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MMP16 Antibody (701306) in ICC/IF
Immunofluorescent analysis of MMP16 was performed on 80% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0. 25% Triton X-100 for 10 minutes, and blocked with 5% BSA for 1 hour at room temperature. The cells were labeled with MMP16 Recombinant Rabbit Monoclonal Antibody (Product # 701306) at a dilution of 1:1000 in 1% BSA and incubated for 3 hours at room temperature and then labeled with Alexa Fluor® 488 Goat anti-Rabbit IgG secondary antibody (Product # A-11008) at a dilutio... View More
产品信息
701306
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Rat
已发表种属
Human
宿主/亚型
Rabbit
/ IgG
Expression System
Expi293
分类
Recombinant Monoclonal
类型
Antibody
克隆号
13H7L7
抗原
Recombinant protein corresponding to amino acids 121-545 of human MMP-16
偶联物
形式
Liquid
浓度
0.5 mg/mL
纯化类型
Protein A
保存液
PBS
内含物
0.09% sodium azide
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Wet ice
RRID
AB_2532467
产品详细信息
Intact IgG appears on a non-reducing gel as ~150 kDa band and upon reduction generating a ~25 kDa light chain band and a ~50 kDa heavy chain.
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
靶标信息
Matrix metallopeptidase 16 during embryonic development, reproduction, and tissue remodeling. MMP-16 is also involved in the ECM degradation processes associated with arthritis and metastasis. In humans, MMP-16 is a part of a cluster of MMP genes at the 11q22.3 locus.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: DKFZp761D112; matrix metallo protease; matrix metallopeptidase 16 (membrane-inserted); matrix metalloproteinase 16; Matrix metalloproteinase-16; Membrane-type matrix metalloproteinase 3; Membrane-type-3 matrix metalloproteinase; MMP; MMP-1; MMP-16; MMP-X2; MMPs; MT-MMP 3; MT3-MMP; MT3MMP; MTMMP3; Putative transmembrane protein C8orf57
基因别名: C8orf57; MMP-X2; MMP16; MMPX2; MT-MMP2; MT-MMP3; MT3-MMP
UniProt ID: (Human) P51512, (Rat) O35548
Entrez Gene ID: (Human) 4325, (Rat) 65205
