Invitrogen

KIF23 Polyclonal Antibody

KIF23 Antibody in Immunocytochemistry (ICC/IF)

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KIF23 Antibody (PA5-31773) in ICC/IF

KIF23 Polyclonal Antibody detects MKLP1 protein at midbody by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: MKLP1 protein stained by KIF23 Polyclonal Antibody (Product # PA5-31773) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 µm.

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KIF23 Antibody — Inhibition of KIF23 blocked the function of KIF23 knockdown in PCa cells. 22RV1 and DU145 cells were introduced with si-KIF23, si-NC, si-KIF23+miR-15a-5p inhibitor and si-KIF23+inhibitor NC, respectively. a, b The expression of KIF23, c cell proliferation, d cell apoptosis, e cell migration (100), f cell invasion (100) and g the levels of Vimentin, E-cad, Caspase-3 and CyclinD1 were detected. *P<0.05, **P<0.01 {KD}

产品信息

PA5-31773

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:3,000

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免疫细胞化学 (ICC/IF)

1:100-1:1,000

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产品规格

种属反应

Human

已发表种属

Human

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Recombinant fragment corresponding to a region within amino acids 1 and 214 of Human MKLP1

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.8 mg/mL

纯化类型

Antigen affinity chromatography

保存液

0.1M tris glycine, pH 7, with 20% glycerol

内含物

0.01% thimerosal

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

AB_2549246

产品详细信息

Recommended positive controls: HeLa.

Predicted reactivity: Mouse (91%), Rat (90%), Bovine (93%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

靶标信息

The protein encoded by this gene is a member of kinesin-like protein family. This family includes microtubule-dependent molecular motors that transport organelles within cells and move chromosomes during cell division. This protein has been shown to cross-bridge antiparallel microtubules and drive microtubule movement in vitro. Alternate splicing of this gene results in two transcript variants encoding two different isoforms.

⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Rab14/MACF2 complex regulates endosomal targeting during cytokinesis.

Molecular biology of the cell

Gibieža P,Peterman E,Hoffman HK,Van Engeleburg S,Skeberdis VA,Prekeris R

PA5-31773 was used in Immunocytochemistry to demonstrate that depletion of Rab14 causes either cytokinesis failure or significantly prolongs division time.