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Myogenin Antibody (PA5-78067) in ICC/IF
Immunofluorescence analysis of Myogenin was performed using 70% confluent log phase RD cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with Myogenin Rabbit Polyclonal Antibody (Product # PA5-78067) at 5 µg/mL in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Goat anti-Rabbit IgG (Heavy Chain), Superclonal™ Recombinant Secondary Antibody, Alexa Fluor 488 (Product #... View More
产品信息
PA5-78067
应用
建议稀释比
已发表文章
产品规格
种属反应
Human
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Recombinant protein encompassing a sequence within the center region of human Myogenin. The exact sequence is proprietary.
偶联物
形式
Liquid
浓度
0.51 mg/mL
纯化类型
Antigen affinity chromatography
保存液
PBS, pH 7, with 20% glycerol
内含物
0.025% ProClin 300
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Wet ice
RRID
AB_2736181
产品详细信息
Positive Control: RD
Predicted Reactivity: Mouse (95%), Rat (95%), Pig (95%), Rabbit (96%), Sheep (92%), Bovine (92%)
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
靶标信息
Myogenin is a muscle-specific transcription factor that can induce myogenesis in a variety of cell types in tissue culture. It is a member of a large family of proteins related by sequence homology, the helix-loop-helix proteins. It is essential for the development of functional skeletal muscle.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: bHLHc3; Class C basic helix-loop-helix protein 3; Myf-4; Myogenic factor 4; Myogenic factor-4; Myogenin
基因别名: BHLHC3; myf-4; MYF4; MYOG
UniProt ID: (Human) P15173
Entrez Gene ID: (Human) 4656
RNA polymerase II regulatory region sequence-specific DNA binding
RNA polymerase II core promoter proximal region sequence-specific DNA binding
core promoter binding
transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding
transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding
transcription factor activity, sequence-specific DNA binding
transcription factor activity, RNA polymerase II distal enhancer sequence-specific binding
protein binding
chromatin DNA binding
sequence-specific DNA binding
protein heterodimerization activity
E-box binding
basic helix-loop-helix transcription factor
ossification
cell cycle
skeletal muscle tissue development
negative regulation of cell proliferation
response to gravity
positive regulation of myotube differentiation
skeletal muscle atrophy
positive regulation of muscle atrophy
regulation of skeletal muscle satellite cell proliferation
response to muscle activity involved in regulation of muscle adaptation
response to electrical stimulus involved in regulation of muscle adaptation
striated muscle atrophy
response to denervation involved in regulation of muscle adaptation
skeletal muscle cell differentiation
muscle cell fate commitment
mRNA transcription from RNA polymerase II promoter
skeletal muscle tissue regeneration
myoblast differentiation
positive regulation of myoblast differentiation
negative regulation of glycolytic process
positive regulation of transcription from RNA polymerase II promoter
skeletal muscle fiber development
positive regulation of skeletal muscle fiber development
positive regulation of muscle cell differentiation
positive regulation of cell cycle arrest
cellular response to magnetism
cellular response to lithium ion
cellular response to retinoic acid
cellular response to growth factor stimulus
cellular response to estradiol stimulus
regulation of myoblast fusion
positive regulation of myoblast fusion
positive regulation of oxidative phosphorylation
