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N-terminal Arginylation Antibody (MA5-27563) in WB
Western blot analysis of N-terminal Arginylation in n-terminal arginine-bsa using 0.5 µg of protein. Sample was prepared with 5% milk and TBST, incubated with N-terminal Arginylation monoclonal antibody (Product # MA5-27563) using a dilution of 1:1000 (1 hour at RT) and followed by a Goat Anti-Mouse PE secondary antibody at a dilution of 1:100 (20 min at RT). Samples are arranged as follows: Lane 1: molecul... View More
产品信息
MA5-27563
产品规格
种属反应
Chemical
宿主/亚型
Mouse
/ IgG1
分类
Monoclonal
类型
Antibody
克隆号
4A9
抗原
Synthetic N-terminal arginylated peptide conjugated to KLH
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Protein G
保存液
PBS, pH 7.4, with 50% glycerol
内含物
0.09% sodium azide
保存条件
-20°C
运输条件
Wet ice
RRID
AB_2735284
产品详细信息
MA5-27563 is specific for N-terminal arginine next to glutamic acid. It does not detect N-terminal arginine next to aspartic acid or internal arginine residues.
A 1:1,000 dilution of MA5-27563 was sufficient for detection of N-terminal Arginylation in 0.5 µg of N-terminal Arginine peptide conjugated to BSA by ECL immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.
Specific for N-terminal arginine next to glutamic acid. Does not detect N-terminal arginine next to aspartic acid or internal arginine residues.
靶标信息
Protein arginylation is the post-translational addition of arginine to proteins by arginyltransferase ATE1. Arginylation of proteins has been found to play an essential role in physiological pathways during embryogenesis and adulthood. Arginylation has also been shown to regulate cell stress responses, including ER stress, cytosolic misfolded proteins, and heat stress.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: N terminal Arginine; N-terminal Arginine
