Invitrogen

MSI2 Polyclonal Antibody

MSI2 Antibody in Immunocytochemistry (ICC/IF)

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MSI2 Antibody (PA5-31024) in ICC/IF

Immunofluorescence analysis of Lysozyme was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with MSI2 Polyclonal Antibody (Product # PA5-31024) at 1:100 dilution in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Recombinant Secondary Antibody, Alexa Fluor® 488 conjugate (P... View More

MSI2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

MSI2 Antibody in Immunohistochemistry (Frozen) (IHC (F))

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MSI2 Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across cells and tissues tested owing to their inherent genetic constitution. Relative expression of MSI2 was observed in HeLa, SH-SY5Y, K-562, HEL 92.1.7, Neuro-2a, PC-12 and Mouse Brain in comparison to U-937 and Mouse Kidney by using Anti-MSI2 Polyclonal Antibody (Product # PA5-31024) (10.1371/journal.pone.0136484). {RE}

产品信息

PA5-31024

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:3,000

免疫组化（石蜡） (IHC (P))

1:100-1:1,000

免疫组化（冰冻） (IHC (F))

1:100-1:1,000

免疫细胞化学 (ICC/IF)

1:100

免疫沉淀 (IP)

Assay-dependent

产品规格

种属反应

Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Recombinant fragment corresponding to a region within amino acids 1 and 217 of Human Musashi 2

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS, pH 7, with 1% BSA, 20% glycerol

内含物

0.01% thimerosal

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

AB_2548498

产品详细信息

Recommended positive controls: IMR32, MCF-7, mouse brain.

Predicted reactivity: Mouse (100%), Xenopus laevis (97%), Bovine (84%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

靶标信息

Msi2 (musashi homolog 2), also known as MSI2H, is a 328 amino acid protein that localizes to the cytoplasm and contains two RRM (RNA recognition motif) domains. Expressed ubiquitously at low levels, Msi2 functions as an RNA binding protein that, by regulating the expression of target mRNAs, is thought to play a role in the proliferation and maintenance of stem cells within the central nervous system. Msi2 is subject to posttranslational phosphorylation and is upregulated in response to brain injury, suggesting a role in healing and brain tissue regeneration. Chromosomal aberrations involving the Msi2 gene are associated with the progression of chronic myeloid leukemia. Multiple isoforms of Msi2 exist due to alternative splicing events.Tissue specificity: Ubiquitous; detected at low levels.

⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

仅用于科研。不用于诊断过程。未经明确授权不得转售。