Invitrogen

NEFL Monoclonal Antibody (DA2)

NEFL Antibody in Immunocytochemistry (ICC/IF)

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NEFL Antibody (MA1-2010) in ICC/IF

Immunofluorescence analysis of Neurofilament, Light chain was done on 70% confluent log phase SH-SY5Y cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Neurofilament, Light chain (DA2) Mouse Monoclonal Antibody (Product # MA1-2010) at 2 µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa ... View More

NEFL Antibody in Immunohistochemistry (Paraffin) (IHC (P))

NEFL Antibody in Immunohistochemistry (IHC)

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NEFL Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across tissues tested owing to their inherent genetic constitution. Relative expression of NEFL was observed in PC-12 differentiated to neurons in comparison to PC-12 undifferentiated cells. Relative expression was also observed in Mouse Cerebellum and Mouse Brain in comparison to Mouse Heart and Mouse Lungs using Anti-NEFL Mouse Monoclonal Antibody (Product # MA1-2010) in Western Blot. {RE}

产品信息

MA1-2010

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1-2 µg/mL

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免疫组化 (IHC)

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免疫组化（石蜡） (IHC (P))

Assay-dependent

免疫组化（冰冻） (IHC (F))

Assay-dependent

免疫细胞化学 (ICC/IF)

2-3 µg/mL

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酶联免疫吸附实验 (ELISA)

Assay-dependent

其他PubMed (MISC)

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产品规格

种属反应

Human, Mouse, Pig, Rat

已发表种属

Chicken, Human, Mouse

宿主/亚型

Mouse

分类

Monoclonal

类型

Antibody

克隆号

DA2

抗原

Enzymatically dephosphorylated porcine neurofilaments.

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Protein G

保存液

PBS with 1mg/mL BSA

内含物

0.05% sodium azide

保存条件

-20° C, Avoid Freeze/Thaw Cycles

运输条件

Ambient (domestic); Wet ice (international)

AB_347003

产品详细信息

MA1-2010 detects the neurofilament, light chain in human, rat, and mouse samples.

MA1-2010 has been successfully used in western blot, immunohistochemistry, and immunofluorescence. By western blot this antibody detects a ~70 kDa protein representing the neurofilament, light chain in mouse brain tissue lysate. In immunohistochemistry procedures MA1-2010 recognizes the neurofilament, light chain in mouse brain tissue. In immunofluorescence procedures MA1-2010 recognizes the neurofilament, light chain in rat cerebral cortices.

The MA1-2010 immunogen is enzymatically dephosphorylated porcine neurofilaments.

靶标信息

Involved in the maintenance of neuronal caliber, neurofilaments are the intermediate filament proteins found specifically in neurons, and are composed predominantly of three major proteins called NF-L, NF-M and NF-H. Like most other intermediate filament proteins (IFPs), the expression of the different neuronal IFPs is both tissue-specific and developmentally regulated. NF-L is the light or low molecular weight microfilament subunit and runs on SDS-PAGE gels at approximately 70 kDa. Neurofilament are the 10nm or intermediate filament proteins found specifically in neurons, and are composed predominantly of three major proteins called NF-L, NF-M and NF-H. NF-H is the heavy or high molecular weight microfilament subunit and runs on SDS-PAGE gels in the range 180-220 kDa, with some variation in different species.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

MiR-146a in ALS: Contribution to Early Peripheral Nerve Degeneration and Relevance as Disease Biomarker.

International journal of molecular sciences

Giagnorio E,Malacarne C,Cavalcante P,Scandiffio L,Cattaneo M,Pensato V,Gellera C,Riva N,Quattrini A,Dalla Bella E,Lauria G,Mantegazza R,Bonanno S,Marcuzzo S

Published figure using NEFL monoclonal antibody (Product # MA1-2010) in Western Blot

Mon Feb 27 00:00:00 UTC 2023

Activation of neuronal Ras-related C3 botulinum toxin substrate 1 (Rac1) improves post-stroke recovery and axonal plasticity in mice.

Journal of neurochemistry

Bu F,Munshi Y,Furr JW,Min JW,Qi L,Patrizz A,Spahr ZR,Urayama A,Kofler JK,McCullough LD,Li J

MA1-2010 was used in Immunohistochemistry to identify a neuronal Rac1 signaling in improving functional recovery and axonal regeneration after stroke, suggesting a potential therapeutic target in the recovery stage of stroke.

Sat May 01 00:00:00 UTC 2021

Increased plasma neurofilament light chain concentration correlates with severity of post-mortem neurofibrillary tangle pathology and neurodegeneration.

Acta neuropathologica communications

Ashton NJ,Leuzy A,Lim YM,Troakes C,Hortobágyi T,Höglund K,Aarsland D,Lovestone S,Schöll M,Blennow K,Zetterberg H,Hye A

MA1-2010 was used in Immunohistochemistry to examine the connection between blood neurofilament light chain and brain tissue pathology.

Wed Jan 09 00:00:00 UTC 2019

Increased plasma neurofilament light chain concentration correlates with severity of post-mortem neurofibrillary tangle pathology and neurodegeneration.

Acta neuropathologica communications

Ashton NJ,Leuzy A,Lim YM,Troakes C,Hortobágyi T,Höglund K,Aarsland D,Lovestone S,Schöll M,Blennow K,Zetterberg H,Hye A

MA1-2010 was used in Immunohistochemistry to examine the connection between blood neurofilament light chain and brain tissue pathology.

Wed Jan 09 00:00:00 UTC 2019

Microbiome-Derived Lipopolysaccharide (LPS) Selectively Inhibits Neurofilament Light Chain (NF-L) Gene Expression in Human Neuronal-Glial (HNG) Cells in Primary Culture.

Frontiers in neuroscience

Lukiw WJ,Cong L,Jaber V,Zhao Y

MA1-2010 was used in Western Blotting to characterise a neuron-specific effect of a human gastrointestinal-tract microbiome-derived neurotoxin on decreased neurofilament light abundance in both sporadic Alzheimer's disease temporal lobe neocortex in vivo and in lipopolysaccharide-stressed HNG cells in vitro.

Mon Apr 08 00:00:00 UTC 2024

Microbiome-Derived Lipopolysaccharide (LPS) Selectively Inhibits Neurofilament Light Chain (NF-L) Gene Expression in Human Neuronal-Glial (HNG) Cells in Primary Culture.

Frontiers in neuroscience

Lukiw WJ,Cong L,Jaber V,Zhao Y

Mon Apr 08 00:00:00 UTC 2024

Phagocytosis of neuronal debris by microglia is associated with neuronal damage in multiple sclerosis.

Glia

Huizinga R,van der Star BJ,Kipp M,Jong R,Gerritsen W,Clarner T,Puentes F,Dijkstra CD,van der Valk P,Amor S

MA1-2010 was used in immunohistochemistry to investigate phagocytosis of neuronal antigens in a multiple sclerosis model.

Thu Mar 01 00:00:00 UTC 2012

Alphab-crystallin is a target for adaptive immune responses and a trigger of innate responses in preactive multiple sclerosis lesions.

Journal of neuropathology and experimental neurology

van Noort JM,Bsibsi M,Gerritsen WH,van der Valk P,Bajramovic JJ,Steinman L,Amor S

MA1-2010 was used in western blot to compare the serum immunoglobulin G reactivity profiles obtained using the full spectrum of human myelin-associated proteins of multiple sclerosis patients and healthy control subjects.

Thu Jul 01 00:00:00 UTC 2010

Numerous conglomerate inclusions in slowly progressive familial amyotrophic lateral sclerosis with posterior column involvement.

Journal of the neurological sciences

Katayama S,Watanabe C,Noda K,Ohishi H,Yamamura Y,Nishisaka T,Inai K,Asayama K,Murayama S,Nakamura S

MA1-2010 was used in immunohistochemistry (paraffin) to characterize disease in a patient with familial amyotrophic lateral sclerosis.

Wed Dec 01 00:00:00 UTC 1999

Identification of a tektin-like protein associated with neurofilaments in the developing chick nervous system.

Journal of neuroscience research

Edson KJ,Linck RW,Letourneau PC

MA1-2010 was used in immunocytochemistry to investigate the association between tektin-like protein and neurofilaments in chicken neurons

Sun Sep 01 00:00:00 UTC 1991

生物信息学

蛋白别名: 68 kDa neurofilament protein; FLJ53642; light molecular weight neurofilament protein; NEF3; neurofilament; neurofilament 3; Neurofilament light polypeptide; neurofilament protein L; neurofilament protein, light chain; neurofilament subunit NF-L; Neurofilament triplet L protein; neurofilament, light polypeptide 68kDa; neurofilament-l; neurofilament-light; NF-L; NF-M; NFM; NLC; OTTHUMP00000196614; protein phosphatase 1, regulatory subunit 110

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基因别名: AI847934; CMT1F; CMT2E; NEFL; NF-L; NF68; NFL; PPP1R110

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UniProt ID: (Human) P07196, (Mouse) P08551, (Rat) P19527

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Entrez Gene ID: (Human) 4747, (Pig) 100521224, (Mouse) 18039, (Rat) 83613

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