Invitrogen

PKP3 Monoclonal Antibody (23E3/4)

图: 1 / 13

PKP3 Antibody (35-7600) in WB

Western blot analysis was performed on whole cell extracts (30 µg lysate) of A-431 (Lane 1), A549 (Lane 2), HEK-293 (Lane 3), SK-OV-3 (Lane 4) and COLO 205 (Lane 5). The blots were probed with Mouse Anti-PKP3 Monoclonal Antibody (Product # 35-7600, 2 µg/mL) and detected by chemiluminescence using Goat anti-Mouse IgG (H+L) Superclonal Secondary Antibody, HRP conjugate (Product # A28177, 0.25 µg/mL, 1:4000 dilution). A 87 kDa band corresponding to PKP3 was observed across the cell lines tested. Known quantity of protein samples were electrop... View More

PKP3 Antibody in Western Blot, Immunocytochemistry (WB, ICC/IF)

PKP3 Antibody in Immunocytochemistry (ICC/IF)

PKP3 Antibody in Immunohistochemistry (IHC)

PKP3 Antibody in Western Blot, ChIP Assay (WB, ChIP)

Product Image Gallery

PKP3 Antibody — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. HeLa cells were transfected with Plakophilin-3 siRNA and loss of signal was observed in Western Blot using Anti-Plakophilin-3 Antibody (Product # 35-7600). {KD}

产品信息

35-7600

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1-3 µg/mL

查看6篇已发表文章 6篇已发表文章

免疫组化 (IHC)

Assay-dependent

查看3篇已发表文章 3篇已发表文章

免疫细胞化学 (ICC/IF)

查看3篇已发表文章 3篇已发表文章

酶联免疫吸附实验 (ELISA)

Assay-dependent

免疫沉淀 (IP)

Assay-dependent

染色质免疫沉淀实验 (ChIP)

查看1篇已发表文章 1篇已发表文章

其他PubMed (Misc)

查看2篇已发表文章 2篇已发表文章

产品规格

种属反应

Human

已发表种属

Human, Mouse

宿主/亚型

Mouse / IgG2b

分类

Monoclonal

类型

Antibody

克隆号

23E3/4

抗原

Synthetic peptide from Armadillo domain of human Plakophilin-3.

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Protein A

保存液

PBS, pH 7.4

内含物

0.1% sodium azide

保存条件

-20°C

运输条件

Ambient (domestic); Wet ice (international)

AB_2533221

靶标信息

Plakophilin-3 is an Armadillo-like protein present in nuclei and desmosomes of epithelial cells. The expression pattern of this protein seems to be largely restricted to epithelial cell types. Plakophilin-3 can be detected along cell borders in a punctuate staining pattern typical for desmosomal proteins. In addition to the desmosomal immunolocalization, immunostaining was observed as bright nuclear speckles. Thus, like plakophilin-1 and-2, plakophilin-3 displays a dual intracellular localization in the desmosomal plaque and in the cell nucleus, and therefore is probably involved in signal transduction pathways between the plasma membrane and the nucleus. The human protein has a predicted molecular mass of ~87 kDa.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Disruption of desmosome function leads to increased centrosome clustering in 14-3-3γ-knockout cells with supernumerary centrosomes.

FEBS letters

Tilwani S,Gandhi K,Narayan S,Ainavarapu SRK,Dalal SN

Published figure using PKP3 monoclonal antibody (Product # 35-7600) in Immunocytochemistry

Mon Nov 01 00:00:00 UTC 2021

Disruption of desmosome function leads to increased centrosome clustering in 14-3-3γ-knockout cells with supernumerary centrosomes.

FEBS letters

Tilwani S,Gandhi K,Narayan S,Ainavarapu SRK,Dalal SN

Published figure using PKP3 monoclonal antibody (Product # 35-7600) in Immunocytochemistry

Mon Nov 01 00:00:00 UTC 2021

Proteomic characterization of GSK3β knockout shows altered cell adhesion and metabolic pathway utilisation in colorectal cancer cells.

PloS one

Bowler-Barnett E,Martinez-Garcia FD,Sherwood M,Aleidan A,John S,Weston S,Wang Y,Divecha N,Skipp P,Ewing RM

35-7600 was used in Western Blot to identify several of the diverse pathways and processes associated with GSK3β function.

Wed Dec 29 00:00:00 UTC 2021

Proteomic characterization of GSK3β knockout shows altered cell adhesion and metabolic pathway utilisation in colorectal cancer cells.

PloS one

Bowler-Barnett E,Martinez-Garcia FD,Sherwood M,Aleidan A,John S,Weston S,Wang Y,Divecha N,Skipp P,Ewing RM

35-7600 was used in Western Blot to identify several of the diverse pathways and processes associated with GSK3β function.

Wed Dec 29 00:00:00 UTC 2021

Increased Cholesterol Biosynthesis Is a Key Characteristic of Breast Cancer Stem Cells Influencing Patient Outcome.

Cell reports

Ehmsen S,Pedersen MH,Wang G,Terp MG,Arslanagic A,Hood BL,Conrads TP,Leth-Larsen R,Ditzel HJ

35-7600 was used in Immunohistochemistry to identify the cholesterol biosynthesis pathway as central for CSC propagation and a potential therapeutic target, as well as providing a mechanistic explanation for the therapeutic benefit of statins in breast cancer.

Tue Jun 25 00:00:00 UTC 2019

MMP7 is required to mediate cell invasion and tumor formation upon Plakophilin3 loss.

PloS one

Basu S,Thorat R,Dalal SN

35-7600 was used in western blot to investigate the role of MMP7 in cell invasion and tumor formation

Wed Jan 13 00:00:00 UTC 2016

Plakophilin3 loss leads to an increase in PRL3 levels promoting K8 dephosphorylation, which is required for transformation and metastasis.

PloS one

Khapare N,Kundu ST,Sehgal L,Sawant M,Priya R,Gosavi P,Gupta N,Alam H,Karkhanis M,Naik N,Vaidya MM,Dalal SN

35-7600 was used in western blot to investigate the effects of K8 filament stabilization

Thu Nov 08 00:00:00 UTC 2012

Plakophilin3 loss leads to an increase in PRL3 levels promoting K8 dephosphorylation, which is required for transformation and metastasis.

PloS one

Khapare N,Kundu ST,Sehgal L,Sawant M,Priya R,Gosavi P,Gupta N,Alam H,Karkhanis M,Naik N,Vaidya MM,Dalal SN

35-7600 was used in western blot to investigate the effects of K8 filament stabilization

Thu Nov 08 00:00:00 UTC 2012

Unimpaired skin carcinogenesis in Desmoglein 3 knockout mice.

PloS one

Baron S,Hoang A,Vogel H,Attardi LD

35-7600 was used in immunocytochemistry to explore the role of desmosomal cadherins in carcinogenesis.

Thu May 30 00:00:00 UTC 2013

E-cadherin and plakoglobin recruit plakophilin3 to the cell border to initiate desmosome assembly.

Cellular and molecular life sciences : CMLS

Gosavi P,Kundu ST,Khapare N,Sehgal L,Karkhanis MS,Dalal SN

35-7600 was used in immunocytochemistry and western blot to test if plakophilin3 is required for desmosome formation

Fri Apr 01 00:00:00 UTC 2011

The transcription factor ZEB1 (deltaEF1) represses Plakophilin 3 during human cancer progression.

FEBS letters

Aigner K,Descovich L,Mikula M,Sultan A,Dampier B,Bonné S,van Roy F,Mikulits W,Schreiber M,Brabletz T,Sommergruber W,Schweifer N,Wernitznig A,Beug H,Foisner R,Eger A

35-7600 was used in ChIP assay, immunocytochemistry, immunohistochemistry, and western blot to show that PKP3 is transcriptionally repressed by ZEB1 in metastatic cancer cells

Tue Apr 17 00:00:00 UTC 2007

The transcription factor ZEB1 (deltaEF1) represses Plakophilin 3 during human cancer progression.

FEBS letters

Aigner K,Descovich L,Mikula M,Sultan A,Dampier B,Bonné S,van Roy F,Mikulits W,Schreiber M,Brabletz T,Sommergruber W,Schweifer N,Wernitznig A,Beug H,Foisner R,Eger A

35-7600 was used in ChIP assay, immunocytochemistry, immunohistochemistry, and western blot to show that PKP3 is transcriptionally repressed by ZEB1 in metastatic cancer cells

Tue Apr 17 00:00:00 UTC 2007

The transcription factor ZEB1 (deltaEF1) represses Plakophilin 3 during human cancer progression.

FEBS letters

Aigner K,Descovich L,Mikula M,Sultan A,Dampier B,Bonné S,van Roy F,Mikulits W,Schreiber M,Brabletz T,Sommergruber W,Schweifer N,Wernitznig A,Beug H,Foisner R,Eger A

35-7600 was used in ChIP assay, immunocytochemistry, immunohistochemistry, and western blot to show that PKP3 is transcriptionally repressed by ZEB1 in metastatic cancer cells

Tue Apr 17 00:00:00 UTC 2007

The transcription factor ZEB1 (deltaEF1) represses Plakophilin 3 during human cancer progression.

FEBS letters

Aigner K,Descovich L,Mikula M,Sultan A,Dampier B,Bonné S,van Roy F,Mikulits W,Schreiber M,Brabletz T,Sommergruber W,Schweifer N,Wernitznig A,Beug H,Foisner R,Eger A

35-7600 was used in ChIP assay, immunocytochemistry, immunohistochemistry, and western blot to show that PKP3 is transcriptionally repressed by ZEB1 in metastatic cancer cells

Tue Apr 17 00:00:00 UTC 2007

Plakophilin 3 oncogene as prognostic marker and therapeutic target for lung cancer.

Cancer research

Furukawa C,Daigo Y,Ishikawa N,Kato T,Ito T,Tsuchiya E,Sone S,Nakamura Y

Mon Aug 15 00:00:00 UTC 2005

Plakophilin 3 oncogene as prognostic marker and therapeutic target for lung cancer.

Cancer research

Furukawa C,Daigo Y,Ishikawa N,Kato T,Ito T,Tsuchiya E,Sone S,Nakamura Y

Mon Aug 15 00:00:00 UTC 2005

生物信息学

蛋白别名: plakophilin 3b; Plakophilin-3

查看更多收起

基因别名: PKP3

查看更多收起

UniProt ID: (Human) Q9Y446

查看更多收起

Entrez Gene ID: (Human) 11187

查看更多收起

功能

alpha-catenin binding cell adhesion molecule binding cadherin binding involved in cell-cell adhesion intermediate filament binding protein

参与通路

desmosome assembly single organismal cell-cell adhesion establishment of protein localization to plasma membrane

PKP3 Monoclonal Antibody (23E3/4)

PKP3 Antibody (35-7600) in WB

产品信息

35-7600

免疫印迹 (WB)

免疫组化 (IHC)

免疫细胞化学 (ICC/IF)

酶联免疫吸附实验 (ELISA)

免疫沉淀 (IP)

染色质免疫沉淀实验 (ChIP)

其他PubMed (Misc)

种属反应

已发表种属

宿主/亚型

分类

类型

克隆号

抗原

偶联物

形式

浓度

纯化类型

保存液

内含物

保存条件

运输条件

RRID

靶标信息

生物信息学

性能保障

随时为您服务

Your items have has been added!

PKP3 Monoclonal Antibody (23E3/4)

PKP3 Antibody (35-7600) in WB

产品信息

35-7600

免疫印迹 (WB)

免疫组化 (IHC)

免疫细胞化学 (ICC/IF)

酶联免疫吸附实验 (ELISA)

免疫沉淀 (IP)

染色质免疫沉淀 实验 (ChIP)

其他PubMed (Misc)

种属反应

已发表种属

宿主/亚型

分类

类型

克隆号

抗原

偶联物

形式

浓度

纯化类型

保存液

内含物

保存条件

运输条件

RRID

靶标信息

生物信息学

性能保障

随时为您服务

Your items have has been added!

染色质免疫沉淀实验 (ChIP)