Invitrogen

PSEN2 Monoclonal Antibody (APS 26)

PSEN2 Antibody in Immunocytochemistry (ICC/IF)

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PSEN2 Antibody (MA1-754) in ICC/IF

Immunofluorescent analysis of Presenilin 2 using Anti-Presenilin 2 Monoclonal Antibody (APS 26) (Product # MA1-754) shows staining in A2058 Cells. Presenilin 2 staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing Presenilin 2 (Product # MA1-754) at a dilution of 1:20 over night at 4°C, washed with PBS and incubated with a DyLight-488 conjugated sec... View More

PSEN2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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PSEN2 Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across cell lines owing to their inherent genetic constitution. Relative expression of PSEN2 was observed in THP-1 when compared to Raji and Jurkat using Anti-PSEN2 Monoclonal Antibody (APS 26) (Product # MA1-754) in Western Blot. {RE}

产品信息

MA1-754

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:100-1:1,000

免疫组化（石蜡） (IHC (P))

1:20

免疫细胞化学 (ICC/IF)

Assay-dependent

酶联免疫吸附实验 (ELISA)

4.5 µg/mL

免疫沉淀 (IP)

Assay-dependent

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产品规格

种属反应

Human, Mouse, Non-human primate, Rat

已发表种属

Human

宿主/亚型

Mouse / IgG1

分类

Monoclonal

类型

Antibody

克隆号

APS 26

抗原

Synthetic peptide corresponding to residues C L(317) P Y D P E M E E D S Y D S F G E P(334) of human PS2.

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Protein G

保存液

PBS with 1mg/mL BSA

内含物

0.05% sodium azide

保存条件

-20° C, Avoid Freeze/Thaw Cycles

运输条件

Ambient (domestic); Wet ice (international)

AB_325093

产品详细信息

MA1-754 detects presenilin 2 protein (PS2) from rat, mouse, human, and nonhuman primate samples. No cross-reactivity is seen with presenilin 1.

MA1-754 has successfully been used in immuno-fluorescence, immunocytochemistry, Western blot and ELISA procedures. By Western blot, this antibody detects an ~20 kDa protein representing PS2 CT (C-terminal fragment) and the ~45 kDa full-length PS2 from transfected COS-7 cells. In 4% paraformaldehyde fixed RAW cells, MA1-754 showed specific Golgi and ER labeling.

The MA1-754 immunogen is a synthetic peptide corresponding to residues C L(317) P Y D P E M E E D S Y D S F G E P(334) of human PS2.

靶标信息

Alzheimer's disease (AD) patients with an inherited form of the disease carry mutations in the presenilin proteins (PSEN1 or PSEN2) or the amyloid precursor protein (APP). These disease-linked mutations result in increased production of the longer form of amyloid-beta (main component of amyloid deposits found in AD brains). Presenilins are postulated to regulate APP processing through their effects on gamma-secretase, an enzyme that cleaves APP. Also, it is thought that the presenilins are involved in the cleavage of the Notch receptor such that, they either directly regulate gamma-secretase activity, or themselves act are protease enzymes. Two alternatively spliced transcript variants encoding different isoforms of PSEN2 have been identified.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Abrogation of the presenilin 1/beta-catenin interaction and preservation of the heterodimeric presenilin 1 complex following caspase activation.

The Journal of biological chemistry

Tesco G,Kim TW,Diehlmann A,Beyreuther K,Tanzi RE

MA1-754 was used in immunoprecipitation to investigate the impacts of caspase activation on heterodimeric presenilin 1 complex and the interaction between PS1 and beta-catenin