Invitrogen

NOP14 Polyclonal Antibody

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高级验证 (1)

NOP14 Antibody in Immunocytochemistry (ICC/IF)

NOP14 Antibody in Immunocytochemistry (ICC/IF)

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NOP14 Antibody (PA5-58851) in ICC/IF

Immunofluorecent analysis of NOP14 in human cell line U-2 OS using NOP14 Polyclonal Antibody (Product # PA5-58851). Staining shows localization to nucleus and nucleoli.

NOP14 Antibody in Immunocytochemistry (ICC/IF)

NOP14 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

NOP14 Antibody in Western Blot (WB)

NOP14 Antibody in Western Blot (WB)

Product Image Gallery

NOP14 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorecent analysis of NOP14 in human cell line U-2 OS using NOP14 Polyclonal Antibody (Product # PA5-58851). Staining shows localization to nucleus and nucleoli.

NOP14 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical analysis of NOP14 in human hippocampus using NOP14 Polyclonal Antibody (Product # PA5-58851) shows moderate nucleolar positivity in neurons.

NOP14 Antibody in Western Blot (WB) — Western blot analysis of NOP14 in mouse cell line NIH-3T3 and rat cell line NBT-II using a NOP14 Polyclonal Antibody (Product # PA5-58851).

NOP14 Antibody in Western Blot (WB) — Western blot analysis of NOP14 in U-251MG cells transfected with control siRNA, target specific siRNA probe #1 and #2, using a NOP14 Polyclonal Antibody (Product # PA5-58851). Remaining relative intensity is presented. Loading control: Anti-GAPDH.

NOP14 Antibody — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. U-251 mg cells were transfected with, control siRNA, NOP14 siRNA probe #1, and NOP14 siRNA probe #2 and a decrease in signal intensity was observed in Western Blot application using Anti-NOP14 Polyclonal Antibody (Product # PA5-58851) used at a dilution of 0.4 µg/mL. Anti-GAPDH was used as a loading control. {KD}

产品信息

PA5-58851

应用

建议稀释比

已发表文章

免疫印迹 (WB)

0.04-0.4 µg/mL

-

免疫组化（石蜡） (IHC (P))

1:500-1:1,000

-

免疫细胞化学 (ICC/IF)

0.25-2 µg/mL

-

产品规格

种属反应

Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Recombinant protein corresponding to Human NOP14. Recombinant protein control fragment (Product #RP-96613).

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.1 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS, pH 7.2, with 40% glycerol

内含物

0.02% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

RRID

AB_2644739

产品详细信息

Immunogen sequence: REKPKSRKEL IEELIAKSKQ EKRERQAQRE DALELTEKLD QDWKEIQTLL SHKTPKSENR DKKEKPKPDA YDMMVRELGF EMKAQPSNRM KT

Highest antigen sequence identity to the following orthologs: Mouse - 86%, Rat - 89%.

靶标信息

Involved in nucleolar processing of pre-18S ribosomal RNA. Has a role in the nuclear export of 40S pre-ribosomal subunit to the cytoplasm.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: NOP14 nucleolar protein homolog; Nucleolar complex protein 14; Nucleolar protein 14; probable nucleolar complex protein 14

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基因别名: 2610033H07Rik; C4orf9; NOL14; NOP14; RES4-25; RES425; RGD1305605; UTP2

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UniProt ID: (Human) P78316, (Mouse) Q8R3N1

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Entrez Gene ID: (Human) 8602, (Mouse) 75416, (Rat) 289724

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enzyme binding snoRNA binding poly(A) RNA binding RNA metabolism protein nucleic acid metabolism protein

参与通路

endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) endonucleolytic cleavage to generate mature 5'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA) endonucleolytic cleavage in 5'-ETS of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) rRNA processing maturation of SSU-rRNA ribosomal small subunit biogenesis biological_process ribosome biogenesis