Invitrogen

PF4 Polyclonal Antibody, PeproTech®

10 篇参考文献

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抗体检测数据 (3)

PF4 Antibody in Western Blot (WB)

PF4 Antibody in Western Blot (WB)

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PF4 Antibody (500-P05-1MG) in WB

Western Blot: To detect Human PF-4 (CXCL4) by Western Blot analysis PF4 Polyclonal Antibody (Product # 500-P05-1MG) can be used at a concentration of 0.1-0.2 µg/mL. When used in conjunction with compatible secondary reagents the detection limit for Recombinant Human PF-4 (CXCL4) is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

PF4 Antibody in Western Blot (WB)

PF4 Antibody in Western Blot (WB)

PF4 Antibody in ELISA (ELISA)

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PF4 Antibody in Western Blot (WB) — Western Blot: To detect Human PF-4 (CXCL4) by Western Blot analysis PF4 Polyclonal Antibody (Product # 500-P05-1MG) can be used at a concentration of 0.1-0.2 µg/mL. When used in conjunction with compatible secondary reagents the detection limit for Recombinant Human PF-4 (CXCL4) is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

PF4 Antibody in Western Blot (WB) — Western Blot: To detect Human PF-4 (CXCL4) by Western Blot analysis PF4 Polyclonal Antibody (Product # 500-P05-1MG) can be used at a concentration of 0.1-0.2 µg/mL. When used in conjunction with compatible secondary reagents the detection limit for Recombinant Human PF-4 (CXCL4) is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

PF4 Antibody in ELISA (ELISA) — Sandwich ELISA: To detect Human PF-4 (CXCL4) by sandwich ELISA (using 100 µL/well) a concentration of 0.5-2.0 µg/mL of PF4 Polyclonal Antibody (Product # 500-P05-1MG) is required. This antigen affinity purified antibody, in conjunction with PeproTech PF4 Polyclonal Antibody, Biotin (Product # 500-P05BT-1MG) as a detection antibody, allows the detection of at least 2000-4000 pg/mL of Recombinant Human PF-4 (CXCL4).

产品信息

500-P05-1MG

应用

建议稀释比

已发表文章

免疫印迹 (WB)

0.1-0.2 µg/mL

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流式细胞分析 (Flow)

-

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酶联免疫吸附实验 (ELISA)

0.5-2.0 µg/mL

-

免疫沉淀 (IP)

-

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中和实验 (Neu)

-

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其他PubMed (Misc)

-

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免疫染色 (IS)

-

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产品规格

种属反应

Human

已发表种属

Human, Mouse

宿主/亚型

Rabbit

分类

Polyclonal

类型

Antibody

抗原

E.coli-derived Recombinant Human PF-4 (CXCL4)

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Lyophilized

浓度

0.1-1.0 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS

内含物

no preservative

保存条件

-20°C

运输条件

Ambient

AB_2929160

产品详细信息

AA Sequence of recombinant protein: EAEEDGDLQC LCVKTTSQVR PRHITSLEVI KAGPHCPTAQ LIATLKNGRK ICLDLQAPLY KKIIKKLLES.

Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human PF-4 (CXCL4). Anti-Human PF-4 (CXCL4)-specific antibody was purified by affinity chromatography employing an immobilized Human PF-4 matrix.

Sandwich ELISA: To detect Human PF-4 (CXCL4) by sandwich ELISA (using 100 µL/well) a concentration of 0.5-2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Human PF-4 (CXCL4) (500-P05BT) as a detection antibody, allows the detection of at least 2000-4000 pg/mL of Recombinant Human PF-4 (CXCL4).

Western Blot: To detect Human PF-4 (CXCL4) by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. When used in conjunction with compatible secondary reagents the detection limit for Recombinant Human PF-4 (CXCL4) is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

靶标信息

Platelet factor-4 is a 70-amino acid protein that is released from the alpha-granules of activated platelets and binds with high affinity to heparin. Its major physiologic role appears to be neutralization of heparin-like molecules on the endothelial surface of blood vessels, thereby inhibiting local antithrombin III activity and promoting coagulation. As a strong chemoattractant for neutrophils and fibroblasts, PF4 probably has a role in inflammation and wound repair (Eisman et al., 1990).

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Recognition of PF4-VWF complexes by heparin-induced thrombocytopenia antibodies contributes to thrombus propagation.

Blood

Johnston I,Sarkar A,Hayes V,Koma GT,Arepally GM,Chen J,Chung DW,López JA,Cines DB,Rauova L,Poncz M

Thu Apr 09 00:00:00 UTC 2020

Platelet factor 4 mediates vascular smooth muscle cell injury responses.

Blood

Shi G,Field DJ,Long X,Mickelsen D,Ko KA,Ture S,Korshunov VA,Miano JM,Morrell CN

Thu May 23 00:00:00 UTC 2013

Identification of the platelet-derived chemokine CXCL4/PF-4 as a broad-spectrum HIV-1 inhibitor.

Proceedings of the National Academy of Sciences of the United States of America

Auerbach DJ,Lin Y,Miao H,Cimbro R,Difiore MJ,Gianolini ME,Furci L,Biswas P,Fauci AS,Lusso P

Tue Jun 12 00:00:00 UTC 2012

Identification of the platelet-derived chemokine CXCL4/PF-4 as a broad-spectrum HIV-1 inhibitor.

Proceedings of the National Academy of Sciences of the United States of America

Auerbach DJ,Lin Y,Miao H,Cimbro R,Difiore MJ,Gianolini ME,Furci L,Biswas P,Fauci AS,Lusso P

Tue Jun 12 00:00:00 UTC 2012

Serglycin proteoglycan deletion induces defects in platelet aggregation and thrombus formation in mice.

Blood

Woulfe DS,Lilliendahl JK,August S,Rauova L,Kowalska MA,Abrink M,Pejler G,White JG,Schick BP

Tue Apr 01 00:00:00 UTC 2008

Platelet factor 4 is a negative autocrine in vivo regulator of megakaryopoiesis: clinical and therapeutic implications.

Blood

Lambert MP,Rauova L,Bailey M,Sola-Visner MC,Kowalska MA,Poncz M

Wed Aug 15 00:00:00 UTC 2007

Role of platelet surface PF4 antigenic complexes in heparin-induced thrombocytopenia pathogenesis: diagnostic and therapeutic implications.

Blood

Rauova L,Zhai L,Kowalska MA,Arepally GM,Cines DB,Poncz M

Wed Mar 15 00:00:00 UTC 2006

Ultralarge complexes of PF4 and heparin are central to the pathogenesis of heparin-induced thrombocytopenia.

Blood

Rauova L,Poncz M,McKenzie SE,Reilly MP,Arepally G,Weisel JW,Nagaswami C,Cines DB,Sachais BS

Sat Jan 01 00:00:00 UTC 2005

Transgenic mice studies demonstrate a role for platelet factor 4 in thrombosis: dissociation between anticoagulant and antithrombotic effect of heparin.

Blood

Eslin DE,Zhang C,Samuels KJ,Rauova L,Zhai L,Niewiarowski S,Cines DB,Poncz M,Kowalska MA

Mon Nov 15 00:00:00 UTC 2004

Platelet factor 4 enhances the binding of oxidized low-density lipoprotein to vascular wall cells.

The Journal of biological chemistry

Nassar T,Sachais BS,Akkawi S,Kowalska MA,Bdeir K,Leitersdorf E,Hiss E,Ziporen L,Aviram M,Cines D,Poncz M,Higazi AA

Fri Feb 21 00:00:00 UTC 2003

生物信息学

蛋白别名: C-X-C motif chemokine 4; chemokine (C-X-C motif) ligand 4; Iroplact; Oncostatin-A; PF-4; Platelet factor 4

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基因别名: CXCL4; PF-4; PF4; SCYB4

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UniProt ID: (Human) P02776

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Entrez Gene ID: (Human) 5196

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chemokine activity heparin binding CXCR3 chemokine receptor binding chemokine

platelet degranulation positive regulation of leukocyte chemotaxis inflammatory response immune response G-protein coupled receptor signaling pathway positive regulation of gene expression positive regulation of macrophage derived foam cell differentiation negative regulation of angiogenesis cytokine-mediated signaling pathway platelet activation leukocyte chemotaxis positive regulation of cAMP metabolic process response to lipopolysaccharide positive regulation of tumor necrosis factor production regulation of cell proliferation positive regulation of cAMP-mediated signaling negative regulation of MHC class II biosynthetic process positive regulation of macrophage differentiation negative regulation of megakaryocyte differentiation negative regulation of cytolysis positive regulation of transcription from RNA polymerase II promoter chemokine-mediated signaling pathway negative regulation of extrinsic apoptotic signaling pathway in absence of ligand