Invitrogen

PRMT5 Recombinant Rabbit Monoclonal Antibody (2H25L22)

PRMT5 Antibody in Immunocytochemistry (ICC/IF)

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PRMT5 Antibody (703704) in ICC/IF

For immunofluorescence analysis, MCF-7 cells were fixed and permeabilized using ice cold 100% Acetone for detection of endogenous PRMT5 using Anti-PRMT5 Recombinant Rabbit Monoclonal Antibody (Product # 703704, 1:100 dilution) and labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034, 1:2000). Panel a) shows representative cells that were stained for detection and localization of PRMT5 protein (green), Panel b) is stained for nuclei (blue) using ProLong™ Diamond Antifa... View More

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PRMT5 Antibody — Antibody specificity was demonstrated by siRNA mediated knockdown of the target protein. MCF7 cells were transfected with PRMT5 siRNA and a decrease in signal intensity was observed in Western blot application using Anti-PRMT5 Recombinant Rabbit Monoclonal Antibody (Product # 703704). {KD}

产品信息

703704

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:5,000

免疫细胞化学 (ICC/IF)

1:100

产品规格

种属反应

Human

宿主/亚型

Rabbit / IgG

Expression System

Expi293

分类

Recombinant Monoclonal

类型

Antibody

克隆号

2H25L22

抗原

Protein corresponding to human PRMT5 [aa1-aa291]

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Protein A

保存液

PBS, pH 7.4

内含物

0.09% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

AB_2784600

产品详细信息

This antibody is predicted to react with Monkey, Cat, Dog.

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

靶标信息

PRMT5 (protein arginine N-methyltransferase 5) is an arginine methyltransferase that can both catalyze the formation of omega-N monomethylarginine (MMA) and symmetrical dimethylarginine (sDMA), with a preference for the formation of MMA. PRMT5 specifically mediates the symmetrical dimethylation of arginine residues in the small nuclear ribonucleoproteins Sm D1 and Sm D3; such methylation is required for the assembly and biogenesis of snRNP core particles.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: 72 kDa ICln-binding protein; EC 2.1.1.125; Histone-arginine N-methyltransferase PRMT5; HMT1 hnRNP methyltransferase-like 5; Jak-binding protein 1; PRMT5; Protein arginine N-methyltransferase 5; Shk1 kinase-binding protein 1 homolog; SKB1 homolog

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基因别名: HRMT1L5; IBP72; JBP1; PRMT5; SKB1; SKB1Hs

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UniProt ID: (Human) O14744

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Entrez Gene ID: (Human) 10419

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功能