Invitrogen

PARP1 (cleaved Asp214, Asp215) Polyclonal Antibody

PARP1 (cleaved Asp214, Asp215) Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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PARP1 (cleaved Asp214, Asp215) Antibody (44-698G) in IHC (P)

PARP (214⁄215) cleavage site specific antibody. Image shows immunohistochemical staining of cleaved PARP in formalin-fixed, paraffin embedded human tonsillar tissue. Antigen retrieval done using citrate buffer.

PARP1 (cleaved Asp214, Asp215) Antibody in Western Blot (WB)

PARP1 (cleaved Asp214, Asp215) Antibody in Immunohistochemistry, Immunohistochemistry (Paraffin) (IHC, IHC (P))

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产品信息

44-698G

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000

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免疫组化 (IHC)

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免疫组化（石蜡） (IHC (P))

Assay-dependent

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免疫细胞化学 (ICC/IF)

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流式细胞分析 (Flow)

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其他PubMed (Misc)

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产品规格

种属反应

Bovine, Human, Mouse, Rat

已发表种属

Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

The antiserum was produced against a chemically synthesized peptide corresponding to the N-terminus of cleavage site (214/215) of human PARP.

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

纯化类型

Antigen affinity chromatography

保存液

Dulbecco's PBS, pH 7.3, with 1mg/mL BSA

内含物

0.05% sodium azide

保存条件

-20°C

运输条件

Ambient (domestic); Wet ice (international)

AB_2533726

产品详细信息

This antibody specifically recognizes the 85 kDa fragment of cleaved PARP and can be used as a marker for detecting apoptotic cells. The antibody has been used in western blotting and previous lots have been used in immunostaining applications. Positive controls used in western blotting were Jurkat or HeLa cells treated with staurosporine or etoposide (25 µM for 3 hours).

靶标信息

Poly ADP-Ribose Polymerase (PARP) uses nicotinamide adenine dinucleotide (oxidized form) NAD as a substrate to catalyse the transfer of ADP-ribose to a variety of nuclear protein acceptors. Proteolysis of PARP to its stable 85kDa fragment is an early marker of programmed cell death (apoptosis) and is mediated by the caspase CPP32 protein. Cleavage occurs between Adp216 and Gly217, a site in PARP conserved across species.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Penta-O-Galloyl-β-D-Glucose in Pistacia integerrima Targets AMPK-ULK1 and ERK/STAT3 Signaling Axes to Induce ROS-Independent Autophagic Cell Death in Human Lung Cancer Cells.

Frontiers in pharmacology

Balkrishna A,Mulay VP,Verma S,Srivastava J,Lochab S,Varshney A

44-698G was used in Western Blotting to propose PGG as a potential AMPK activator and STAT3 inhibitor that can be exploited further in developing adjuvant chemotherapeutics against lung cancer.

Sat Aug 06 00:00:00 UTC 2022

Penta-O-Galloyl-β-D-Glucose in Pistacia integerrima Targets AMPK-ULK1 and ERK/STAT3 Signaling Axes to Induce ROS-Independent Autophagic Cell Death in Human Lung Cancer Cells.

Frontiers in pharmacology

Balkrishna A,Mulay VP,Verma S,Srivastava J,Lochab S,Varshney A

44-698G was used in Western Blotting to propose PGG as a potential AMPK activator and STAT3 inhibitor that can be exploited further in developing adjuvant chemotherapeutics against lung cancer.

Sat Aug 06 00:00:00 UTC 2022

Adrenomedullin-CALCRL axis controls relapse-initiating drug tolerant acute myeloid leukemia cells.

Nature communications

Larrue C,Guiraud N,Mouchel PL,Dubois M,Farge T,Gotanègre M,Bosc C,Saland E,Nicolau-Travers ML,Sabatier M,Serhan N,Sahal A,Boet E,Mouche S,Heydt Q,Aroua N,Stuani L,Kaoma T,Angenendt L,Mikesch JH,Schliemann C,Vergez F,Tamburini J,Récher C,Sarry JE

44-698G was used in Western Blotting to uncover that calcitonin receptor-like receptor (CALCRL) is expressed in relapse-initiating cells, and that the overexpression of calcitonin receptor-like receptor and/or of its ligand adrenomedullin (ADM), and not CGRP, correlates to adverse outcome in acute myeloid leukemia.

Mon Jan 18 00:00:00 UTC 2021

A Metabolomics Investigation of the Metabolic Changes of Raji B Lymphoma Cells Undergoing Apoptosis Induced by Zinc Ions.

Metabolites

Yoon N,Lee H,Lee G,Kim EH,Kim SH,Lee JO,Song Y,Park J,Kim SD,Kim Y,Jung BH

44-698G was used in Western Blot to demonstrate that choline metabolism plays an important role in Zn-induced Raji cell apoptosis.

Thu Oct 07 00:00:00 UTC 2021

Long Non-coding RNA MIAT Knockdown Prevents the Formation of Intracranial Aneurysm by Downregulating ENC1 via MYC.

Frontiers in physiology

Li X,Zhao H,Liu J,Tong J

Published figure using PARP1 (cleaved Asp214, Asp215) polyclonal antibody (Product # 44-698G) in Western Blot

Wed Feb 10 00:00:00 UTC 2021

UHPLC-Q/Orbitrap/MS/MS fingerprinting and antitumoral effects of Prosopis strombulifera (LAM.) BENTH. queous extract on allograft colorectal and melanoma cancer models.

Heliyon

Persia FA,Troncoso ME,Rinaldini E,Simirgiotis M,Tapia A,Bórquez J,Mackern-Oberti JP,Hapon MB,Gamarra-Luques C

44-698G was used in Western Blotting to conclude that PsAE is composed by phenolic compounds which demonstrate cytotoxic and antitumoral properties when is orally administrated.

Sat Feb 01 00:00:00 UTC 2020

Psoriasin overexpression confers drug resistance to cisplatin by activating ERK in gastric cancer.

International journal of oncology

Li L,Cui Y,Ye L,Zhao Z,Jiang WG,Ji J

44-698G was used in Flow cytometry/Cell sorting to demonstrate the broad cellular effects of SARS-CoV-2 infection beyond adaptive immune cells and provides an entry point toward developing biomarkers and targeted treatments of patients with COVID-19.

Sat Sep 01 00:00:00 UTC 2018

Bacterial Carriers for Glioblastoma Therapy.

Molecular therapy oncolytics

Mehta N,Lyon JG,Patil K,Mokarram N,Kim C,Bellamkonda RV

44698G was used in immunohistochemistry - paraffin section to explore the use of bacterial carriers in a rat model of glioblastoma

Fri Mar 17 00:00:00 UTC 2017

Bacterial Carriers for Glioblastoma Therapy.

Molecular therapy oncolytics

Mehta N,Lyon JG,Patil K,Mokarram N,Kim C,Bellamkonda RV

44698G was used in immunohistochemistry - paraffin section to explore the use of bacterial carriers in a rat model of glioblastoma

Fri Mar 17 00:00:00 UTC 2017

The addition of abemaciclib to sunitinib induces regression of renal cell carcinoma xenograft tumors.

Oncotarget

Small J,Washburn E,Millington K,Zhu J,Holder SL

Published figure using PARP (Cleaved Asp214, Asp215) polyclonal antibody (Product # 44-698G) in Western Blot

Fri Nov 10 00:00:00 UTC 2017

Disordered IL-33/ST2 activation in decidualizing stromal cells prolongs uterine receptivity in women with recurrent pregnancy loss.

PloS one

Salker MS,Nautiyal J,Steel JH,Webster Z,Sućurović S,Nicou M,Singh Y,Lucas ES,Murakami K,Chan YW,James S,Abdallah Y,Christian M,Croy BA,Mulac-Jericevic B,Quenby S,Brosens JJ

Tue Jul 02 00:00:00 UTC 2013

Calpain 3 is important for muscle regeneration: evidence from patients with limb girdle muscular dystrophies.

BMC musculoskeletal disorders

Hauerslev S,Sveen ML,Duno M,Angelini C,Vissing J,Krag TO

Fri Mar 23 00:00:00 UTC 2012

A gatekeeper residue for NEDD8-activating enzyme inhibition by MLN4924.

Cell reports

Toth JI,Yang L,Dahl R,Petroski MD

44-698G was used in western blot to elucidate resistance to MLN4924.

Thu Apr 19 00:00:00 UTC 2012

Expression of a naturally occurring angiotensin AT(1) receptor cleavage fragment elicits caspase-activation and apoptosis.

American journal of physiology. Cell physiology

Cook JL,Singh A,DeHaro D,Alam J,Re RN

44-698G was used in immunocytochemistry to identify the cleavage sites of angiotensin type-1 receptor.

Tue Nov 01 00:00:00 UTC 2011

BRCA1-IRIS overexpression abrogates UV-induced p38MAPK/p53 and promotes proliferation of damaged cells.

Oncogene

Chock K,Allison JM,Elshamy WM

44-698G was used in western blot to study the regulation of the p38MAPK-p53-WIP1 pathway by BRCA1-IRIS in stressed and cancer cells

Thu Sep 23 00:00:00 UTC 2010

The Bax carboxy-terminal hydrophobic helix does not determine organelle-specific targeting but is essential for maintaining Bax in an inactive state and for stable mitochondrial membrane insertion.

Apoptosis : an international journal on programmed cell death

Brock SE,Li C,Wattenberg BW

44-698G was used in western blot to investigate the function of the hydrophobic carboxy-terminal tail of Bax

Fri Jan 01 00:00:00 UTC 2010

Skin cells and tissue are capable of using L-ergothioneine as an integral component of their antioxidant defense system.

Free radical biology & medicine

Markova NG,Karaman-Jurukovska N,Dong KK,Damaghi N,Smiles KA,Yarosh DB

Wed Apr 15 00:00:00 UTC 2009

Genistein inhibits radiation-induced activation of NF-kappaB in prostate cancer cells promoting apoptosis and G2/M cell cycle arrest.

BMC cancer

Raffoul JJ,Wang Y,Kucuk O,Forman JD,Sarkar FH,Hillman GG

Wed Apr 26 00:00:00 UTC 2006

Genistein inhibits radiation-induced activation of NF-kappaB in prostate cancer cells promoting apoptosis and G2/M cell cycle arrest.

BMC cancer

Raffoul JJ,Wang Y,Kucuk O,Forman JD,Sarkar FH,Hillman GG

Wed Apr 26 00:00:00 UTC 2006

CHIR-258: a potent inhibitor of FLT3 kinase in experimental tumor xenograft models of human acute myelogenous leukemia.

Clinical cancer research : an official journal of the American Association for Cancer Research

Lopes de Menezes DE,Peng J,Garrett EN,Louie SG,Lee SH,Wiesmann M,Tang Y,Shephard L,Goldbeck C,Oei Y,Ye H,Aukerman SL,Heise C

Fri Jul 15 00:00:00 UTC 2005

Induction of apoptosis in human lung cancer cells by curcumin.

Cancer letters

Radhakrishna Pillai G,Srivastava AS,Hassanein TI,Chauhan DP,Carrier E

Fri May 28 00:00:00 UTC 2004

Development of immunomodulatory six base-length non-CpG motif oligonucleotides for cancer vaccination.

Vaccine

Filion MC,Filion B,Roy J,Ménard S,Reader S,Phillips NC

Wed Jun 23 00:00:00 UTC 2004

Development of immunomodulatory six base-length non-CpG motif oligonucleotides for cancer vaccination.

Vaccine

Filion MC,Filion B,Roy J,Ménard S,Reader S,Phillips NC

Wed Jun 23 00:00:00 UTC 2004

RNA interference is a functional pathway with therapeutic potential in human myeloid leukemia cell lines.

Cancer gene therapy

Cioca DP,Aoki Y,Kiyosawa K

Sat Feb 01 00:00:00 UTC 2003

Induction of apoptosis and CD10/neutral endopeptidase expression by jaspamide in HL-60 line cells.

Cellular and molecular life sciences : CMLS

Cioca DP,Kitano K

Thu Aug 01 00:00:00 UTC 2002

Peroxisome proliferator-activated receptor gamma ligand troglitazone induces cell cycle arrest and apoptosis of hepatocellular carcinoma cell lines.

Cancer

Yoshizawa K,Cioca DP,Kawa S,Tanaka E,Kiyosawa K

Fri Nov 15 00:00:00 UTC 2002

Proliferation marker pKi-67 affects the cell cycle in a self-regulated manner.

Journal of cellular biochemistry

Schmidt MH,Broll R,Bruch HP,Duchrow M

Thu Apr 01 00:00:00 UTC 2004

Depletion of alveolar macrophages exerts protective effects in pulmonary tuberculosis in mice.

Journal of immunology (Baltimore, Md. : 1950)

Leemans JC,Juffermans NP,Florquin S,van Rooijen N,Vervoordeldonk MJ,Verbon A,van Deventer SJ,van der Poll T

Sun Apr 01 00:00:00 UTC 2001

生物信息学

蛋白别名: ADP-ribosyltransferase (NAD+; ADP-ribosyltransferase (NAD+) poly (ADP-ribose) polymerase); ADP-ribosyltransferase (NAD+, poly (ADP-ribose) polymerase) 1; ADP-ribosyltransferase (NAD+; poly (ADP-ribose) polymerase); ADP-ribosyltransferase (NAD+; poly (ADP-ribose) polymerase) 1; ADP-ribosyltransferase 1; ADP-ribosyltransferase diphtheria toxin-like 1; ADP-ribosyltransferase NAD(+); ADPRT 1; ARTD1; DNA ADP-ribosyltransferase PARP1; msPARP; NAD(+) ADP-ribosyltransferase 1; PARP-1; poly (ADP-ribose) polymerase 1; poly (ADP-ribose) polymerase family, member 1; poly (ADP-ribose) polymerase); Poly [ADP-ribose] polymerase 1; poly(ADP-ribose) polymerase; poly(ADP-ribose) polymerase PARP-1; poly(ADP-ribose) synthetase; poly(ADP-ribosyl)transferase; Poly[ADP-ribose] synthase 1; poly[ADP-ribose] synthetase 1; Protein poly-ADP-ribosyltransferase PARP1; unnamed protein product

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基因别名: 5830444G22Rik; Adprp; ADPRT; ADPRT 1; ADPRT1; AI893648; ARTD1; C80510; pADPRT-1; PARP; PARP-1; PARP1; PPOL; sPARP-1

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UniProt ID: (Bovine) P18493, (Human) P09874, (Rat) P27008

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Entrez Gene ID: (Bovine) 286764, (Human) 142, (Rat) 25591, (Mouse) 11545

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功能