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产品信息
PA1-073
应用
建议稀释比
已发表文章
产品规格
种属反应
Mouse,
Rat
已发表种属
Mouse,
Non-human primate
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Synthetic peptide corresponding to residues M(1) F A D L D Y D I E E D K L G I P T(18) of mouse PICK1.
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Antigen affinity chromatography
保存液
PBS with 1mg/mL BSA
内含物
0.05% sodium azide
保存条件
-20° C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_2252424
产品详细信息
PA1-073 detects protein interacting with protein kinase C (PICK1) from mouse cells.
PA1-073 has been successfully used in Western blot, immunocytochemistry, and immunoprecipitation procedures. By Western blot, this antibody detects an ~52 kDa protein representing PICK1 from AtT20 cell extract. Immunocytochemical staining of CV1 cells transfected with PICK1 results in a diffuse staining pattern when expressed alone and coclustered when coexpressed with mGluR7.
The PA1-073 immunogen is a synthetic peptide corresponding to residues M(1) F A D L D Y D I E E D K L G I P T(18) of mouse PICK1. The PA1-073 immunizing peptide (Cat. # PEP-133) is available for use in neutralization and control experiments.
靶标信息
PSD-95/DLG/ZO-1 (PDZ) domain-containing proteins play a central role in synaptic membrane protein localization. The protein interacting with protein kinase C (PICK1) is a synaptic PDZ domain protein that also contains a coiled-coil and acidic domain. Studies indicate that PICK1 functions as a targeting and transport protein and has been shown to interact with protein kinase C alpha (PKCalpha), AMPA-type glutamate receptors, and several other membrane receptors via its PDZ domain. The interaction of PICK1 with PKCalpha is highly dependent on the activation of the kinase. It appears that PICK1 directs the activated form of PKCalpha to membrane anchored GluR2. Once phosphorylated by PKCalpha, GluR2 is released from the synaptic anchor proteins. Once released, GluR2 is transported from the synaptic membrane in a PICK1-dependent manner.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: MGC15204; OTTHUMP00000028509; PRKCA-binding protein; Protein interacting with C kinase 1; protein kinase C, alpha binding protein; Protein kinase C-alpha-binding protein; protein that interacts with C kinase 1
基因别名: Pick1; Prkcabp
UniProt ID: (Rat) Q9EP80
Entrez Gene ID: (Mouse) 18693, (Rat) 84591
G-protein coupled receptor binding
actin binding
protein kinase C binding
receptor binding
protein binding
protein C-terminus binding
ATPase activity
enzyme binding
protein domain specific binding
identical protein binding
metal ion binding
actin filament binding
Arp2/3 complex binding
kinase activity
receptor tyrosine kinase binding
G-protein coupled glutamate receptor binding
ephrin receptor binding
GTPase binding
positive regulation of receptor internalization
protein phosphorylation
protein targeting
protein kinase C-activating G-protein coupled receptor signaling pathway
multicellular organism development
negative regulation of gene expression
monoamine transport
glial cell development
regulation of Arp2/3 complex-mediated actin nucleation
negative regulation of Arp2/3 complex-mediated actin nucleation
intracellular signal transduction
cellular response to decreased oxygen levels
cellular response to glucose starvation
receptor clustering
regulation of synaptic plasticity
regulation of synapse structure or activity
protein homooligomerization
long term synaptic depression
negative regulation of cell death
dendritic spine organization
dendritic spine maintenance
dopamine transport
phosphorylation
