Invitrogen

P-Cadherin Monoclonal Antibody (CSTEM29), eBioscience™

P-Cadherin Antibody in Immunocytochemistry (ICC/IF)

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P-Cadherin Antibody (14-2237-82) in ICC/IF

Immunofluorescence analysis of Cadherin-3 was performed using 70% confluent log phase A-431 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.01% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with P-Cadherin Monoclonal Antibody (CSTEM29), eBioscience™ (Product # 14-2237-82) at 5µg/mL in 0.1% BSA, incub... View More

P-Cadherin Antibody in Western Blot (WB)

P-Cadherin Antibody in Flow Cytometry (Flow)

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产品信息

14-2237-82

应用

建议稀释比

已发表文章

免疫印迹 (WB)

10 µg/mL

免疫细胞化学 (ICC/IF)

5 µg/mL

流式细胞分析 (Flow)

0.25 µg/test

产品规格

种属反应

Human, Mouse

宿主/亚型

Mouse / IgG2a, kappa

分类

Monoclonal

类型

Antibody

克隆号

CSTEM29

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2

内含物

0.09% sodium azide

保存条件

4° C, do not freeze

运输条件

Ambient (domestic); Wet ice (international)

AB_2688283

产品详细信息

This monoclonal antibody CSTEM29 reacts with human P-Cadherin, also known as Cadherin-3.

Applications Reported: This CSTEM29 antibody has been reported for use in flow cytometric analysis and immunoblot.

Applications Tested: This CSTEM29 antibody has been tested by flow cytometric analysis of normal human induced pluripotent stem cells, and for western blot. For flow cytometry, this may be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. For western blot, this can be used at less than or equal to 10 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

P-cadherin, also known as Cadherin-3, is a Type 1 cadherin protein that belongs to the cadherin superfamily. Type 1 cadherins are single-pass transmembrane proteins that have 5 extracellular cadherin repeats and an intracellular domain that binds p120-catenin and beta-catenin. Cadherins are calcium-dependent cell-cell adhesion glycoproteins responsible for a range of processes including development, wound healing, cell-cell signaling, cell growth and differentiation. P-cadherin is expressed in human fetal structures, and in adult tissues such as the basal layer of the epidermis, breast, prostate, ovary, cervix, hair follicle, and corneal endothelium. P-cadherin expression has also been reported on embryonic stem cells, and stem cells of the normal mammary gland, and hair follicle. Overexpression of P-cadherin has been associated with poor prognosis in breast, prostate, ovary, colon, and stomach carcinomas. Mutations in CDH3 are associated with congential hypotrichosis with juvenile macular dystrophy.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: CADH3; cadherin 3, type 1, P-cadherin (placental); Cadherin-3; calcium-dependent adhesion protein, placental; P-cadherin; Placental cadherin; Placental-cadherin; RPE-specific cadherin

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基因别名: AI385538; Cadp; CDH3; CDHP; HJMD; P-cadherin; PCAD

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UniProt ID: (Human) P22223, (Mouse) P10287

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Entrez Gene ID: (Human) 1001, (Mouse) 12560

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功能

molecular_function calcium ion binding metal ion binding cadherin

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