Invitrogen

NEK2 Polyclonal Antibody

NEK2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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NEK2 Antibody (PA5-31259) in IHC (P)

NEK2 Polyclonal Antibody detects NEK2 protein at cytoplasm and nucleus on human ovarian carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human ovarian carcinoma. NEK2 Polyclonal Antibody (Product # PA5-31259) diluted at 1:250. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.

Product Image Gallery

NEK2 Antibody — Figure 3 NEK2 silencing reduces GSC self-renewal in vitro and tumorigenesis in vivo. (A) Western blotting for NEK2 in 528 glioma spheres transduced with shNEK2 no. 1, shNEK2 no. 2, or shNT. (B) In vitro growth assay showed that NEK2 silencing inhibited the cell proliferation of 528 glioma spheres (n = 6). ***P < 0.001, by 1-way ANOVA. (C) An in vitro clonogenicity assay by limiting dilution neurosphere formation indicated that NEK2 silencing decreased the clonogenicity of 528 glioma spheres (n = 10). ***P < 0.001, by ELDA. (D) qRT-PCR for CD133 expression in 528 glioma spheres transduced with shNEK2 no. 1, shNEK2 no. 2, or shNT (n = 3). *P < 0.05 and ***P < 0.001, by 1-way ANOVA followed by Dunnett’s post-hoc test. (E) ICC for NEK2 and GFAP expression in 528 glioma spheres transduced with shNEK2 no. 1, shNEK2 no. 2, or shNT. Scale bars: 5 µm. (F) Representative images of H&E-stained mouse brain sections after the intracranial transplantation of 528 glioma spheres transduced with shNEK2 no. 1, shNEK2 no. 2, or shNT. Scale bars: 1 mm (top) and 100 µm (bottom). (G) Kaplan-Meier analysis of nude mice harboring an intracranial tumor derived from 528 glioma spheres transduced with shNT (n = 6), shNEK2 no. 1 (n = 5), or shNEK2 no. 2 (n = 5). **P = 0.0021, for shNT versus shNEK2 no. 1; **P = 0.0017, for shNT versus shNEK2 no. 2; both by log-rank test. {KD}

产品信息

PA5-31259

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:3,000

免疫组化（石蜡） (IHC (P))

1:100-1:1,000

免疫细胞化学 (ICC/IF)

1:100-1:1,000

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产品规格

种属反应

Human

已发表种属

Human

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Recombinant protein fragment corresponding to a region within amino acids 185 and 349 of Human NEK2

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS, pH 7, with 1% BSA, 20% glycerol

内含物

0.01% thimerosal

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

AB_2548733

产品详细信息

Recommended positive controls: 293T.

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

靶标信息

NEK2 is involved in cell cycle regulation and is essential for faithful chromosome segregation. NEK2 is a gene that encodes for a serine/threonine-protein kinase. This protein plays a crucial role in the regulation of mitosis and is primarily located in the centrosome. During the G1 phase, the protein is undetectable, but its levels progressively increase throughout the S phase, reaching their peak in late G2 phase. The gene has several alternatively spliced transcript variants that encode different isoforms with distinct C-termini. NEK2 is associated with several diseases, including Retinitis Pigmentosa 67 and Retinitis Pigmentosa.

⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

NEK2 inhibition triggers anti-pancreatic cancer immunity by targeting PD-L1.

Nature communications

Zhang X,Huang X,Xu J,Li E,Lao M,Tang T,Zhang G,Guo C,Zhang X,Chen W,Yadav DK,Bai X,Liang T

PA5-31259 was used in Immunocytochemistry to propose a promising strategy for improving the effectiveness of pancreatic cancer immunotherapy.

Tue Jul 27 00:00:00 UTC 2021

Targeting NEK2 attenuates glioblastoma growth and radioresistance by destabilizing histone methyltransferase EZH2.

The Journal of clinical investigation

Wang J,Cheng P,Pavlyukov MS,Yu H,Zhang Z,Kim SH,Minata M,Mohyeldin A,Xie W,Chen D,Goidts V,Frett B,Hu W,Li H,Shin YJ,Lee Y,Nam DH,Kornblum HI,Wang M,Nakano I

PA5-31259 was used in Immunocytochemistry to demonstrate a key role for NEK2 in maintaining GSCs in GBM by stabilizing the EZH2 protein and introduce the small-molecule inhibitor CMP3a as a potential therapeutic agent for GBM.

Tue Aug 01 00:00:00 UTC 2017

生物信息学

蛋白别名: HSPK 21; NEK 2; NEK 2A; Never in mitosis A-related kinase 2; NIMA (never in mitosis gene a)-related kinase 2; NimA-like protein kinase 1; NIMA-related kinase 2; NimA-related protein kinase 2; NLK 1; protein phosphatase 1, regulatory subunit 111; Serine/threonine-protein kinase Nek2

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基因别名: HsPK21; NEK2; NEK2A; NLK1; PPP1R111; RP67

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UniProt ID: (Human) P51955

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Entrez Gene ID: (Human) 4751

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功能

protein kinase activity protein serine/threonine kinase activity protein binding ATP binding protein phosphatase binding metal ion binding non-receptor serine/threonine protein kinase protein modifying enzyme

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