Invitrogen

MUC1 Monoclonal Antibody (GP1.4)

MUC1 Antibody in Immunocytochemistry (ICC/IF)

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MUC1 Antibody (MA1-06503) in ICC/IF

Immunofluorescence analysis of MUC1 was performed using 70% confluent log phase T-47D cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with MUC1 Monoclonal Antibody (GP1.4) (Product # MA1-06503) at 1:100 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product ... View More

MUC1 Antibody in Immunohistochemistry (IHC)

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MUC1 Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across T47D, MCF-7, DU-145, HCT-116 and IMR-32 owing to their inherent genetic constitution. Relative expression of MUC1 was observed in increased expression like a streak pattern in T47D, MCF-7 and DU-145 in comparison to HCT116 and IMR-32 using Anti-MUC1 Monoclonal Antibody (GP1.4) (Product # MA1-06503) in Western Blot. {RE}

产品信息

MA1-06503

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:100-1:1,000

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免疫组化 (IHC)

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免疫组化（石蜡） (IHC (P))

1:25-1:200

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免疫组化（冰冻） (IHC (F))

1:25-1:200

免疫细胞化学 (ICC/IF)

Assay-dependent

查看4篇已发表文章 4篇已发表文章

流式细胞分析 (Flow)

1:25-1:200

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产品规格

种属反应

Human

已发表种属

Human, Mouse

宿主/亚型

Mouse / IgG1

分类

Monoclonal

类型

Antibody

克隆号

GP1.4

抗原

Human milk fat globule.

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

purified

保存液

PBS

内含物

0.09% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Ambient (domestic); Wet ice (international)

AB_558126

产品详细信息

MA1-06503 detects episialin in human samples.

MA1-06503 has sucessfully been used in Western blotting, flow cytometry, immunocytochemistry, and immunohistochemistry.

The MA1-06503 immunogen is a human milk fat globule.

Store at 4ºC or in small aliquots at -20ºC if preferred. Avoid freeze-thaw cycles.

靶标信息

MUC1 (Mucin 1, Episialin, MAM-6, CA 15-3, PEM and EMA) is a large cell surface mucin glycoprotein expressed by most glandular and ductal epithelial cells and some hematopoietic cell lineages. MUC1 is a transmembrane glycoprotein with a large mucin-like extracellular domain that matures through several intermediate forms generated by proteolysis, and sequential addition and processing of numerous O-linked glycans that are heavily sialylated. MUC1 is highly polymorphic and each allele encodes a product that contains a different number of repeats (between 30 and 90) leading to large differences in molecular weight of the protein. MUC1 is expressed on most secretory epithelium, including mammary gland and some hematopoietic cells, and is expressed abundantly in >90% breast carcinomas and metastases. Transgenic MUC1 has been shown to associate with all four cebB receptors and localize with erbB1 (EGFR) in lactating glands. The MUC1 gene contains seven exons and produces several different alternatively spliced variants. Overexpression, aberrant intracellular localization, and changes in glycosylation of the MUC1 protein have been associated with carcinomas. Multiple alternatively spliced transcript variants that encode different isoforms of the MUC1 gene have been reported, but the full-length nature of only some has been determined. Transgenic MUC-1 has been shown to associate with all four cebB receptors and localize with erbB1 (EGFR) in lactating glands.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

The sialidase NEU1 directly interacts with the juxtamembranous segment of the cytoplasmic domain of mucin-1 to inhibit downstream PI3K-Akt signaling.

The Journal of biological chemistry

Hyun SW,Imamura A,Ishida H,Piepenbrink KH,Goldblum SE,Lillehoj EP

MA1-06503 was used in Flow Cytometry, Immunocytochemistry, Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin), Western Blot to indicate that NEU1 associates with the juxtamembranous region of the MUC1-CD to inhibit PI3K-Akt signaling independent of NEU1 catalytic activity.

Mon Nov 01 00:00:00 UTC 2021

Neuraminidase 1-mediated desialylation of the mucin 1 ectodomain releases a decoy receptor that protects against Pseudomonas aeruginosa lung infection.

The Journal of biological chemistry

Lillehoj EP,Guang W,Hyun SW,Liu A,Hegerle N,Simon R,Cross AS,Ishida H,Luzina IG,Atamas SP,Goldblum SE

MA1-06503 was used in Flow Cytometry, Immunocytochemistry, Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin), Western Blot to investigate MUC1-ED desialylation in lung infection.

Fri Jan 11 00:00:00 UTC 2019

Membrane-Tethered MUC1 Mucin Counter-Regulates the Phagocytic Activity of Macrophages.

American journal of respiratory cell and molecular biology

Kato K,Uchino R,Lillehoj EP,Knox K,Lin Y,Kim KC

MA106503 was used in western blot to investigate the role of MUC1 on macrophage differentiation or phagocytic activity

Fri Apr 01 00:00:00 UTC 2016

Mucin1 shifts Smad3 signaling from the tumor-suppressive pSmad3C/p21(WAF1) pathway to the oncogenic pSmad3L/c-Myc pathway by activating JNK in human hepatocellular carcinoma cells.

Oncotarget

Li Q,Liu G,Yuan H,Wang J,Guo Y,Chen T,Zhai R,Shao D,Ni W,Tai G

MA1-06503 was used in flow cytometry, immunohistochemistry - paraffin section, and western blot to study the shift of Smad3 signaling from the tumor-suppressive pSmad3C/p21(WAF1) pathway to the oncogenic pSmad3L/c-Myc pathway occurs by Mucin1 activating J

Sat Feb 28 00:00:00 UTC 2015

Mucin1 shifts Smad3 signaling from the tumor-suppressive pSmad3C/p21(WAF1) pathway to the oncogenic pSmad3L/c-Myc pathway by activating JNK in human hepatocellular carcinoma cells.

Oncotarget

Li Q,Liu G,Yuan H,Wang J,Guo Y,Chen T,Zhai R,Shao D,Ni W,Tai G

Sat Feb 28 00:00:00 UTC 2015

Expression of mesothelioma-related markers in meningiomas: an immunohistochemical study.

BioMed research international

Abdelzaher E,Abdallah DM

MA1-06503 was used in immunohistochemistry - paraffin section to compare meningiomas and mesotheliomas immunohistochemically

Thu Feb 12 00:00:00 UTC 2015

Multiple oncocytic cystadenoma with intraluminal crystalloids in parotid gland: case report.

Medicine

Başak K,Kroğlu K

MA1-06503 was used in immunohistochemistry to describe a tumor from a patient with oncocytic cystadenoma

Mon Dec 01 00:00:00 UTC 2014

Expression of human full-length MUC1 inhibits the proliferation and migration of a B16 mouse melanoma cell line.

Oncology reports

Wang F,Li Q,Ni W,Fang F,Sun X,Xie F,Wang J,Wang F,Gao S,Tai G

MA1-06503 was used in immunocytochemistry and western blot to study the mechanisms by which expression of full length human MUC1 by the murine B16 melanoma cell inhibits its proliferation and invasiveness

Mon Jul 01 00:00:00 UTC 2013

Multinodular reticular schwannoma in the head and neck region: a potential diagnostic pitfall.

International journal of surgical pathology

Lau PP,Yau DT,Lau WH,Mak LS,Chan JK

MA1-06503 was used in immunohistochemistry to report on a case of multinodular reticular schwannoma in the head and neck region

Fri Feb 01 00:00:00 UTC 2013

Anti-MUC1 antibody inhibits EGF receptor signaling in cancer cells.

Biochemical and biophysical research communications

Hisatsune A,Nakayama H,Kawasaki M,Horie I,Miyata T,Isohama Y,Kim KC,Katsuki H

MA1-06503 was used in immunocytochemistry to study the mechanism by which an anti-MUC1 antibody inhibits EGF receptor signaling in cancer cells

Fri Feb 18 00:00:00 UTC 2011

Human embryonic stem cell-derived keratinocytes exhibit an epidermal transcription program and undergo epithelial morphogenesis in engineered tissue constructs.

Tissue engineering. Part A

Metallo CM,Azarin SM,Moses LE,Ji L,de Pablo JJ,Palecek SP

MA1-06503 was used in immunohistochemistry to examine the epithelial differentiation of human embryonic stem cells

Fri Jan 01 00:00:00 UTC 2010

Secondary involvement of the breast in T-cell non-Hodgkin lymphoma, an unusual example mimicking inflammatory breast carcinoma.

Archives of gynecology and obstetrics

Kelten C,Kabukcu S,Sen N,Teke Z,Yaren A,Erdem E,Duzcan E

MA1-06503 was used in immunohistochemistry to report a clinical case of T-cell non-Hodgkin lymphoma with unusual inflammatory breast carcinoma similarity

Wed Jul 01 00:00:00 UTC 2009

Myointimoma of the glans penis.

Pathology international

Vardar E,Gunlusoy B,Arslan M,Kececi S

MA1-06503 was used in immunohistochemistry to report on a case of myointimoma of the glans penis

Thu Mar 01 00:00:00 UTC 2007

Pulmonary large cell carcinoma with rhabdoid phenotype.

Annals of diagnostic pathology

Yilmazbayhan D,Ates LE,Dilege S,Gulluoglu M,Tanju S,Kalayci G

MA1-06503 was used in immunohistochemistry The report on a patient with pulmonary large cell carcinoma with rhabdoid phenotype

Mon Aug 01 00:00:00 UTC 2005

Selective secretion and replenishment of discrete mucin glycoforms from intestinal goblet cells.

The American journal of physiology

Stanley CM,Phillips TE

MA1-06503 was used in immunocytochemistry to investigate production and secretion and of mucin glycoforms in HT29-18N2 cells

Thu Jul 01 00:00:00 UTC 1999

生物信息学

蛋白别名: Breast carcinoma-associated antigen DF3; CA 15-3; Cancer antigen 15-3; Carcinoma-associated mucin; CD227; DF3 antigen; EMA; Episialin; H23 antigen; H23AG; J19; KL-6; Krebs von den Lungen-6; Medullary cystic kidney disease, autosomal dominant; MUC-1; MUC1-alpha; MUC1-beta; MUC1-CT; MUC1-NT; mucin 1, transmembrane; Mucin-1; Mucin-1 subunit alpha; Mucin-1 subunit beta; Peanut-reactive urinary mucin; PEM; PEMT; Polymorphic epithelial mucin; PUM; tumor associated epithelial mucin; Tumor-associated epithelial membrane antigen; Tumor-associated mucin

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基因别名: ADMCKD; ADMCKD1; CA 15-3; CD227; EMA; H23AG; KL-6; MAM6; MCD; MCKD; MCKD1; MUC-1; MUC-1/SEC; MUC-1/X; MUC1; MUC1/ZD; PEM; PEMT; PUM

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UniProt ID: (Human) P15941

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Entrez Gene ID: (Human) 4582

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功能

RNA polymerase II core promoter proximal region sequence-specific DNA binding p53 binding transcription cofactor activity protein binding

参与通路

DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest DNA damage response, signal transduction by p53 class mediator resulting in transcription of p21 class mediator negative regulation of transcription by competitive promoter binding O-glycan processing negative regulation of cell adhesion mediated by integrin positive regulation of transcription from RNA polymerase II promoter in response to stress regulation of transcription from RNA polymerase II promoter in response to stress positive regulation of histone H4 acetylation negative regulation of intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator