Invitrogen

Noggin Polyclonal Antibody

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抗体检测数据 (2)

Noggin Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Noggin Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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Noggin Antibody (PA5-95474) in IHC (P)

Immunohistochemistry analysis of Noggin in paraffin-embedded section of human placenta tissue. Samples were incubated with Noggin polyclonal antibody (Product # PA5-95474) using a dilution of 1 μg/mL overnight at 4°C followed by Peroxidase Conjugated Goat Anti-rabbit IgG incubated for 30 minutes at 37°C. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The t... View More

Noggin Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Noggin Antibody in Western Blot (WB)

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Noggin Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of Noggin in paraffin-embedded section of human placenta tissue. Samples were incubated with Noggin polyclonal antibody (Product # PA5-95474) using a dilution of 1 μg/mL overnight at 4°C followed by Peroxidase Conjugated Goat Anti-rabbit IgG incubated for 30 minutes at 37°C. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.

Noggin Antibody in Western Blot (WB) — Western blot analysis of Noggin in Lane 1: human HeLa whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human A375 whole cell lysates, Lane 5: human 22RV1 whole cell lysates. Electrophoresis was performed with 5-20% SDS-PAGE gel (70V, Stacking gel; 90V Resolving gel, Time: 2-3 hours), transferred to a nitrocellulose membrane and blocked using 5% Non-fat Milk/TBS (1.5 hrs at room temperature). Samples were incubated with Noggin polyclonal antibody (Product # PA5-95474) using a 0.5 µg/mL dilution, followed by a goat anti-rabbit IgG-HRP at a dilution of 1:10,000, and developed with enhanced chemiluminescence (ECL).

产品信息

PA5-95474

应用

建议稀释比

已发表文章

免疫印迹 (WB)

0.1-0.5 µg/mL

-

免疫组化（石蜡） (IHC (P))

0.5-1 µg/mL

-

酶联免疫吸附实验 (ELISA)

0.1-0.5 µg/mL

-

产品规格

种属反应

Human

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

E.coli-derived human Noggin recombinant protein (Position: L43-T153).

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Lyophilized

浓度

500 µg/mL

纯化类型

Affinity chromatography

保存液

PBS with 4mg trehalose

内含物

0.01mg sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

AB_2807276

产品详细信息

Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.

靶标信息

Noggin is involved in numerous developmental processes, such as neural tube fusion and joint formation. The morphogenesis of organs is initiated by a downgrowth from a layer of epithelial stem cells. This process is achieved through the receipt of signals from 1) a WNT protein (WNT3A) to stabilize beta-catenin; and 2) Noggin, which is a bone morphogenetic protein inhibitor. Noggin mutations in unrelated families with proximal symphalangism (SYM1) and multiple synostoses syndrome (SYNS1) have been identified, which have multiple joint fusion as their principal defect.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: Noggin; Noggin precursor; symphalangism 1 (proximal)

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基因别名: NOG; SYM1; SYNS1; SYNS1A

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UniProt ID: (Human) Q13253

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Entrez Gene ID: (Human) 9241

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protein binding cytokine binding protein complex binding protein homodimerization activity intercellular signal molecule

negative regulation of transcription from RNA polymerase II promoter skeletal system development osteoblast differentiation in utero embryonic development endoderm formation mesoderm formation epithelial to mesenchymal transition neural plate morphogenesis neural tube closure nervous system development motor neuron axon guidance dorsal/ventral pattern formation spinal cord development cell differentiation in hindbrain pituitary gland development negative regulation of cell migration BMP signaling pathway negative regulation of BMP signaling pathway somatic stem cell population maintenance wound healing middle ear morphogenesis embryonic digit morphogenesis regulation of neuron differentiation negative regulation of osteoblast differentiation positive regulation of transcription from RNA polymerase II promoter axial mesoderm development notochord morphogenesis embryonic skeletal system development negative regulation of astrocyte differentiation positive regulation of epithelial cell proliferation cartilage development negative regulation of cardiac muscle cell proliferation limb development embryonic skeletal joint morphogenesis negative regulation of cytokine activity face morphogenesis negative regulation of pathway-restricted SMAD protein phosphorylation lung morphogenesis prostatic bud formation ureteric bud formation fibroblast growth factor receptor signaling pathway involved in neural plate anterior/posterior pattern formation negative regulation of cartilage development somite development negative regulation of canonical Wnt signaling pathway positive regulation of branching involved in ureteric bud morphogenesis positive regulation of glomerulus development regulation of fibroblast growth factor receptor signaling pathway involved in neural plate anterior/posterior pattern formation negative regulation of apoptotic signaling pathway