Invitrogen

NEFL Monoclonal Antibody (1B11)

NEFL Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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NEFL Antibody (MA5-47382) in IHC (P)

Chromogenic immunohistochemistry of NEFL in formalin fixed paraffin embedded brain stem section from a transgenic mouse model of ALS. Samples were stained with NEFL monoclonal antibody (Product # MA5-47382) using a dilution of 1:1,000. Detected in DAB (brown) following the Vector LabsM.O.M.® ImmPRESS® HRP method, without the antigen retrieval step. Hematoxylin (blue) was used as the counterstain. The NEFL a... View More

NEFL Antibody in Immunohistochemistry (PFA fixed) (IHC (PFA))

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产品信息

MA5-47382

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:10,000-1:20,000

免疫组化（石蜡） (IHC (P))

1:2,000

免疫组织化学（多聚甲醛固定） (IHC (PFA))

1:2,000

免疫细胞化学 (ICC/IF)

1:2,000

产品规格

种属反应

Bovine, Horse, Human, Mouse, Pig, Rat

宿主/亚型

Mouse / IgG1

分类

Monoclonal

类型

Antibody

克隆号

1B11

抗原

NF-L purified from pig spinal cord

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Protein A/G

保存液

PBS with 50% glycerol

内含物

5mM sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

AB_2942364

靶标信息

Involved in the maintenance of neuronal caliber, neurofilaments are the intermediate filament proteins found specifically in neurons, and are composed predominantly of three major proteins called NF-L, NF-M and NF-H. Like most other intermediate filament proteins (IFPs), the expression of the different neuronal IFPs is both tissue-specific and developmentally regulated. NF-L is the light or low molecular weight microfilament subunit and runs on SDS-PAGE gels at approximately 70 kDa. Neurofilament are the 10nm or intermediate filament proteins found specifically in neurons, and are composed predominantly of three major proteins called NF-L, NF-M and NF-H. NF-H is the heavy or high molecular weight microfilament subunit and runs on SDS-PAGE gels in the range 180-220 kDa, with some variation in different species.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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生物信息学

蛋白别名: 68 kDa neurofilament protein; FLJ53642; light molecular weight neurofilament protein; Micro glutamic acid-rich protein; NEF3; neurofilament; neurofilament 3; Neurofilament light polypeptide; neurofilament protein L; neurofilament protein, light chain; neurofilament subunit NF-L; Neurofilament triplet L protein; neurofilament, light polypeptide; neurofilament, light polypeptide 68kDa; neurofilament-l; neurofilament-light; NF-L; NF-M; NFM; NLC; OTTHUMP00000196614; protein phosphatase 1, regulatory subunit 110

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基因别名: AI847934; CMT1F; CMT2E; NEFL; NF-L; NF68; NFL; PPP1R110