Invitrogen

Mu-Calpain Monoclonal Antibody (15C10)

Mu-Calpain Antibody in Western Blot (WB)

图: 1 / 5

Mu-Calpain Antibody (MA1-12434) in WB

Western blot analysis was performed on membrane enriched extracts (30 µg lysate) of T-47D (Lane 1), MDA-MB-231 (Lane 2), U-2 OS (Lane 3), U-937 (Lane 4), HEK293T (Lane 5), MCF7 (Lane 6), MOLT-4 (Lane 7) and HeLa (Lane 8). The blot was probed with Anti-Mu-Calpain Monoclonal Antibody (15C10) (Product # MA1-12434, 1 µg/ml) and detected by chemiluminescence using Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A28177, 0.25 µg/ml, 1:4000 dilution). An 80 kDa band corresponding to Mu-Calpain was observed acros... View More

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产品信息

MA1-12434

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1 µg/mL

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免疫组织化学（多聚甲醛固定） (IHC (PFA))

查看1篇已发表文章 1篇已发表文章

流式细胞分析 (Flow)

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酶联免疫吸附实验 (ELISA)

0.5-1.0 µg/mL

免疫沉淀 (IP)

1.0-2.0 µg/mL

产品规格

种属反应

Bovine, Human, Mouse, Rat

已发表种属

Human, Mouse

宿主/亚型

Mouse / IgG1

分类

Monoclonal

类型

Antibody

克隆号

15C10

抗原

Human Calpain protein purified from placenta.

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Protein A/G

保存液

PBS with 50% glycerol

内含物

0.08% sodium azide

保存条件

-20° C, Avoid Freeze/Thaw Cycles

运输条件

Wet ice

AB_2069339

靶标信息

The calpains including Calpain 1 (CAPN1), calcium-activated neutral proteases, are nonlysosomal, intracellular cysteine proteases. The mammalian calpains include ubiquitous, stomach-specific, and muscle-specific proteins. The ubiquitous enzymes consist of heterodimers with distinct large, catalytic subunits associated with a common small, regulatory subunit. This gene encodes the large subunit of the ubiquitous enzyme, calpain 1. Several transcript variants encoding two different isoforms have been found for this gene. Diseases associated with CAPN1 include Spastic Paraplegia 76, Autosomal Recessive and Spasticity.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Muscle calcium stress cleaves junctophilin1, unleashing a gene regulatory program predicted to correct glucose dysregulation.

eLife

Tammineni ER,Figueroa L,Manno C,Varma D,Kraeva N,Ibarra CA,Klip A,Riazi S,Rios E

MA1-12434 was used in Immunohistochemistry and Western Blotting to demonstrate that a JPh44-like construct induces transcriptional changes predictive of increased glucose utilization in myoblasts, including less transcription and translation of GSK3β and decreased transcription of proteins that reduce utilization of glucose.

Wed Feb 01 00:00:00 UTC 2023

Muscle calcium stress cleaves junctophilin1, unleashing a gene regulatory program predicted to correct glucose dysregulation.

eLife

Tammineni ER,Figueroa L,Manno C,Varma D,Kraeva N,Ibarra CA,Klip A,Riazi S,Rios E

Wed Feb 01 00:00:00 UTC 2023

Muscle calcium stress cleaves junctophilin1, unleashing a gene regulatory program predicted to correct glucose dysregulation.

eLife

Tammineni ER,Figueroa L,Manno C,Varma D,Kraeva N,Ibarra CA,Klip A,Riazi S,Rios E

Wed Feb 01 00:00:00 UTC 2023

Muscle calcium stress cleaves junctophilin1, unleashing a gene regulatory program predicted to correct glucose dysregulation.

eLife

Tammineni ER,Figueroa L,Manno C,Varma D,Kraeva N,Ibarra CA,Klip A,Riazi S,Rios E

Wed Feb 01 00:00:00 UTC 2023

Moringa Oleifera Alleviates Homocysteine-Induced Alzheimer's Disease-Like Pathology and Cognitive Impairments.

Journal of Alzheimer's disease : JAD

Mahaman YAR,Huang F,Wu M,Wang Y,Wei Z,Bao J,Salissou MTM,Ke D,Wang Q,Liu R,Wang JZ,Zhang B,Chen D,Wang X

Published figure using Mu-Calpain monoclonal antibody (Product # MA1-12434) in Western Blot

Mon Jun 10 00:00:00 UTC 2019

Calcium dysregulation, functional calpainopathy, and endoplasmic reticulum stress in sporadic inclusion body myositis.

Acta neuropathologica communications

Amici DR,Pinal-Fernandez I,Mázala DA,Lloyd TE,Corse AM,Christopher-Stine L,Mammen AL,Chin ER

MA112434 was used in western blot to determine the role of calcium regulation in sporadic inclusion body myositis

Wed Mar 22 00:00:00 UTC 2017

Roles of calpain-calpastatin system (CCS) in human T cell activation.

Oncotarget

Mikosik A,Jasiulewicz A,Daca A,Henc I,Frąckowiak JE,Ruckemann-Dziurdzińska K,Foerster J,Le Page A,Bryl E,Fulop T,Witkowski JM

MA1-12434 was used in Flow Cytometry to propose that the constitutive, self-regulatory calpain-calpastatin system activity in resting human T cells is a necessary, controlling element of their readiness for complex and effective response to antigenic challenge.

Tue Nov 22 00:00:00 UTC 2016

生物信息学

蛋白别名: Calcium-activated neutral proteinase 1; calpain 1, (mu/I) large subunit; Calpain mu-type; calpain, large polypeptide L1; Calpain-1 catalytic subunit; Calpain-1 large subunit; Calpain-I; Calpain-II; CANP 1; Cell proliferation-inducing gene 30 protein; cell proliferation-inducing protein 30; micromolar calcium activated neutral protease 1; micromolar calcium-activated neutral protease 1; Micromolar-calpain; mu-calpain; muCANP

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基因别名: BOS_24946; CANP; CANP1; CANPL1; Capa-1; Capa1; CAPN1; Cls1; mu-calpin; muCANP; muCL; PIG30; SPG76