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MLCK Antibody (PA5-100908) in ICC/IF
Immunocytochemistry analysis of MLCK in A549 cells. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were incubated with MLCK polyclonal antibody (Product # PA5-100908) and mouse anti-beta tubulin antibody for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) (Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) (Green) were used as the secondary antibodies. The nuclear counter stain is DAPI (blue).
产品信息
PA5-100908
产品规格
种属反应
Human,
Mouse,
Rat
已发表种属
Rat
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
A synthesized peptide derived from human MYLK3(Accession Q32MK0), corresponding to amino acid residues L204-P254.
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Affinity chromatography
保存液
PBS, pH 7.4, with 50% glycerol
内含物
0.02% sodium azide
保存条件
-20°C
运输条件
Wet ice
RRID
AB_2850401
产品详细信息
Antibody detects endogenous levels of total MYLK3.
靶标信息
MLCK (MLCK2), now also known as MYLK3, is of the myosin regulatory light chain kinases which facilitates muscle contraction. MLCK, a member of the Ser/Thr protein kinase family, is responsible for smooth muscle contraction via phosphorylation of a specific serine in the N-terminus of myosin light chains (MLC), an event that facilitates myosin interaction with actin filaments. It is a central determinant in the development of vascular permeability and tissue edema formation. In the nervous system it has been shown to control the growth initiation of astrocytic processes in culture and to participate in transmitter release at synapses formed between cultured sympathetic ganglion cells. MLCK acts as a critical participant in signaling sequences that result in fibroblast apoptosis. Smooth muscle and non-muscle isozymes are expressed in a wide variety of adult and fetal tissues and in cultured endothelium with qualitative expression appearing to be neither tissue- nor development-specific. Non-muscle isoform 2 is the dominant splice variant expressed in various tissues. The Telokin isoform, which binds calmodulin, has been found in a wide variety of adult and fetal tissues. MLCK is probably down-regulated by phosphorylation. The protein contains 1 fibronectin type III domain and 9 immunoglobulin-like C2-type domains.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: Cardiac MLCK; Cardiac MyBP-C-associated Ca/CaM kinase; Cardiac-MLCK; cardiac-MyBP-C associated Ca/CaM kinase; Cardiac-MyBP-C-associated Ca/CaM kinase; cardiac-specific myosin light chain kinase; MLC kinase; Myosin light chain kinase 3; putative myosin light chain kinase 3
基因别名: caMLCK; D830007F02Rik; MLCK; MLCK2; MYLK3; RGD1305801
UniProt ID: (Human) Q32MK0, (Mouse) Q3UIZ8, (Rat) E9PT87
Entrez Gene ID: (Human) 91807, (Mouse) 213435, (Rat) 291926
calmodulin-dependent protein kinase activity
myosin light chain kinase activity
ATP binding
nucleotide binding
protein kinase activity
protein serine/threonine kinase activity
kinase activity
transferase activity
transferase activity, transferring phosphorus-containing groups
non-receptor serine/threonine protein kinase
