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Neuroligin 1 (extracellular) Polyclonal Antibody

查看其他19个Neuroligin 1抗体

抗体检测数据 (4)

Neuroligin 1 (extracellular) Antibody in Immunocytochemistry (ICC/IF)

Neuroligin 1 (extracellular) Antibody in Immunocytochemistry (ICC/IF)

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Neuroligin 1 (extracellular) Antibody (ANR-035-200UL) in ICC/IF

Expression of NLGN1 in rat PC12 cells - Cell surface detection of NLGN1 in intact living rat pheochromocytoma PC12 cells. A. Extracellular staining of cells using Anti-Neuroligin 1 (extracellular) Antibody (#ANR-035), (1:100) followed by Anti-rabbit-AlexaFluor-594 secondary Antibody (red). B. Merge of staining with live view of the cells.

Neuroligin 1 (extracellular) Antibody in Immunocytochemistry (ICC/IF)

Neuroligin 1 (extracellular) Antibody in Immunohistochemistry (Frozen) (IHC (F))

Neuroligin 1 (extracellular) Antibody in Immunohistochemistry (Frozen) (IHC (F))

Neuroligin 1 (extracellular) Antibody in Western Blot (WB)

Product Image Gallery

Neuroligin 1 (extracellular) Antibody in Immunocytochemistry (ICC/IF) — Expression of NLGN1 in rat PC12 cells - Cell surface detection of NLGN1 in intact living rat pheochromocytoma PC12 cells. A. Extracellular staining of cells using Anti-Neuroligin 1 (extracellular) Antibody (#ANR-035), (1:100) followed by Anti-rabbit-AlexaFluor-594 secondary Antibody (red). B. Merge of staining with live view of the cells.

Neuroligin 1 (extracellular) Antibody in Immunohistochemistry (Frozen) (IHC (F)) — Expression of NLGN1 in rat DRG - Immunohistochemical staining of adult rat dorsal root ganglion (DRG) using Anti-Neuroligin 1 (extracellular) Antibody (#ANR-035) followed by Anti-rabbit-AlexaFluor-594 secondary Antibody . A. NLGN1 labeling (red) appears in the cell bodies of the DRG neurons. B. Nuclear staining using DAPI as the counter stain (blue). C. Merged image of A and B.

Neuroligin 1 (extracellular) Antibody in Immunohistochemistry (Frozen) (IHC (F)) — Expression of NLGN1 in rat hippocampus and cortex - Immunohistochemical staining of free floating frozen section of rat hippocampal CA1 region (A, B) and rat parietal cortex (C, D) using Anti-Neuroligin 1 (extracellular) Antibody (#ANR-035), (1:300). A. NLGN1 staining (green) appears in several neuronal profiles in the pyramidal layer (arrows). C. NLGN1 staining (green) is detected in several neuronal profiles including pyramidal cells and their apical dendrite (arrows). B, D. Nuclei are stained using DAPI as the counterstain (blue).

Neuroligin 1 (extracellular) Antibody in Western Blot (WB) — Western blot analysis of mouse (lanes 1 and 3) and rat (lanes 2 and 4)brain lysates: - 1,2. Anti-Neuroligin 1 (extracellular) Antibody (#ANR-035), (1:800).3,4. Anti-Neuroligin 1 (extracellular) Antibody preincubated with Neuroligin 1 (extracellular) Blocking Peptide (#BLP-NR035).

产品信息

ANR-035-200UL

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:800

-

免疫组化（冰冻） (IHC (F))

1:300

-

免疫细胞化学 (ICC/IF)

1:100

-

产品规格

种属反应

Mouse, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

(C)KVPSTDITLRPTRK, corresponding to amino acid residues 648-661 of rat NLGN1, Extracellular, N-terminus

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Lyophilized

浓度

0.8 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS, pH 7.4, with 1% BSA

内含物

0.05% sodium azide

保存条件

-20° C, Avoid Freeze/Thaw Cycles

运输条件

Ambient (domestic); Wet ice (international)

产品详细信息

Reconstitution: 25 µL, 50 µL or 0.2 mL double distilled water (DDW), depending on the sample size. The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20C. The reconstituted solution can be stored at 4C for up to 1 week. For longer periods, small aliquots should be stored at -20C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

靶标信息

This gene encodes a member of a family of neuronal cell surface proteins. Members of this family may act as splice site-specific ligands for beta-neurexins and may be involved in the formation and remodeling of central nervous system synapses.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: MGC45115; Neuroligin I; Neuroligin-1; NLG 1

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基因别名: 6330415N05Rik; BB179718; Kiaa1070; mKIAA1070; NL1; Nlg1; Nlgn1

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UniProt ID: (Mouse) Q99K10, (Rat) Q62765

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Entrez Gene ID: (Mouse) 192167, (Rat) 116647

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receptor activity protein binding PDZ domain binding neurexin family protein binding protein dimerization activity cell adhesion molecule binding carboxylic ester hydrolase activity scaffold protein binding cell adhesion molecule

regulation of respiratory gaseous exchange by neurological system process protein targeting cell adhesion heterophilic cell-cell adhesion via plasma membrane cell adhesion molecules neuron cell-cell adhesion nervous system development synapse assembly positive regulation of circadian sleep/wake cycle, wakefulness synaptic vesicle targeting calcium-dependent cell-cell adhesion via plasma membrane cell adhesion molecules neuron projection development positive regulation of synaptic transmission, GABAergic protein localization to synapse establishment of protein localization regulation of neuron differentiation synaptic vesicle transport rhythmic process cytoskeletal matrix organization at active zone modulation of synaptic transmission synapse organization protein homooligomerization protein heterotetramerization positive regulation of filopodium assembly positive regulation of synapse assembly positive regulation of synaptic transmission, glutamatergic inhibitory postsynaptic potential long-term synaptic potentiation positive regulation of dendritic spine development negative regulation of dendritic spine morphogenesis terminal button organization synaptic vesicle clustering postsynaptic membrane assembly presynaptic membrane assembly AMPA glutamate receptor clustering NMDA glutamate receptor clustering neurexin clustering involved in presynaptic membrane assembly postsynaptic density protein 95 clustering receptor localization to synapse positive regulation of ruffle assembly positive regulation of synaptic vesicle endocytosis positive regulation of protein localization to synapse positive regulation of intracellular signal transduction positive regulation of synaptic vesicle exocytosis regulation of N-methyl-D-aspartate selective glutamate receptor activity regulation of alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionate selective glutamate receptor activity positive regulation of excitatory postsynaptic potential positive regulation of synaptic vesicle clustering metabolic process neuronal ion channel clustering