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MiTF Antibody (MA5-16214) in IHC (P)
Immunohistochemical analysis of MiTF in formalin-fixed paraffin-embedded tissue section of human skin. Samples were incubated in MiTF monoclonal antibody (Product # MA5-16214) using a dilution of 1:200 followed by HRP labeled anti-mouse secondary antibody and DAB reagent. Nuclei of cells were counter-stained with hematoxylin. General cytoplasmic staining appears enriched in melanocytes below the epidermis. ... View More
产品信息
MA5-16214
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse,
Rat
已发表种属
Not Applicable
宿主/亚型
Mouse
/ IgG1
分类
Monoclonal
类型
Antibody
克隆号
21D1418
抗原
A synthetic peptide mapping to the 300-350 amino acid region of MITF.
偶联物
形式
Liquid
浓度
1.0 mg/mL
纯化类型
Protein G
保存液
PBS
内含物
0.05% sodium azide
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_2537732
产品详细信息
Suggested positive control: A375 or RAW.
靶标信息
Mi is a basic helix-loop-helix-leucine zipper (b-HLH-ZIP) transcription factor implicated in pigmentation, mast cells and bone development. The mutation of Mi causes Waardenburg Syndrome type II in humans. In mice, a profound loss of pigmented cells in the skin eye and inner ear results, as well as osteopetrosis and defects in natural killer and mast cells. There are two known isoforms of Mi differing by 66 amino acids at the NH2 terminus. Shorter forms are expressed in melanocytes and run as two bands at 52 kDa and 56 kDa, while the longer Mi form runs as a cluster of bands at 60-70 kDa in osteoclasts and in B16 melonoma cells (but not other melanoma cell lines), as well as mast cells and heart.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: bHLHe32; black eyed white; Class E basic helix-loop-helix protein 32; microphtalmia-associated transcription factor; microphthalmia; Microphthalmia-associated transcription factor
基因别名: BCC2; BHLHE32; Bw; CMM8; Gsfbcc2; MI; MITF; Vit; Vitiligo; Wh; WS2; WS2A
UniProt ID: (Human) O75030, (Rat) O88368, (Mouse) Q08874
Entrez Gene ID: (Human) 4286, (Rat) 25094, (Mouse) 17342
RNA polymerase II core promoter proximal region sequence-specific DNA binding
transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding
transcriptional repressor activity, RNA polymerase II transcription regulatory region sequence-specific binding
protein binding
protein dimerization activity
RNA polymerase II core promoter sequence-specific DNA binding
DNA binding
chromatin binding
transcription factor activity, sequence-specific DNA binding
transcription factor activity, RNA polymerase II distal enhancer sequence-specific binding
sequence-specific DNA binding
negative regulation of transcription from RNA polymerase II promoter
regulation of transcription, DNA-templated
transcription from RNA polymerase II promoter
protein complex assembly
multicellular organism development
positive regulation of gene expression
protein sumoylation
melanocyte differentiation
regulation of apoptotic process
regulation of osteoclast differentiation
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
mast cell migration
positive regulation of DNA-templated transcription, initiation
regulation of RNA biosynthetic process
transcription, DNA-templated
regulation of transcription from RNA polymerase II promoter
regulation of gene expression
Wnt signaling pathway
cell differentiation
osteoclast differentiation
regulation of cell proliferation
camera-type eye development
negative regulation of apoptotic process
pigmentation
canonical Wnt signaling pathway involved in negative regulation of apoptotic process
cell fate commitment
bone remodeling
