Invitrogen

PTCH2 Polyclonal Antibody

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PTCH2 Antibody (PA1-46223) in WB

Western blot analysis of PTCH2 in fetal brain lysate. Samples were incubated in PTCH2 polyclonal antibody (Product # PA1-46223) using a dilution of 1:500.

PTCH2 Antibody in Immunocytochemistry (ICC/IF)

PTCH2 Antibody in Western Blot, Immunoprecipitation (WB, IP)

Product Image Gallery

PTCH2 Antibody — Figure 3. Abeta 1-42 not Abeta 1-40 increases the expressions of Shh signaling in hippocampal GPCs derived from WT mice. The suspended cells were incubated with the presence of 0, 0.1, 1, 10 or 100 u m of Abeta 1-42 or Abeta 1-40 for 3 days. ( A ) Shh expression with different molecular weights was shown by western blot from the cultured medium and the cells treated with Abeta 1-42 . ( B ) Shh levels were significantly increased in the conditioned medium (normalized to corresponding cell lysate) and cultured cells in a dose-dependent manner with Abeta 1-42 treatment (ANOVA test, * P < 0.05, **P < 0.01). ( C ) The expression of Shh signaling components was shown in the cells with Abeta 1-42 treatment. ( D ) The levels of Ptc1, Ptc2, Smo, Gli1 and Gli2 were significant elevated (ANOVA test, * P < 0.05, * * P < 0.01) but not significant changes of Gli3 levels with the exposure of Abeta 1-42 (ANOVA test, P > 0.05). ( E ) Shh expression was shown from the conditioned medium and GPCs treated with Abeta 1-40 . ( F ) Shh levels were not significantly increased in the conditioned medium (normalized to corresponding cell lysate) and the cultured GPCs with the Abeta 1-40 treatment (ANOVA test, P > 0.05). ( G ) The expression of Shh signaling component molecules was shown in the GPC lysate with the Abeta 1-40 treatment. ( H ) The levels of the components were not significantly changed with the Abeta 1-40 treatment. ( I ) Western blot showed Ptc1 expression with the exogenous Shh applicat {TM}

产品信息

PA1-46223

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:1,000

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免疫组化 (IHC)

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免疫组化（石蜡） (IHC (P))

1:10-1:500

免疫组化（冰冻） (IHC (F))

1:10-1:500

免疫细胞化学 (ICC/IF)

1:10-1:500

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酶联免疫吸附实验 (ELISA)

1:100-1:2,000

免疫沉淀 (IP)

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产品规格

种属反应

Human, Mouse

已发表种属

Mouse

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Residues 226-235 of the human PTCH2 protein

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS

内含物

0.02% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Ambient (domestic); Wet ice (international)

AB_2174052

靶标信息

Mutations of the human Patched tumor suppressor gene (PTCH) have been identified in individuals with the nevoid basal cell carcinoma syndrome (NBCCS) as well as in sporadic basal cell carcinomas and medulloblastomas. PTCH2 has a frameshift mutation resulting in a truncated PTCH2 protein.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Coordination of canonical and noncanonical Hedgehog signalling pathways mediated by WDR11 during primordial germ cell development.

Scientific reports

Lee J,Kim Y,Ataliotis P,Kim HG,Kim DW,Bennett DC,Brown NA,Layman LC,Kim SH

PA1-46223 was used in Western Blotting to provide evidence supporting a role for WDR11 in ciliogenesis through regulation of anterograde intraflagellar transport potentially via its interaction with IFT20.

Sat Jul 29 00:00:00 UTC 2023

Coordination of canonical and noncanonical Hedgehog signalling pathways mediated by WDR11 during primordial germ cell development.

Scientific reports

Lee J,Kim Y,Ataliotis P,Kim HG,Kim DW,Bennett DC,Brown NA,Layman LC,Kim SH

Sat Jul 29 00:00:00 UTC 2023

Ptch2/Gas1 and Ptch1/Boc differentially regulate Hedgehog signalling in murine primordial germ cell migration.

Nature communications

Kim Y,Lee J,Seppala M,Cobourne MT,Kim SH

PA1-46223 was used in Immunohistochemistry-immunofluorescence to identify a divergent Hedgehog signal pathway mediated by Ptch2 and provide a mechanism for differential interpretation of Hedgehog signalling in the germ cell niche.