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NeuroD1 Antibody (MA5-32626) in ICC/IF
Immunofluorescence analysis of NeuroD1 was performed using 70% confluent log phase IMR-32 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with NeuroD1 Recombinant Rabbit Monoclonal Antibody (JM11-10) (Product # MA5-32626) at 5 µg/mL in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Goat anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluo... View More
产品信息
MA5-32626
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse,
Rat
宿主/亚型
Rabbit
/ IgG
Expression System
HEK293 cells
分类
Recombinant Monoclonal
类型
Antibody
克隆号
JM11-10
抗原
Synthetic peptide within Human NeuroD1 aa 1-44
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Protein A
保存液
TBS, pH 7.4, with 40% Glycerol, 0.05% BSA
内含物
0.05% sodium azide
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Wet ice
RRID
AB_2809903
产品详细信息
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
靶标信息
NeuroD1 is a transcriptional activator that acts as a differentiation factor during neurogenesis. It has been demonstrated to bind to the insulin gene E-box. Efficient DNA binding requires dimerization with another basic helix-loop-helix (bHLH) protein. Defects in NEUROD1 are a cause of maturity onset diabetes of the young type VI (MODY6). MODY6 is a form of non-insulin-dependent diabetes mellitus characterized by an autosomal dominant mode of inheritance, onset during young adulthood and a primary defect in insulin secretion.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: Basic helix-loop-helix factor 1; basic helix-loop-helix transcription factor; beta-cell E-box transactivator 2; Beta-cell E-box transcriptional activator 2; Beta2; BHF-1; BHF1; bHLHa3; Class A basic helix-loop-helix protein 3; neuro d1; NeuroD; NeuroD1; neurogenic differentiation 1; Neurogenic differentiation factor 1; neurogenic helix-loop-helix protein NEUROD
基因别名: BETA2; BHF-1; BHLHA3; MODY6; NEUROD; NEUROD1
UniProt ID: (Human) Q13562, (Rat) Q64289, (Mouse) Q60867
Entrez Gene ID: (Human) 4760, (Rat) 29458, (Mouse) 18012
RNA polymerase II core promoter proximal region sequence-specific DNA binding
transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding
RNA polymerase II activating transcription factor binding
RNA polymerase II transcription coactivator activity
chromatin binding
transcription factor activity, sequence-specific DNA binding
transcription coactivator activity
protein binding
transcription factor binding
sequence-specific DNA binding
protein heterodimerization activity
E-box binding
DNA binding
double-stranded DNA binding
protein dimerization activity
basic helix-loop-helix transcription factor
pancreatic A cell fate commitment
pancreatic PP cell fate commitment
transcription from RNA polymerase II promoter
nucleocytoplasmic transport
nitric oxide mediated signal transduction
response to glucose
anterior/posterior pattern specification
dentate gyrus development
cerebellum development
neurogenesis
signal transduction involved in regulation of gene expression
insulin secretion
endocrine pancreas development
amacrine cell differentiation
enteroendocrine cell differentiation
response to drug
glucose homeostasis
positive regulation of apoptotic process
positive regulation of cell differentiation
positive regulation of neuron differentiation
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
negative regulation of JAK-STAT cascade
embryonic organ morphogenesis
neuron development
inner ear development
regulation of insulin secretion
positive regulation of sequence-specific DNA binding transcription factor activity
regulation of intestinal epithelial structure maintenance
regulation of cell cycle arrest
cellular response to glucose stimulus
negative regulation of type B pancreatic cell apoptotic process
positive regulation of transcription regulatory region DNA binding
transcription, DNA-templated
hindbrain development
camera-type eye development
cell fate commitment
regulation of neuron differentiation
regulation of transcription, DNA-templated
multicellular organism development
nervous system development
cell differentiation
