Invitrogen

MERTK Monoclonal Antibody (DS5MMER), eBioscience™

MERTK Antibody in Immunohistochemistry (Frozen) (IHC (F))

图: 1 / 10

MERTK Antibody (14-5751-82) in IHC (F)

Immunohistochemistry of frozen mouse liver using 5 µg/mL of Rat IgG2a K Isotype Control Purified (left) or 5 µg/mL of Anti-Mouse MerTK Purified (right) followed by Anti-Rat IgG TRITC.Nuclei are counterstained with DAPI.

MERTK Antibody in Immunohistochemistry (IHC)

Product Image Gallery

MERTK Antibody — Figure 4 Role of AXL and MER in MuV replication. (A) Expression of AXL and MER receptors. SC and LC were isolated from 3-week-old wide-type (WT), Axl knockout (Axl -/-), Mer knockout (Mer -/-), and Axl/Mer double knockout (Axl -/- Mer -/-) mice. The expression of AXL and MER was examined by Western blot. (B) MuV binding. SC and LC with the indicated genotypes were incubated with 100 MOI of MuV for 1 h on ice. MuV-NP RNA (upper panels) and protein (lower panels) levels were determined by real-time qRT-PCR and Western blot, respectively. (C) MuV internalization. SC and LC were incubated with 100 MOI of MuV for 1 h at 37°C. MuV-NP RNA (upper panels) and protein (lower panels) levels were determined. (D) MuV replication. SC and LC were infected with 1.0 MOI of MuV. MuV-NP RNA (upper panels) and protein (lower panels) levels were determined at 48 h after infection. (E) Intracellular MuV. WT and Axl -/- Mer -/- SC (left panels) and LC (right panels) were infected with MuV as described in (D). Intracellular MuV-NP (red) was localized by IF staining at 48 h. Cellular plasma and nuclei were visualized using IF for alpha-tubulin (green) and DAPI (blue) counterstaining, respectively. (F) MuV loads in culture medium. SC and LC were infected with MuV as described in (D). Virus loads in culture media were measured by TCID50 assay at 48 h after infection. (G) MuV replication in vivo, MuV (1 107 PFU) in 10 µL of PBS was injected into each testis of 8-week-old WT and Axl -/- Mer -/- mice (n=3 mice each group). Total RNA was extracted from the testis and relative RNA level of MuV-NP was {KO}

产品信息

14-5751-82

应用

建议稀释比

已发表文章

免疫印迹 (WB)

Assay-Dependent

查看1篇已发表文章 1篇已发表文章

免疫组化 (IHC)

查看5篇已发表文章 5篇已发表文章

免疫组化（冰冻） (IHC (F))

5 µg/mL

查看1篇已发表文章 1篇已发表文章

免疫细胞化学 (ICC/IF)

5 µg/mL

查看2篇已发表文章 2篇已发表文章

流式细胞分析 (Flow)

Assay-Dependent

查看6篇已发表文章 6篇已发表文章

免疫沉淀 (IP)

Assay-Dependent

查看1篇已发表文章 1篇已发表文章

产品规格

种属反应

Mouse

已发表种属

Mouse

宿主/亚型

Rat / IgG2a, kappa

分类

Monoclonal

类型

Antibody

克隆号

DS5MMER

抗原

FC-tagged full protein expressed in insect cells

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2

内含物

0.09% sodium azide

保存条件

4° C

运输条件

Ambient (domestic); Wet ice (international)

AB_2688282

产品详细信息

Description: This DS5MMER monoclonal antibody recognizes mouse MerTK, a 170-210 kDa member of the TAM family of tyrosine kinase receptors that also includes Axl and Tyro3. MerTK is expressed on tissue macrophages and is involved in the removal of apoptotic cells. This process relies on two soluble ligands of MerTK, Protein S and Gas6 that bind to phosphatidylserine found on the outer leaflet of the plasma membrane of cells undergoing apoptosis. Upon binding these ligands, MerTK undergoes autophosphorylation at multiple tyrosine residues that activate the PI3K and Akt pathways. This results in the phagocytosis of apoptotic cells and also results in the direct inhibition of TLR-induced production of pro-inflammatory cytokines. In addition, MerTK may function as a putative entry receptor for filoviruses. Deficiency of MerTK causes general autoimmunity, inflammation and accumulation of apoptotic bodies. MerTK is constitutively released from the cell surface by metalloproteinases and thus is present in the serum and culture medium. This process can be enhanced by stimulation with LPS. MerTK can be used to help discriminate macrophages from dendritic cells. MerTK is often expressed on malignant cells and may be implicated in immune evasion.

The DS5MMER antibody will work on paraformaldehyde-fixed cells. In addition, this antibody was demonstrated to inhibit Gas6-induced MerTK phosphorylation with the IC50 < 5 nM. The DS5MMER antibody also works in western blotting and immunoprecipitation.

Applications Reported: This DS5MMER antibody has been reported for use in flow cytometric analysis, immunohistochemical staining, immunohistochemical staining of frozen tissue sections, western blotting and immunoprecipitation. Fluorochrome-conjugated DS5MMER is recommended for use in flow cytometry.

Applications Tested: This DS5MMER antibody has been tested by immunohistochemistry on frozen mouse tissue and can be used at less than or equal to 5 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

MERTK (cMER) is a tyrosine kinase proto-oncogene and is involved in the retinal pigment epithelium (RPE) phagocytosis pathway, which is implicated in human retinal disease. Regulates many physiological processes including cell survival, migration, differentiation, and phagocytosis of apoptotic cells (efferocytosis). Ligand binding at the cell surface induces autophosphorylation of MERTK on its intracellular domain that provides docking sites for downstream signaling molecules. Following activation by ligand, interacts with GRB2 or PLCG2 and induces phosphorylation of MAPK1, MAPK2, FAK/PTK2 or RAC1. MERTK signaling plays a role in various processes such as macrophage clearance of apoptotic cells, platelet aggregation, cytoskeleton reorganization and engulfment. Functions in the retinal pigment epithelium (RPE) as a regulator of rod outer segments fragments phagocytosis. Plays also an important role in inhibition of Toll-like receptors (TLRs)-mediated innate immune response by activating STAT1, which selectively induces production of suppressors of cytokine signaling SOCS1 and SOCS3.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

AXL Expression on Homeostatic Resident Liver Macrophages Is Reduced in Cirrhosis Following GAS6 Production by Hepatic Stellate Cells.

Cellular and molecular gastroenterology and hepatology

Pop OT,Geng A,Flint E,Singanayagam A,Ercan C,Possamai L,Patel VC,Kuenzler P,Meier MA,Soysal S,Hruz P,Kollmar O,Tatham KC,Ward JK,Müllhaupt B,Weber A,Wendon J,Niess JH,Heim M,Semela D,Weston C,Antoniades CG,Terracciano LM,Triantafyllou E,Brenig RG,Bernsmeier C

Published figure using MERTK monoclonal antibody (Product # 14-5751-82) in Flow Cytometry

Mon Jun 26 00:00:00 UTC 2023

Microglia Maintain Homeostatic Conditions in the Developing Rostral Migratory Stream.

eNeuro

Meller SJ,Hernandez L,Martin-Lopez E,Kloos ZA,Liberia T,Greer CA

14-5751-82 was used in Immunohistochemistry-immunofluorescence to illustrate the importance of microglia in maintaining a healthy neuroblast population and an environment permissive to neuroblast migration in the early postnatal RMS.

Wed Feb 01 00:00:00 UTC 2023

Single-cell transcriptomics reveals distinct cell response between acute and chronic pulmonary infection of Pseudomonas aeruginosa.

MedComm

Hu X,Wu M,Ma T,Zhang Y,Zou C,Wang R,Zhang Y,Ren Y,Li Q,Liu H,Li H,Wang T,Sun X,Yang Y,Tang M,Li X,Li J,Gao X,Li T,Zhou X

14-5751-82 was used in Immunohistochemistry-immunofluorescence to provide a comprehensive and unbiased immune cellular landscape for respiratory pathogenesis in mice, enabling further understanding of immunologic mechanisms in infection and inflammatory diseases.

Thu Dec 01 00:00:00 UTC 2022

Single-cell transcriptomics reveals distinct cell response between acute and chronic pulmonary infection of Pseudomonas aeruginosa.

MedComm

Hu X,Wu M,Ma T,Zhang Y,Zou C,Wang R,Zhang Y,Ren Y,Li Q,Liu H,Li H,Wang T,Sun X,Yang Y,Tang M,Li X,Li J,Gao X,Li T,Zhou X

Thu Dec 01 00:00:00 UTC 2022

Ligand-dependent kinase activity of MERTK drives efferocytosis in human iPSC-derived macrophages.

Cell death & disease

Wanke F,Gutbier S,Rümmelin A,Steinberg M,Hughes LD,Koenen M,Komuczki J,Regan-Komito D,Wagage S,Hesselmann J,Thoma R,Brugger D,Christopeit T,Wang H,Point F,Hallet R,Ghosh S,Rothlin CV,Patsch C,Geering B

Published figure using MERTK monoclonal antibody (Product # 14-5751-82) in Flow Cytometry

Tue May 25 00:00:00 UTC 2021

TAM kinase signaling is indispensable for proper skeletal muscle regeneration in mice.

Cell death & disease

Al-Zaeed N,Budai Z,Szondy Z,Sarang Z

Published figure using MERTK monoclonal antibody (Product # 14-5751-82) in Western Blot

Sat Jun 12 00:00:00 UTC 2021

Chronic Porphyromonas gingivalis lipopolysaccharide induces adverse myocardial infarction wound healing through activation of CD8⁺ T cells.

American journal of physiology. Heart and circulatory physiology

Zaidi Y,Corker A,Vasileva VY,Oviedo K,Graham C,Wilson K,Martino J,Troncoso M,Broughton P,Ilatovskaya DV,Lindsey ML,DeLeon-Pennell KY

14-5751-82 was used in Immunohistochemistry-immunofluorescence to indicate that in response to circulating periodontal endotoxins, memory CD8+ T cells are activated, resulting in an acceleration of macrophage-mediated inflammation after MI.

Mon Nov 01 00:00:00 UTC 2021

Roles of Sialic Acid, AXL, and MER Receptor Tyrosine Kinases in Mumps Virus Infection of Mouse Sertoli and Leydig Cells.

Frontiers in microbiology

Wang F,Chen R,Jiang Q,Wu H,Gong M,Liu W,Yu X,Zhang W,Han R,Liu A,Chen Y,Han D

14-5751 was used in Immunocytochemistry-immunoflourescence to provide novel insights into the mechanisms behind MuV infection and replication in the testis.

Mon Sep 28 00:00:00 UTC 2020

Chimeric apoptotic bodies functionalized with natural membrane and modular delivery system for inflammation modulation.

Science advances

Dou G,Tian R,Liu X,Yuan P,Ye Q,Liu J,Liu S,Zhou J,Deng Z,Chen X,Liu S,Jin Y

14-5751-82 was used in Immunohistochemistry, Immunocytochemistry to provide strategies to arbitrarily engineer modular EVs that integrate the advantages of natural EVs and synthetic materials for various applications.

Wed Jul 01 00:00:00 UTC 2020

Chimeric apoptotic bodies functionalized with natural membrane and modular delivery system for inflammation modulation.

Science advances

Dou G,Tian R,Liu X,Yuan P,Ye Q,Liu J,Liu S,Zhou J,Deng Z,Chen X,Liu S,Jin Y

Wed Jul 01 00:00:00 UTC 2020

Pro-inflammatory Aorta-Associated Macrophages Are Involved in Embryonic Development of Hematopoietic Stem Cells.

Immunity

Mariani SA,Li Z,Rice S,Krieg C,Fragkogianni S,Robinson M,Vink CS,Pollard JW,Dzierzak E

14-5751-82 was used in Immunohistochemistry (Frozen) to show here that macrophage appearance in the AGM was dependent on the chemokine receptor Cx3cr1.

Tue Jun 18 00:00:00 UTC 2019

TAM Family Receptor Kinase Inhibition Reverses MDSC-Mediated Suppression and Augments Anti-PD-1 Therapy in Melanoma.

Cancer immunology research

Holtzhausen A,Harris W,Ubil E,Hunter DM,Zhao J,Zhang Y,Zhang D,Liu Q,Wang X,Graham DK,Frye SV,Earp HS

14-5751 was used in Immunoprecipitation to investigate if myeloid-derived suppressor cells (MDSC) could upregulate TYRO3, AXL, and MERTK and their ligands [monocytic MDSCs (M-MDSC), polymorphonuclear MDSCs (PMN-MDSC)] in tumor-bearing mice.

Tue Oct 01 00:00:00 UTC 2019

Protective Role of the MER Tyrosine Kinase via Efferocytosis in Rheumatoid Arthritis Models.

Frontiers in immunology

Waterborg CEJ,Beermann S,Broeren MGA,Bennink MB,Koenders MI,van Lent PLEM,van den Berg WB,van der Kraan PM,van de Loo FAJ

Published figure using MERTK monoclonal antibody (Product # 14-5751-82) in Flow Cytometry

Thu Jun 13 00:00:00 UTC 2019

iRhom2-mediated proinflammatory signalling regulates heart repair following myocardial infarction.

JCI insight

Barnette DN,Cahill TJ,Gunadasa-Rohling M,Carr CA,Freeman M,Riley PR

Published figure using MERTK monoclonal antibody (Product # 14-5751-82) in Flow Cytometry

Thu Feb 08 00:00:00 UTC 2018

Phosphatidylserine Exposure Controls Viral Innate Immune Responses by Microglia.

Neuron

Tufail Y,Cook D,Fourgeaud L,Powers CJ,Merten K,Clark CL,Hoffman E,Ngo A,Sekiguchi KJ,O'Shea CC,Lemke G,Nimmerjahn A

14-5751 was used in Immunohistochemistry to investigate the mechanisms through which microglia recognise and respond to viral uptake.

Wed Feb 08 00:00:00 UTC 2017

MEK1/2 Inhibition Promotes Macrophage Reparative Properties.

Journal of immunology (Baltimore, Md. : 1950)

Long ME,Eddy WE,Gong KQ,Lovelace-Macon LL,McMahan RS,Charron J,Liles WC,Manicone AM

14575182 was used in flow cytometry to show that the MEK1/2 pathway is a key regulator of macrophage reparative properties

Sun Jan 15 00:00:00 UTC 2017

An Epithelial Integrin Regulates the Amplitude of Protective Lung Interferon Responses against Multiple Respiratory Pathogens.

PLoS pathogens

Meliopoulos VA,Van de Velde LA,Van de Velde NC,Karlsson EA,Neale G,Vogel P,Guy C,Sharma S,Duan S,Surman SL,Jones BG,Johnson MD,Bosio C,Jolly L,Jenkins RG,Hurwitz JL,Rosch JW,Sheppard D,Thomas PG,Murray PJ,Schultz-Cherry S

14-5751 was used in Flow cytometry/Cell sorting to show that upregulation of the epithelial integrin αVβ6 during influenza infection is involved in disease pathogenesis.

Mon Aug 01 00:00:00 UTC 2016

生物信息学

蛋白别名: MGC133349; Proto-oncogene c-Mer; proto-oncogene tyrosine-protein kinase Mer; Receptor tyrosine kinase MerTK; sMER; sMERTK; soluble MER; soluble MERTK; Tyro 12; Tyrosine-protein kinase Mer

查看更多收起

基因别名: Eyk; Mer; Mertk; nmf12; Nyk

查看更多收起

UniProt ID: (Mouse) Q60805

查看更多收起

Entrez Gene ID: (Mouse) 17289

查看更多收起

功能

nucleotide binding protein kinase activity protein tyrosine kinase activity transmembrane receptor protein tyrosine kinase activity ATP binding kinase activity transferase activity transferase activity, transferring phosphorus-containing groups transmembrane signal receptor

参与通路