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Marginal Zone B Cells Antibody (14-9489-82) in IHC (F)
Immunohistochemistry of frozen rat spleen using 5 µg/mL of Anti-Rat Marginal Zone B Cells Purified, followed by F (ab')2 Anti-Mouse IgG eFluor® 660 (white), Anti-Rat CD3 Biotin, followed by Streptavidin eFluor® 570 (red), and Anti-Rat CD45R (B220) FITC (green).Nuclei are stained with DAPI (blue).
产品信息
14-9489-82
应用
建议稀释比
已发表文章
产品规格
种属反应
Rat
宿主/亚型
Mouse
/ IgG1, kappa
分类
Monoclonal
类型
Antibody
克隆号
HIS57
偶联物
形式
Liquid
浓度
0.5 mg/mL
纯化类型
Affinity chromatography
保存液
PBS, pH 7.2
内含物
0.09% sodium azide
保存条件
4° C
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_2572947
产品详细信息
Description: The monoclonal antibody HIS57 recognizes an unknown antigen expressed by rat marginal zone B cells. Marginal zone B cells are an anatomical B cell compartment located in the spleen at the area between red and white pulp. Marginal zone B cells serve as a first line of defense against blood-borne antigens in a T cell-independent manner. Marginal zone B cells can also be phenotyped as B cells that are IgM high, IgD low, CD21 high and CD23 low.
Applications Reported: This HIS57 antibody has been reported for use in flow cytometric analysis, and immunohistochemical staining of frozen tissue sections.
Applications Tested: This HIS57 antibody has been tested by immunohistochemistry of frozen rat tissue and can be used at less than or equal to 5 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Purity: Greater than 90%, as determined by SDS-PAGE.
Aggregation: Less than 10%, as determined by HPLC.
Filtration: 0.2 µm post-manufacturing filtered.
靶标信息
Marginal zone B cells are an anatomical B cell compartment located in the spleen at the area between red and white pulp. Marginal zone B cells serve as a first line of defense against blood-borne antigens in a T cell-independent manner. Marginal zone B cells can also be phenotyped as B cells that are IgM high, IgD low, CD21 high and CD23 low.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: MZ-B; MZB1
