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PAX8 Antibody (PA1-112) in ICC/IF
Immunofluorescent analysis of PAX8 (green) in 293T (left panel) and COS7 cells (right panel). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature. Cells were blocked with 1% Blocker BSA (Product # 37525) for 15 minutes at room temperature. Cells were probed with a PAX8 polyclonal antibody (Product # PA1-112) at a dilution of 1:400 for at least 1 hour at room temperature, washed with PBS, and incubated with a DyLight 488-conjugated goat anti-rabbit IgG secondary antibody (Product # 35552)... View More
产品信息
PA1-112
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Non-human primate
已发表种属
Human
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Purified internal fragment of human recombinant PAX8 expressed in E. coli
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Protein G
保存液
PBS with 1mg/mL BSA, 30% glycerol
内含物
0.05% sodium azide
保存条件
-20°C
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_2539884
产品详细信息
Western blot analysis of PA1-112 detects a prominent ~48 kDa protein in human adenocarcinoma SKOV-3 and SV40 transformed African green monkey (Cercopithecus aethiops) kidney cells. In SKOV-3 cells, 2 additional unknown bands at (~20 kD) and at ~150 kDa are also detected. Specificity of the antibody was confirmed in HeLa and 293T cells overexpressing full lenght PAX8. The 48 kDa band is enriched in immunoprecipitations from Cos7 lysate, however additional unknown high molecular weight bands are also enriched and detected by PA1-112.
靶标信息
This gene encodes a member of the paired box family of transcription factors. Members of this gene family typically encode proteins that contain a paired box domain, an octapeptide, and a paired-type homeodomain. This nuclear protein is involved in thyroid follicular cell development and expression of thyroid-specific genes. Mutations in this gene have been associated with thyroid dysgenesis, thyroid follicular carcinomas and atypical follicular thyroid adenomas. Alternatively spliced transcript variants encoding different isoforms have been described.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: HGNC:8622; Paired box protein Pax-8; paired domain gene 8
基因别名: PAX8
UniProt ID: (Human) Q06710
Entrez Gene ID: (Human) 7849
RNA polymerase II core promoter proximal region sequence-specific DNA binding
RNA polymerase II core promoter sequence-specific DNA binding
transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding
DNA binding
transcription factor activity, sequence-specific DNA binding
thyroid-stimulating hormone receptor activity
protein binding
transcription regulatory region DNA binding
urogenital system development
branching involved in ureteric bud morphogenesis
kidney development
mesonephros development
mesenchymal to epithelial transition involved in metanephros morphogenesis
transcription, DNA-templated
transcription from RNA polymerase II promoter
sulfur compound metabolic process
central nervous system development
anatomical structure morphogenesis
thyroid gland development
thyroid-stimulating hormone signaling pathway
pronephric field specification
inner ear morphogenesis
regulation of apoptotic process
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
pronephros development
cellular response to gonadotropin stimulus
otic vesicle development
positive regulation of mesenchymal to epithelial transition involved in metanephros morphogenesis
metanephric epithelium development
metanephric distal convoluted tubule development
metanephric comma-shaped body morphogenesis
metanephric S-shaped body morphogenesis
metanephric nephron tubule formation
negative regulation of mesenchymal cell apoptotic process involved in metanephric nephron morphogenesis
regulation of metanephric nephron tubule epithelial cell differentiation
positive regulation of branching involved in ureteric bud morphogenesis
negative regulation of mesenchymal cell apoptotic process involved in metanephros development
negative regulation of apoptotic process involved in metanephric collecting duct development
negative regulation of apoptotic process involved in metanephric nephron tubule development
positive regulation of metanephric DCT cell differentiation
positive regulation of thyroid hormone generation
regulation of thyroid-stimulating hormone secretion
