Invitrogen

KPNA4 Polyclonal Antibody

KPNA4 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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KPNA4 Antibody (PA5-18239) in IHC (P)

Immunohistochemistry analysis of KPNA4 in human small intestine. Samples were incubated with KPNA4 polyclonal antibody (Product # PA5-18239) using a dilution of 5 µg/mL. Formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

KPNA4 Antibody in Immunocytochemistry (ICC/IF)

Product Image Gallery

KPNA4 Antibody — Phosphorylation of KPNA4 at S60 determines KPNA4 transport activity. a Schematic representation of the structural and functional domains and phosphorylation site of KPNA4 (upper panel), and evolutionarily conserved IBB domain in KPNA4 protein (bottom panel). b-d SAS stably expressing KPNA4-tag was subjected to IP analysis under MAPK inhibition. At 24 h treatment of U0126 (10 µM), cells were harvested, lysed, and proceeded for pull-down assay using antiphospho-Ser antibody (b) or His-tag pull-down assay (c). Quantification of the interaction between KPNB1 and KPNA4 (d). Relative interacting amounts was normalized by KPNA4 levels, then amounts of KPNB1 without U0126 was considered as 100% (n = 3). One sample t test was performed using GraphPad QuickCalcs. **p < 0.01. e Diagram of mutation conducted in KPNA4. f Interactions of KPNA4 and RREB1 were addressed by western blot analysis. At 48 h after transfection, cells were harvested, lysed, and proceeded for His-tag pull-down assay. g Quantification of the interaction between KPNA4 and RREB1. Relative interacting amounts were normalized by KPNA4 levels, then amounts of RREB1 interacting KPNA4S60A was considered as 100% (n=3). One sample t test was performed using GraphPad QuickCalcs. *p<0.05. h Western blot analysis of exogenous KPNA4S60A or KPNA4S60D. i Short-term proliferation ability was analyzed MTT assay using HNSCCs expressing either KPNA4S60A or KPNA4S60D under shKPNA4 expression. Data represent means ±SD (n=3). One sample t test was performed using GraphPad QuickCalcs. *p< 0.05. {KD}

产品信息

PA5-18239

应用

建议稀释比

已发表文章

免疫印迹 (WB)

0.03-0.3 µg/mL

免疫组化 (IHC)

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免疫组化（石蜡） (IHC (P))

Assay-dependent

免疫细胞化学 (ICC/IF)

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产品规格

种属反应

Human, Mouse, Rat

已发表种属

Human

宿主/亚型

Goat / IgG

分类

Polyclonal

类型

Antibody

抗原

Synthetic peptide sequence (NSSANVPTEGFQF) corresponding to the C-terminus amino acids of KPNA4 (aa 509-521).

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Ammonium sulfate precipitation

保存液

TBS, pH 7.3, with 0.5% BSA

内含物

0.02% sodium azide

保存条件

-20° C, Avoid Freeze/Thaw Cycles

运输条件

Wet ice

AB_10979728

产品详细信息

This antibody is predicted to react with bovine, canine and rat based on sequence homology.

This antibody is tested in Peptide ELISA: antibody detection limit dilution 128,000.

靶标信息

The nuclear import of karyophilic proteins is directed by short amino acid sequences termed nuclear localization signals (NLSs). Karyopherins, or importins, are cytoplasmic proteins that recognize NLSs and dock NLS-containing proteins to the nuclear pore complex. The protein encoded by this gene shares the sequence similarity with Xenopus importin-alpha and Saccharomyces cerevisiae Srp1. This protein is found to interact with the NLSs of DNA helicase Q1 and SV40 T antigen.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Disease-specific alteration of karyopherin-α subtype establishes feed-forward oncogenic signaling in head and neck squamous cell carcinoma.

Oncogene

Hazawa M,Sakai K,Kobayashi A,Yoshino H,Iga Y,Iwashima Y,Lim KS,Chih-Cheng Voon D,Jiang YY,Horike SI,Lin DC,Wong RW

PA5-18239 was used in Immunohistochemistry to report that KPNA4, encoding karyopherin-α4 (KPNA4), is exclusively amplified and overexpressed in head and neck of squamous cell carcinoma (HNSCC).

Sun Mar 01 00:00:00 UTC 2020

Disease-specific alteration of karyopherin-α subtype establishes feed-forward oncogenic signaling in head and neck squamous cell carcinoma.

Oncogene

Hazawa M,Sakai K,Kobayashi A,Yoshino H,Iga Y,Iwashima Y,Lim KS,Chih-Cheng Voon D,Jiang YY,Horike SI,Lin DC,Wong RW

PA5-18239 was used in Immunohistochemistry to report that KPNA4, encoding karyopherin-α4 (KPNA4), is exclusively amplified and overexpressed in head and neck of squamous cell carcinoma (HNSCC).

Sun Mar 01 00:00:00 UTC 2020

生物信息学

蛋白别名: Importin alpha Q1; Importin subunit alpha-3; importin subunit alpha-4; importin-alpha-Q1; karyopherin (importin) alpha 4; karyopherin alpha 4; karyopherin alpha 4 (importin alpha 3); Karyopherin subunit alpha-4; MGC12217; MGC26703; predicted role in nuclear import; Qip1; Qip1 protein

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基因别名: 1110058D08Rik; importin; IPOA3; KPNA4; QIP1; SRP3

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UniProt ID: (Human) O00629, (Mouse) O35343

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Entrez Gene ID: (Human) 3840, (Mouse) 16649, (Rat) 361959

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功能

protein binding nuclear localization sequence binding protein transporter activity

参与通路

protein import into nucleus transport protein transport response to hydrogen peroxide NLS-bearing protein import into nucleus modulation by virus of host process intracellular transport of virus