Invitrogen

Neurofascin Polyclonal Antibody

Neurofascin Antibody in Immunocytochemistry (ICC/IF)

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Neurofascin Antibody (PA5-78668) in ICC/IF

Neurofascin Polyclonal Antibody detects Neurofascin protein by immunofluorescent analysis. Sample: DIV14 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min. Green: Neurofascin protein stained by Neurofascin Polyclonal Antibody (Product # PA5-78668) diluted at 1:500. Red: beta Tubulin 3/ Tuj1, stained by beta-3 Tubulin Polyclonal Antibody [GT1338]diluted at 1:500. Blue: Fluor... View More

Neurofascin Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Neurofascin Antibody in Immunohistochemistry (Frozen) (IHC (F))

Neurofascin Antibody in Western Blot (WB)

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产品信息

PA5-78668

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:3,000

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免疫组化（石蜡） (IHC (P))

1:100-1:1,000

免疫组化（冰冻） (IHC (F))

1:100-1:1,000

免疫细胞化学 (ICC/IF)

1:100-1:1,000

免疫沉淀 (IP)

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产品规格

种属反应

Mouse, Rat

已发表种属

Human

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of mouse Neurofascin. The exact sequence is proprietary.

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.14 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS, pH 7, with 20% glycerol, 1% BSA

内含物

0.025% ProClin 300

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

AB_2736209

产品详细信息

Positive Control: mouse brain, rat brain

Predicted Reactivity: Human (100%)

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

靶标信息

Neurofascin encodes an L1 family immunoglobulin cell adhesion molecule with multiple IGcam and fibronectin domains. The protein functions in neurite outgrowth, neurite fasciculation, and organization of the axon initial segment (AIS) and nodes of Ranvier on axons during early development. Both the AIS and nodes of Ranvier contain high densities of voltage-gated Na+ (Nav) channels which are clustered by interactions with cytoskeletal and scaffolding proteins including this protein, gliomedin, ankyrin 3 (ankyrin-G), and betaIV spectrin. This protein links the AIS extracellular matrix to the intracellular cytoskeleton. Neurofascin undergoes extensive alternative splicing, and the full-length nature of some variants has not been determined.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Multi-omic profiling reveals the ataxia protein sacsin is required for integrin trafficking and synaptic organization.

Cell reports

Romano LEL,Aw WY,Hixson KM,Novoselova TV,Havener TM,Howell S,Taylor-Blake B,Hall CL,Xing L,Beri J,Nethisinghe S,Perna L,Hatimy A,Altadonna GC,Graves LM,Herring LE,Hickey AJ,Thalassinos K,Chapple JP,Wolter JM

PA5-78668 was used in Immunocytochemistry, Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin), Western Blot, Immunoprecipitation to suggest that disrupted trafficking of synaptic adhesion proteins is a causal molecular deficit in ARSACS.