Invitrogen

PAX3 Polyclonal Antibody

PAX3 Antibody in Immunocytochemistry (ICC/IF)

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PAX3 Antibody (PA1-107) in ICC/IF

Immunofluorescent analysis of Pax3 (red) in human neural stem cells derived from PD-3 iPSCs using Gibco® PSC Neural Induction Medium (Product # A1647801). The cells were fixed and permeabilized using Image-IT® Fixation/Permeabilization kit (Product # R37602), and blocked with blocking buffer included the kit for one hour at room temperature. Cells were stained with a Pax3 polyclonal antibody (Product # PA1-107) at a dilution of 1:200 in blocking buffer for 3 hours at room temperature, and then incubated with an AlexaFluor 594- conjugated d... View More

PAX3 Antibody in Immunohistochemistry (Frozen) (IHC (F))

PAX3 Antibody in Immunoprecipitation (IP)

PAX3 Antibody in Immunohistochemistry (IHC)

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产品信息

PA1-107

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:1,000

免疫组化 (IHC)

查看2篇已发表文章 2篇已发表文章

免疫组化（冰冻） (IHC (F))

1:100-1:1,000

免疫细胞化学 (ICC/IF)

1:100-1:1,000

查看1篇已发表文章 1篇已发表文章

免疫沉淀 (IP)

2 µg

产品规格

种属反应

Human, Mouse

已发表种属

Human, Mouse

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Amino acids 301-442 of human PAX3

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Protein G

保存液

PBS with 1mg/mL BSA, 30% glycerol

内含物

0.05% sodium azide

保存条件

-20°C

运输条件

Ambient (domestic); Wet ice (international)

AB_2539881

产品详细信息

PA1-107 has successfully been used in Western blot for detecting overexpressed and recombinant human Pax3 protein, but may not be suitable for detecting endogenous Pax3.

By immunofluorescence, PA1-107 has been used to stain frozen embryonic mouse brain tissue.

PA1-107 has been validated for IP using overexpressed Pax3 protein.

靶标信息

Pax 3 (Paired Box 3) is a transcription factor that is involved in development of the peripheral nervous system, melanocytes, some vascular smooth muscle, and a number of other derivatives. It regulates neurogenesis in pre-migratory neural crest cells from the dorsal neural tube, and in myogenic progenitors in the presomitic mesoderm and the hypaxial somites. Alternative splicing results in isoforms having different C-termini.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

[Screening of Mothers in a COVID-19 Low-Prevalence Region: Determination of SARS-CoV-2 Antibodies in 401 Mothers from Rostock by ELISA and Confirmation by Immunofluorescence].

Deutsche medizinische Wochenschrift (1946)

Reisinger EC,von Possel R,Warnke P,Geerdes-Fenge HF,Hemmer CJ,Pfefferle S,Löbermann M,Littmann M,Emmerich P

PA1-107 was used in Immunocytochemistry to screen 401 mothers of children between 1 and 10 years in the city of Rostock (State of Mecklenburg-Westpomerania, northeast of Germany), for the presence of RNA of SARS-CoV-2 in throat swabs, and of antibodies against SARS-CoV-2 in serum.

Sat Aug 01 00:00:00 UTC 2020

Netrin1/DCC signaling promotes neuronal migration in the dorsal spinal cord.

Neural development

Junge HJ,Yung AR,Goodrich LV,Chen Z

PA1107 was used in immunohistochemistry to test if Netrin1 ligand and ROBO3 coreceptor are important in the migration of the dorsal spinal cord progenitors and interneurons

Wed Oct 26 00:00:00 UTC 2016

NOVA regulates Dcc alternative splicing during neuronal migration and axon guidance in the spinal cord.

eLife

Leggere JC,Saito Y,Darnell RB,Tessier-Lavigne M,Junge HJ,Chen Z

PA1-107 was used in immunohistochemistry to analyze regulation of Dcc alternative splicing during neuronal migration and axon guidance in the spinal cord by NOVA

Wed May 25 00:00:00 UTC 2016

生物信息学

蛋白别名: HuP2; MGC120381; MGC120382; MGC120383; MGC120384; MGC134778; paired box gene 3; paired box homeotic gene 3; Paired box protein Pax-3; paired domain gene 3; paired domain gene HuP2; PAX3/FKHR fusion

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基因别名: CDHS; HUP2; Pax-3; PAX3; Sp; splotch; WS1; WS3

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UniProt ID: (Human) P23760, (Mouse) P24610

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Entrez Gene ID: (Human) 5077, (Mouse) 18505

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功能

transcription factor activity, sequence-specific DNA binding protein binding sequence-specific DNA binding HMG box domain binding DNA binding chromatin binding transcription factor activity, RNA polymerase II distal enhancer sequence-specific binding

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