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PLOD1 Antibody (PA5-121155) in WB
Western blot analysis of PLOD1 in extracts of various cell lines. Samples were incubated with PLOD1 Polyclonal antibody (Product # PA5-121155) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.
产品信息
PA5-121155
产品规格
种属反应
Human,
Mouse,
Rat
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Recombinant fusion protein containing a sequence corresponding to amino acids 19-295 of human PLOD1 (NP_000293.2).
偶联物
形式
Liquid
浓度
1.26 mg/mL
纯化类型
Affinity Chromatography
保存液
PBS, pH 7.3, with 50% glycerol
内含物
0.05% ProClin 300
保存条件
-20° C, Avoid Freeze/Thaw Cycles
运输条件
Wet ice
RRID
AB_2914727
产品详细信息
Positive test controls include: HepG2, A-431, U-251MG, Mouse kidney, Mouse heart. The target is usually found in the following locations: Lumenal side, Peripheral membrane protein, Rough endoplasmic reticulum membrane.
Immunogen sequence: KGDAKPEDNL LVLTVATKET EGFRRFKRSA QFFNYKIQAL GLGEDWNVEK GTSAGGGQKV RLLKKALEKH ADKEDLVILF ADSYDVLFAS GPRELLKKFR QARSQVVFSA EELIYPDRRL ETKYPVVSDG KRFLGSGGFI GYAPNLSKLV AEWEGQDSDS DQLFYTKIFL DPEKREQINI TLDHRCRIFQ NLDGALDEVV LKFEMGHVRA RNLAYDTLPV LIHGNGPTKL QLNYLGNYIP RFWTFETGCT VCDEGLRSLK GIGDEALPTV LVGVFIE
靶标信息
Forms hydroxylysine residues in -Xaa-Lys-Gly- sequences in collagens. These hydroxylysines serve as sites of attachment for carbohydrate units and are essential for the stability of the intermolecular collagen cross-links.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: LH1; lysine hydroxylase; lysyl hydroxlase 1; Lysyl hydroxylase 1; procollagen-lysine 1, 2-oxoglutarate 5-dioxygenase 1; procollagen-lysine 2-oxoglutarate 5-dioxygenase (lysine hydroxylase Ehlers-Danlos syndrome type VI); procollagen-lysine, 2-oxoglutarate 5-dioxygenase (lysine hydroxylase, Ehlers-Danlos syndrome type VI); procollagen-lysine, 2-oxoglutarate 5-dioxygenase 1; Procollagen-lysine,2-oxoglutarate 5-dioxygenase 1
基因别名: 2410042F05Rik; AI854890; AV007929; EDS6; LH; LH1; LLH; PLOD; PLOD1
UniProt ID: (Human) Q02809, (Mouse) Q9R0E2
Entrez Gene ID: (Human) 5351, (Mouse) 18822, (Rat) 116552
ferrous iron binding
procollagen-lysine 5-dioxygenase activity
L-ascorbic acid binding
peptide binding
protein homodimerization activity
iron ion binding
oxidoreductase activity
oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen
oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, 2-oxoglutarate as one donor, and incorporation of one atom each of oxygen into both donors
metal ion binding
dioxygenase activity
protein modifying enzyme
