Invitrogen

PPBP Polyclonal Antibody

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PPBP Antibody (PA5-47070) in Neu

Neutralization of PPBP in BaF3 mouse pro‚B cell line transfected with human CXCR2. Samples were incubated in PPBP polyclonal antibody (Product # PA5-47070). Recombinant Rat CXCL7/Thymus Chemokine‚1 chemoattracts the BaF3 mouse pro‚B cell line transfected with human CXCR2 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin. Chemotaxis elicited by Recombinant Rat CXCL7/Thymus Chemokine‚1 (0.04 µg/mL) is neutralized (green line) by increasing concentrati... View More

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产品信息

PA5-47070

应用

建议稀释比

已发表文章

免疫印迹 (WB)

0.1 µg/mL

免疫组化 (IHC)

5-15 µg/mL

中和实验 (Neu)

0.8-4.0 µg/mL

产品规格

种属反应

Rat

宿主/亚型

Goat / IgG

分类

Polyclonal

类型

Antibody

抗原

E. coli-derived recombinant rat CXCL7/Thymus Chemokine-1 Ile46-Ile107

偶联物

Unconjugated Unconjugated Unconjugated

形式

Lyophilized

浓度

0.2 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS with 5% trehalose

内含物

No Preservative

保存条件

-20° C, Avoid Freeze/Thaw Cycles

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_2610675

产品详细信息

Reconstitute at 0.2 mg/mL in sterile PBS.

Endoxin level is <0.10 EU per 1 µg of the antibody by the LAL method.

靶标信息

Members of the a-chemokine subfamily of inducible, secreted, pro-inflammatory cytokines contain a similar motif, in which the first two cysteine residues are separated by a single residue (Cys-X-Cys), and are also chemotactic for neutrophils. The platelet basic protein (PBP), a member of the a-chemokine family, resides in the a-granules of platelets and is released upon their activation. Proteolytic cleavage of the amino terminus of PBP leads to the generation of several peptides, which include mature PBP, connective tissue-activating peptide III (CTAP III, also designated low affinity platelet factor IV (LA-PF4)), b-thromboglobulin (b-TG), and neutrophil-activating peptide 2 (NAP-2). PBP and its N-truncated derivatives mediate inflammation and wound healing. Specifically, NAP-2 activates chemotaxis and degranulation in neutrophils during inflammation. The gene encoding human PBP maps to chromosome 4q12-q13.

仅用于科研。不用于诊断过程。未经明确授权不得转售。