Invitrogen

MMP9 Recombinant Rabbit Monoclonal Antibody (JA80-73)

MMP9 Antibody in Immunocytochemistry (ICC/IF)

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MMP9 Antibody (MA5-32705) in ICC/IF

Immunofluorescence analysis of Matrix metalloproteinase-9 was performed using 70% confluent log phase THP-1 cells differentiated to Macrophages using PMA treatment (100nM, 48 hours). The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with MMP9 Recombinant Rabbit Monoclonal Antibody (JA80-73) (Product # MA5-32705) at 1:100 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then la... View More

MMP9 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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MMP9 Antibody — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. THP-1 cells were transfected with Matrix metalloproteinase-9 siRNA and decrease in signal intensity was observed in Western Blot application using Anti-MMP9 Recombinant Rabbit Monoclonal Antibody (JA80-73) (Product # MA5-32705). {KD}

产品信息

MA5-32705

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:5,000

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免疫组化 (IHC)

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免疫组化（石蜡） (IHC (P))

1:1,000

免疫细胞化学 (ICC/IF)

1:50-1:200

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流式细胞分析 (Flow)

1:50-1:100

产品规格

种属反应

Human, Rat

已发表种属

Human, Rat

宿主/亚型

Rabbit / IgG

Expression System

HEK293 cells

分类

Recombinant Monoclonal

类型

Antibody

克隆号

JA80-73

抗原

Synthetic peptide within Human MMP9 aa 71-120

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Protein A

保存液

TBS, pH 7.4, with 40% Glycerol, 0.05% BSA

内含物

0.05% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

AB_2809982

产品详细信息

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

靶标信息

MMP9 (matrix metallopeptidase 9, GELB, CLG4B) is a matrix metalloproteinase, a family of zinc and calcium-dependent endopeptidases that degrade extracellular matrix proteins. MMP9 is secreted as a 92kDa zymogen and cleavage of pro-MMP9 results in the active enzyme with a molecular weight of 82kDa. MMP9 has a gelatin-binding domain consisting of three fibronectin type II units, a catalytic domain containing the zinc-binding site, a proline-rich type V collagen-homologous domain and a hemopexin-like domain. MMP9 is produced by monocytes, macrophages, neutrophils, keratinocytes, fibroblasts, osteoclasts and endothelial cells, and is involved in inflammatory responses, tissue remodeling, wound healing, tumor growth and metastasis. MMP9 is supplied by bone marrow-derived cells and contributes to skin carcinogenesis. Further, MMP9 degrades type IV and V collagens. Studies in rhesus monkeys suggest that the enzyme is involved in IL-8-induced mobilization of hematopoietic progenitor cells from bone marrow, and murine studies suggest a role in tumor-associated tissue remodeling. Studies have shown that elevated MMP9 is associated with progression of idiopathic atrial fibrillation and aortic aneurysm.

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