon CHIKV infection. Values denote relative KIAA1456 expression normalized by tubulin expression calculated from 3 independent replicates and expressed as mean +- SEM. b Western blot showing KIAA1456 protein levels under plasmid-driven overexpression (KIAA1456-OE) and CHIKV infection conditions. Values represent relative KIAA1456 expression normalized by vinculin expression. A representative example from two independent replicates is shown. Note, that to be able to compare expression levels, 1:5 dilutions of the KIAA1456-OE overexpression samples were loaded. c Relative mcm 5 U and mcm 5 s 2 U modification levels in mock-infected cells, CHIKV-infected cells and in cells overexpressing KIAA1456 (KIAA1456-OE) determined by mass LC-MS-MS; bars represent the mean value +- SEM of 4 (in the case of mcm 5 after KIAA1456-OE) respectively 5 (in the remaining cases) independent replicates. p -values: mcm 5 - mock vs CHIKV: 0.011, mcm5 - mock vs KIAA-OE: 0.016. d Relative amount of CHIKV RNA levels ( p -value 0.0046) and ( e ) of CHIKV capsid protein levels in CHIKV-infected cells transfected with an empty plasmid or with a plasmid overexpressing KIAA1456 (KIAA1456-OE) determined by qPCR; bars represent the mean value +- SEM of 5 (empty) and 4 (KIAA-OE) independent replicates. CHIKV RNA and capsid protein expression

KIAA1456 Antibody in Western Blot (WB) — Effect of DENV and HCV infection on KIAA1456 protein levels and mcm 5 and mcm 5 s 2 U levels. a Western blot showing KIAA1456 protein expression levels upon DENV and HCV infection. Values denote KIAA1456 expression normalized by tubulin expression relative to uninfected cells calculated from 3 independent replicates and expressed as mean +- SEM. b as in Fig. 5c but upon DENV and HCV infection. Bars represent the mean value +- SEM of 5 independent replicates ( p -value 0.007). Statistical analyses were performed using the two-tailed t -test. * p < 0.05.

产品信息

PA5-77162

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:2,000

查看1篇已发表文章 1篇已发表文章

产品规格

种属反应

Human, Mouse, Rat

已发表种属

Not Applicable

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Recombinant full length Human KIAA1456.

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS, pH 7.2, with 50% glycerol

内含物

0.02% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

AB_2720889

产品详细信息

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

靶标信息

C8orf79 (chromosome 8 open reading frame 79) is a 454 amino acid protein that belongs to the methyltransferase superfamily and is encoded by a gene that maps to human chromosome 8p22. Consisting of nearly 146 million bases, chromosome 8 encodes about 800 genes. Translocation of portions of chromosome 8 with amplifications of the c-Myc gene are found in some leukemias and lymphomas, and are typically associated with a poor prognosis. Portions of chromosome 8 have been linked to schizophrenia and bipolar disorder.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

CHIKV infection reprograms codon optimality to favor viral RNA translation by altering the tRNA epitranscriptome.

Nature communications

Jungfleisch J,Böttcher R,Talló-Parra M,Pérez-Vilaró G,Merits A,Novoa EM,Díez J

Published figure using KIAA1456 polyclonal antibody (Product # PA5-77162) in Western Blot

Thu Aug 11 00:00:00 UTC 2022

生物信息学

蛋白别名: Probable tRNA methyltransferase 9-like protein; Probable tRNA methyltransferase 9B; RM9L

查看更多收起

基因别名: C8orf79; KIAA1456; TRM9L; TRMT9B; Trmt9l

查看更多收起

UniProt ID: (Mouse) Q80WQ4, (Human) Q9P272

查看更多收起

Entrez Gene ID: (Mouse) 319582, (Human) 57604

查看更多收起

rRNA (adenine-N6,N6-)-dimethyltransferase activity molecular_function protein C-terminal carboxyl O-methyltransferase activity tRNA (guanine-N2-)-methyltransferase activity methyltransferase activity C-methyltransferase activity N-methyltransferase activity O-methyltransferase activity S-methyltransferase activity RNA methyltransferase activity mRNA methyltransferase activity tRNA methyltransferase activity protein methyltransferase activity 2-polyprenyl-6-methoxy-1,4-benzoquinone methyltransferase activity rRNA methyltransferase activity rRNA (uridine-2'-O-)-methyltransferase activity rRNA (adenine-N6-)-methyltransferase activity DNA-methyltransferase activity tRNA (guanine-N1-)-methyltransferase activity rRNA (cytosine-C5-)-methyltransferase activity selenocysteine methyltransferase activity arginine N-methyltransferase activity protein-arginine N-methyltransferase activity lysine N-methyltransferase activity protein-lysine N-methyltransferase activity tRNA (uracil) methyltransferase activity tRNA (guanine) methyltransferase activity tRNA (adenine) methyltransferase activity tRNA (cytosine) methyltransferase activity tRNA (cytosine-5-)-methyltransferase activity tRNA (adenine-N1-)-methyltransferase activity rRNA (adenine) methyltransferase activity rRNA (cytosine) methyltransferase activity rRNA (guanine) methyltransferase activity rRNA (uridine) methyltransferase activity transferase activity protein C-terminal leucine carboxyl O-methyltransferase activity 1-phenanthrol methyltransferase activity protein-arginine N5-methyltransferase activity methylarsonite methyltransferase activity dimethylarsinite methyltransferase activity 4,5-dihydroxybenzo(a)pyrene methyltransferase activity 1-hydroxypyrene methyltransferase activity 1-hydroxy-6-methoxypyrene methyltransferase activity demethylmenaquinone methyltransferase activity cobalt-precorrin-6B C5-methyltransferase activity cobalt-precorrin-7 C15-methyltransferase activity cobalt-precorrin-5B C1-methyltransferase activity cobalt-precorrin-3 C17-methyltransferase activity dimethylamine methyltransferase activity hydroxyneurosporene-O-methyltransferase activity tRNA (adenine-57, 58-N(1)-) methyltransferase activity methylamine-specific methylcobalamin:coenzyme M methyltransferase activity trimethylamine methyltransferase activity methanol-specific methylcobalamin:coenzyme M methyltransferase activity monomethylamine methyltransferase activity P-methyltransferase activity Se-methyltransferase activity 2-phytyl-1,4-naphthoquinone methyltransferase activity tRNA (uracil-2'-O-)-methyltransferase activity tRNA (cytosine-2'-O-)-methyltransferase activity phosphomethylethanolamine N-methyltransferase activity tRNA (cytosine-3-)-methyltransferase activity rRNA (cytosine-2'-O-)-methyltransferase activity rRNA (cytosine-N4-)-methyltransferase activity trihydroxyferuloyl spermidine O-methyltransferase activity tRNA binding ferrous iron binding oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, 2-oxoglutarate as one donor, and incorporation of one atom each of oxygen into both donors

tRNA wobble uridine modification tRNA modification tRNA methylation oxidation-reduction process tRNA processing biological_process metabolic process methylation