Invitrogen

MRPL15 Polyclonal Antibody

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MRPL15 Antibody (PA5-109975) in WB

Western blot analysis of MRPL15 in extracts of various cell lines. Samples were incubated with MRPL15 Polyclonal antibody (Product # PA5-109975) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

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产品信息

PA5-109975

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:2,000

酶联免疫吸附实验 (ELISA)

1 µg/mL

产品规格

种属反应

Human, Mouse

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Recombinant fusion protein containing a sequence corresponding to amino acids 1-296 of human MRPL15 (NP_054894.1).

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1.95 mg/mL

纯化类型

Affinity Chromatography

保存液

PBS, pH 7.3, with 50% glycerol

内含物

0.01% thimerosal

保存条件

-20° C, Avoid Freeze/Thaw Cycles

运输条件

Wet ice

RRID

AB_2855386

产品详细信息

Immunogen sequence: MAGPLQGGGA RALDLLRGLP RVSLANLKPN PGSKKPERRP RGRRRGRKCG RGHKGERQRG TRPRLGFEGG QTPFYIRIPK YGFNEGHSFR RQYKPLSLNR LQYLIDLGRV DPSQPIDLTQ LVNGRGVTIQ PLKRDYGVQL VEEGADTFTA KVNIEVQLAS ELAIAAIEKN GGVVTTAFYD PRSLDIVCKP VPFFLRGQPI PKRMLPPEEL VPYYTDAKNR GYLADPAKFP EARLELARKY GYILPDITKD ELFKMLCTRK DPRQIFFGLA PGWVVNMADK KILKPTDENL LKYYTS

靶标信息

Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein that belongs to the EcoL15 ribosomal protein family. A pseudogene corresponding to this gene is found on chromosome 15q.

⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

仅用于科研。不用于诊断过程。未经明确授权不得转售。