Invitrogen

N-cadherin Polyclonal Antibody

N-cadherin Antibody in Immunocytochemistry (ICC/IF)

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N-cadherin Antibody (PA5-19486) in ICC/IF

Immunofluorescence analysis of N-cadherin was performed using 90% confluent log phase SH-SY5Y cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with N-cadherin Monoclonal Antibody (Product # PA5-19486) at 5 µg/mL in 0.1% BSA, incubated at 4 degree Celsius ov... View More

N-cadherin Antibody in Immunohistochemistry (Paraffin) (IHC (P))

N-cadherin Antibody in Western Blot (WB)

N-cadherin Antibody in Immunohistochemistry (IHC)

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产品信息

PA5-19486

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1 µg/mL

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免疫组化 (IHC)

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免疫组化（石蜡） (IHC (P))

1 µg/mL

免疫组化（冰冻） (IHC (F))

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免疫细胞化学 (ICC/IF)

5 µg/mL

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免疫沉淀 (IP)

Assay-Dependent

产品规格

种属反应

Human, Mouse, Rat

已发表种属

Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Synthetic peptide conjugated to KLH derived from within residues 800 - 900 of Human N Cadherin.

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.7 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS, pH 7.4

内含物

0.02% sodium azide

保存条件

-20°C or -80°C if preferred

运输条件

Ambient (domestic); Wet ice (international)

AB_10979609

产品详细信息

Heat mediated antigen retrieval recommended prior to tissue staining.

This antibody is predicted to react with chicken and cow based on sequence homology.

靶标信息

N-cadherin is a 140 kDa protein belonging to a family of transmembrane molecules that mediate calcium-dependent intercellular adhesion. Cadherins are involved in controlling morphogenetic movements during development and regulate cell surface adhesion through homotypic adhesion with the same cadherin species. N-cadherin's function is dependent on its association with the actin-cytoskeleton and is mediated through interactions between the C-terminal region of N-cadherin and the cytoplasmic catenin proteins. The stability of this association is regulated by phosphorylation and dephosphorylation of beta-catenin. Further, N-cadherin is a calcium dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. The protein functions during gastrulation and is required for establishment of left-right asymmetry.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Overexpression of the telomerase holoenzyme induces EMT and tumorigenesis of HPV-immortalized keratinocytes.

Journal of medical virology

Wang A,Zhou D,Krawczyk E,Li T,Simic V,Lu J,Liu X,Schlegel R,Yuan H

Published figure using N-cadherin polyclonal antibody (Product # PA5-19486) in Western Blot

Sat Apr 01 00:00:00 UTC 2023

N-Cadherin Regulates GluA1-Mediated Depressive-Like Behavior in Adolescent Female Rat Offspring following Prenatal Stress.

Neuroendocrinology

Shao S,Yao D,Li S,Li J,Si Y,Zhang H,Zhu Z,Song D,Li H

PA5-19486 was used in Immunohistochemistry (Frozen) to investigate gender-differences in depression in prenatal stress rat models.

Thu May 05 00:00:00 UTC 2022

Neonatal Hyperoxia Downregulates Claudin-4, Occludin, and ZO-1 Expression in Rat Kidney Accompanied by Impaired Proximal Tubular Development.

Oxidative medicine and cellular longevity

Xu X,Zhang X,Gao L,Liu C,You K

PA5-19486 was used in Immunohistochemistry-immunofluorescence to investigate how neonatal hyperoxia affected the expression of key tight junction proteins and inflammatory factors (IL-6 and TNF-α) in the developing rat kidneys and elucidated their correlation with renal injury.

Tue Sep 07 00:00:00 UTC 2021

H19-Dependent Transcriptional Regulation of β3 and β4 Integrins Upon Estrogen and Hypoxia Favors Metastatic Potential in Prostate Cancer.

International journal of molecular sciences

Bacci L,Aiello A,Ripoli C,Loria R,Pugliese D,Pierconti F,Rotili D,Strigari L,Pinto F,Bassi PF,Mai A,Grassi C,Pontecorvi A,Falcioni R,Farsetti A,Nanni S

PA5-19486 was used in Western Blot to dissect the contribution of long non-coding RNA H19 to PCa metastatic potential under combined stimuli.

Sat Aug 17 00:00:00 UTC 2019

VCAM-1 induces signals that stimulate ZO-1 serine phosphorylation and reduces ZO-1 localization at lung endothelial cell junctions.

Journal of leukocyte biology

Abdala-Valencia H,Kountz TS,Marchese ME,Cook-Mills JM

PA5-19486 was used in Immunohistochemistry to demonstrate that during allergic lung response there was reduced zonula occludens-1 localisation in lung epithelial cell juncyions whereas there was increases lung endothelial cell expression of VCAM-1, N-cadherin and angiomotin.

Sun Jul 01 00:00:00 UTC 2018

ZEB1 Regulates Multiple Oncogenic Components Involved in Uveal Melanoma Progression.

Scientific reports

Chen Y,Lu X,Montoya-Durango DE,Liu YH,Dean KC,Darling DS,Kaplan HJ,Dean DC,Gao L,Liu Y

PA5-19486 was used in Immunocytochemistry-immunoflourescence to study the role of ZEB1 in human uveal melanoma progression.

Fri Mar 03 00:00:00 UTC 2017

Cisplatin promotes mesenchymal-like characteristics in osteosarcoma through Snail.

Oncology letters

Fang S,Yu L,Mei H,Yang J,Gao T,Cheng A,Guo W,Xia K,Liu G

PA5-19486 was used in immunocytochemistry and western blot to test if chemotherapy affects the epithelial-mesenchymal transition of osteosarcoma cells

Thu Dec 01 00:00:00 UTC 2016

Cisplatin promotes mesenchymal-like characteristics in osteosarcoma through Snail.

Oncology letters

Fang S,Yu L,Mei H,Yang J,Gao T,Cheng A,Guo W,Xia K,Liu G

PA5-19486 was used in immunocytochemistry and western blot to test if chemotherapy affects the epithelial-mesenchymal transition of osteosarcoma cells

Thu Dec 01 00:00:00 UTC 2016

生物信息学

蛋白别名: cadherin 2 type 1 N-cadherin (neuronal); cadherin 2, type 1, N-cadherin (neuronal); Cadherin-2; Cadherin-N; Cadherin2; calcium-dependent adhesion protein, neuronal; CD325; CDw325; CDw325 antigen; N-cadherin; N-cadherin 1; Neural cadherin

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基因别名: CD325; CDH2; CDHN; CDw325; N-cadherin; NCAD

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UniProt ID: (Human) P19022, (Mouse) P15116, (Rat) Q9Z1Y3

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Entrez Gene ID: (Human) 1000, (Mouse) 12558, (Rat) 83501

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