Invitrogen

MDA5 Recombinant Rabbit Monoclonal Antibody (33H12L34)

MDA5 Antibody in Immunocytochemistry (ICC/IF)

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MDA5 Antibody (700360) in ICC/IF

Immunofluorescence analysis of MDA5 was done on 70% confluent log phase A431 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with MDA5 (33H12L34), Recombinant Rabbit Monoclonal Antibody (Product # 700360) at 2 µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate ... View More

MDA5 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

MDA5 Antibody in Immunoprecipitation (IP)

MDA5 Antibody in RNA Immunoprecipitation (RIP)

Product Image Gallery

MDA5 Antibody — RIG-I like receptor and IFN-lambda receptor silencing promotes HCV multicycle growth in HepG2-HFL cells. (A) Immunoblots of endogenous RIG-I, MDA5, or ß-actin were performed on lysates from HepG2-HFL cells that were transduced with lentiviral vectors expressing shRNA targeting RIG-I, MDA5, or a no targeting (NT) shRNA used as a negative control. (B) IL-29 production was assayed by ELISA 24 hpi with SeV and 24 hours transfectionwith poly(I:C). (C) HCV-GLuc multicycle growth was monitored by sampling luciferase production at 2, 4, and 7 dpi. (D) IL-29 mRNA induction was assessed in HepG2-HFL cells 30 hpi with non-reporter HCV. (E) Quantification of IFN-lambda receptor (IL28RA) mRNA levels in HepG2-HFL cells transduced with two different lentiviral vectors expressing shRNA targeting IL28RA (#1 and #2), or a control shRNA (NT). (F) HCV-GLuc multicycle growth was monitored by sampling luciferase production at 2, 4, and 7 dpi. Asterisks represent statistically significant differences between NT-shRNA expressing cells and cells expressing shRNA targeting RIG-I, MDA5, or IL-28RA at the indicated time points.
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产品信息

700360

应用

建议稀释比

已发表文章

免疫印迹 (WB)

4-6 µg/mL

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免疫组化（石蜡） (IHC (P))

1:20

免疫细胞化学 (ICC/IF)

4-6 µg/mL

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流式细胞分析 (Flow)

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免疫沉淀 (IP)

Assay-dependent

RNA免疫共沉淀 (RIP)

Assay-dependent

产品规格

种属反应

Human, Mouse

已发表种属

Human, Virus

宿主/亚型

Rabbit / IgG

Expression System

Expi293

分类

Recombinant Monoclonal

类型

Antibody

克隆号

33H12L34

抗原

A recombinant protein corresponding to amino acids 883-1025 of Q9BYX4.

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Protein A

保存液

PBS

内含物

0.09% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

AB_2532316

产品详细信息

This antibody is predicted to react with Rhesus monkey, mouse, rat, porcine and bovine based on sequence homology.

Intact IgG appears on a non-reducing gel as ~150 kDa band and upon reduction generating a ~25 kDa light chain band and a ~50 kDa heavy chain.

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

靶标信息

DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp and a protein kinase C-activating compound, mezerein. Irreversible reprogramming of melanomas can be achieved by treatment with both these agents; treatment with either agent alone only achieves reversible differentiation.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

RIG-I and MDA5 are modulated by bone morphogenetic protein (BMP6) and are essential for restricting Zika virus infection in human Sertoli cells.

Frontiers in microbiology

Jiyarom B,Giannakopoulos S,Strange DP,Panova N,Gale M,Verma S

Published figure using MDA5 recombinant monoclonal antibody (Product # 700360) in Western Blot

Fri May 05 00:00:00 UTC 2023

Activation of NF-κB signaling via cytosolic mitochondrial RNA sensing in kerotocytes with mitochondrial DNA common deletion.

Scientific reports

Zhou X,Backman LJ,Danielson P

700360 was used in Western Blot to provide a novel explanation of the different clinical outcome in different regions of the cornea during wound healing.

Thu Apr 01 00:00:00 UTC 2021

Activation of NF-κB signaling via cytosolic mitochondrial RNA sensing in kerotocytes with mitochondrial DNA common deletion.

Scientific reports

Zhou X,Backman LJ,Danielson P

700360 was used in Western Blot to provide a novel explanation of the different clinical outcome in different regions of the cornea during wound healing.

Thu Apr 01 00:00:00 UTC 2021

Dietary Supplementation with Biobran/MGN-3 Increases Innate Resistance and Reduces the Incidence of Influenza-like Illnesses in Elderly Subjects: A Randomized, Double-Blind, Placebo-Controlled Pilot Clinical Trial.

Nutrients

Elsaid AF,Agrawal S,Agrawal A,Ghoneum M

700360 was used in Flow Cytometry to investigate the effect of Biobran/MGN-3 on ILI incidence, natural killer cell activity, and the expressions of RIG-1, and their downstream signaling genes ISG-15 and MX1.

Fri Nov 19 00:00:00 UTC 2021

Double-stranded RNA innate immune response activation from long-term adeno-associated virus vector transduction.

JCI insight

Shao W,Earley LF,Chai Z,Chen X,Sun J,He T,Deng M,Hirsch ML,Ting J,Samulski RJ,Li C

700360 was used in Western Blotting to study the role of the innate immune response at the later time points following adeno-associated viral vector transduction.

Thu Jun 21 00:00:00 UTC 2018

Rabies Virus Infection Induces the Formation of Stress Granules Closely Connected to the Viral Factories.

PLoS pathogens

Nikolic J,Civas A,Lama Z,Lagaudrière-Gesbert C,Blondel D

700360 was used in immunocytochemistry to report that rabies virus induces stress granule formation

Sat Oct 01 00:00:00 UTC 2016

HepG2 cells mount an effective antiviral interferon-lambda based innate immune response to hepatitis C virus infection.

Hepatology (Baltimore, Md.)

Israelow B,Narbus CM,Sourisseau M,Evans MJ

700360 was used in western blot to study the IFN-lambda inate immune response of HepG2 cells in response to HCV infection

Wed Oct 01 00:00:00 UTC 2014

生物信息学

蛋白别名: CADM-140 autoantigen; Clinically amyopathic dermatomyositis autoantigen 140 kDa; DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide; Helicard; Helicase with 2 CARD domains; Interferon induced with helicase C domain protein 1; interferon induced, with helicase C domain 1; Interferon-induced helicase C domain-containing protein 1; Interferon-induced with helicase C domain protein 1; MDA-5; melanoma differentiation associated protein-5; melanoma differentiation-associated gene 5; Melanoma differentiation-associated protein 5; MGC133047; Murabutide down-regulated protein; RIG-I-like receptor 2; RLR-2; RNA helicase-DEAD box protein 116

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基因别名: 9130009C22Rik; AGS7; Helicard; Hlcd; IDDM19; IFIH1; MDA-5; MDA5; RH116; RLR-2; SGMRT1

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UniProt ID: (Human) Q9BYX4, (Mouse) Q8R5F7

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Entrez Gene ID: (Human) 64135, (Mouse) 71586

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功能