Invitrogen

Ly-49G2 Monoclonal Antibody (eBio4D11 (4D11)), FITC, eBioscience™

2个已发表图片

10 篇参考文献

说明书

实验方案

Questions & Answers

技术支持

说明书

实验方案

Questions & Answers

技术支持

抗体检测数据 (1)
已发表图片 (2)

Ly-49G2 Antibody in Flow Cytometry (Flow)

图: 1 / 3

Ly-49G2 Antibody (11-5781-82) in Flow

Staining of C57BL/6 splenocytes with Anti-Mouse CD49b (Integrin alpha 2) PE (Product # 12-5971-82) and 0.125 µg of Rat IgG2a kappa Isotype Control FITC (Product # 11-4321-42) (left) or 0.125 µg of Anti-Mouse Ly-49G2 FITC (right). Total viable cells were used for analysis.

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产品信息

11-5781-82

应用

建议稀释比

已发表文章

流式细胞分析 (Flow)

0.25 µg/test

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产品规格

种属反应

Mouse

已发表种属

Mouse

宿主/亚型

Rat / IgG2a, kappa

分类

Monoclonal

类型

Antibody

克隆号

eBio4D11 (4D11)

偶联物

FITC FITC FITC

激发/发射光谱

498/517 nm 查看光谱

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2

内含物

0.09% sodium azide

保存条件

4° C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

AB_763604

产品详细信息

Description: The 4D11 monoclonal antibody reacts with Ly-49G2 (also known as LGL-1). The Ly-49 family of NK-cell receptors, members of the C-type lectin superfamily, are disulfide-linked type-II transmembrane protein homodimers with extracellular carbohydrate recognition domains, which bind to MHC class I alloantigens. The Ly-49 family members are expressed independently, such that an individual NK or T cell may display more than one class of Ly-49 receptor. Ly-49G2 is an inhibitory receptor expressed on subsets of natural killer (NK) cells, NK-T cells (in all strains tested), and on a population of memory CD8+ T cells (in C57BL/6 mice). The 4D11 antibody has been reported to cross react with the Ly-49AB6, Ly-49ABALB, and Ly-49T129/J inhibitory receptors and Ly-49LCBA/J activatingreceptors. The proportion of NK-T cells expressing Ly-49A and Ly-49G2 is higher in the thymus than in liver, and there is evidence that down-regulation of Ly-49 receptor expression is necessary for normal NK-T-cell development to occur.

Applications Reported: This eBio4D11 (4D11) antibody has been reported for use in flow cytometric analysis.

Applications Tested: This eBio4D11 (4D11) antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

Mouse Ly49 receptors exhibit allelic specificity for MHC class I Ia molecules and are thought to serve to prevent natural killer (NK) cells from attacking normal cells, while allowing them to attack infected or transformed cells in which class I molecules have been down-regulated. These inhibitory receptors are also expressed on subpopulations of mouse CD8+ T cells.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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Product References

Binary outcomes of enhancer activity underlie stable random monoallelic expression.

eLife

Kissiov DU,Ethell A,Chen S,Wolf NK,Zhang C,Dang SM,Jo Y,Madsen KN,Paranjpe I,Lee AY,Chim B,Muljo SA,Raulet DH

Published figure using Ly-49G2 monoclonal antibody (Product # 11-5781-82) in Flow Cytometry

Thu May 26 00:00:00 UTC 2022

PD-1 expression on mouse intratumoral NK cells and its effects on NK cell phenotype.

iScience

Wagner AK,Kadri N,Tibbitt C,van de Ven K,Bagawath-Singh S,Oliinyk D,LeGresley E,Campbell N,Trittel S,Riese P,Ribacke U,Sandalova T,Achour A,Kärre K,Chambers BJ

11-5781-82 was used in Flow cytometry/Cell sorting to demonstrate that there may be a role for the PD-1/PD-L1 axis in tumor-infiltrating NK cells in vivo.

Fri Oct 21 00:00:00 UTC 2022

The transcriptional repressor ID2 supports natural killer cell maturation by controlling TCF1 amplitude.

The Journal of experimental medicine

Li ZY,Morman RE,Hegermiller E,Sun M,Bartom ET,Maienschein-Cline M,Sigvardsson M,Kee BL

11-5781-82 was used in Flow Cytometry to demonstrate that ID2 sets a threshold for TCF1 during NK cell development, thus controlling the balance of immature and terminally differentiated cells that support future NK cell responses.

Mon Jun 07 00:00:00 UTC 2021

The transcriptional repressor ID2 supports natural killer cell maturation by controlling TCF1 amplitude.

The Journal of experimental medicine

Li ZY,Morman RE,Hegermiller E,Sun M,Bartom ET,Maienschein-Cline M,Sigvardsson M,Kee BL

Mon Jun 07 00:00:00 UTC 2021

IFN-γ modulates Ly-49 receptors on NK cells in IFN-γ-induced pregnancy failure.

Scientific reports

Li ZY,Song ZH,Meng CY,Yang DD,Yang Y,Peng JP

11-5781 was used in Flow cytometry/Cell sorting to investigate the mechanism by which IFN-γ modulates Ly-49 receptors on natural killer cells during pregnancy failure.

Fri Dec 11 00:00:00 UTC 2015

Viral MHC class I-like molecule allows evasion of NK cell effector responses in vivo.

Journal of immunology (Baltimore, Md. : 1950)

Pyzik M,Dumaine A,Charbonneau B,Fodil-Cornu N,Jonjic S,Vidal SM

11-5781 was used in Flow cytometry/Cell sorting to show that recognition of the viral protein m157 by the inhibitory Ly49C receptor allows escape from natural killer cell control of viral replication.

Mon Dec 15 00:00:00 UTC 2014

A dynamic flux in natural killer cell subsets as a function of the duration of alcohol ingestion.

Alcoholism, clinical and experimental research

Ballas ZK,Cook RT,Shey MR,Coleman RA

11-5781 was used in Flow cytometry/Cell sorting to investigate the effect of duration of alcohol ingestion on NK cell function, showing a dynamic flux in NK cell subsets as a function of ingestion.

Tue May 01 00:00:00 UTC 2012

Distinct MHC class I-dependent NK cell-activating receptors control cytomegalovirus infection in different mouse strains.

The Journal of experimental medicine

Pyzik M,Charbonneau B,Gendron-Pontbriand EM,Babić M,Krmpotić A,Jonjić S,Vidal SM

11-5781 was used in Flow cytometry/Cell sorting to study how multiple activating Ly49 receptors may participate in H-2-dependent recognition of MCMV infection, providing a common mechanism of NK cell-mediated resistance against viral infection.

Mon May 09 00:00:00 UTC 2011

Interleukin-22 (IL-22) production by pulmonary Natural Killer cells and the potential role of IL-22 during primary influenza virus infection.

Journal of virology

Guo H,Topham DJ

11-5781 was used in Flow cytometry/Cell sorting to test whether interleukin-22 (IL-22) is protective during influenza virus infection, showing that IL-22 plays a marginal role for protection, with a differential requirement of this cytokine for bacterial and viral infections.