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Phospho-BAD (Ser26) Antibody (PA5-143655) in WB
Western blot of Phospho-BAD (Ser26) in mouse J774A.1 macrophage stimulated with calyculin A (lanes 1-4) then dephosphorylated with lambda phosphatase (lanes 2 & 4). The blots were probed with mouse anti-Bad monoclonal antibody (lanes 1 & 2), and rabbit Phospho-BAD (Ser26) polyclonal antibody (Product # PA5-143655) (lanes 3 & 4).
产品信息
PA5-143655
产品规格
种属反应
Mouse
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Phospho-Bad (Ser-26) synthetic peptide (coupled to KLH) corresponding to amino acid residues surrounding serine 26 in mouse Bad. This peptide sequence is highly conserved in rat Bad, but is not found in human Bad.
偶联物
形式
Liquid
浓度
0.1 mg/mL
纯化类型
Antigen affinity chromatography
保存液
PBS with 1mg/mL BSA, 50% glycerol
内含物
0.05% sodium azide
保存条件
-20° C, Avoid Freeze/Thaw Cycles
运输条件
Wet ice
RRID
AB_2942883
产品详细信息
The antibody detects a 23 kDa* doublet corresponding to the apparent molecular mass of phosphorylated Bad on SDS-PAGE immunoblots of mouse J774A.1 treated with calyculin A. This reactivity is not observed after lambda phosphatase treatment.
靶标信息
The protein encoded by BAD is a member of the BCL-2 family. BCL-2 family members are known to be regulators of programmed cell death. This protein positively regulates cell apoptosis by forming heterodimers with BCL-xL and BCL-2, and reversing their death repressor activity. Proapoptotic activity of this protein is regulated through its phosphorylation. Protein kinases AKT and MAP kinase, as well as protein phosphatase calcineurin were found to be involved in the regulation of this protein. Alternative splicing of this gene results in two transcript variants which encode the same isoform.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: BAD; Bcl-2-binding component 6; Bcl-xL/Bcl-2-associated death promoter; Bcl2 antagonist of cell death; Bcl2-associated agonist of cell death; OTTMUSP00000017561; OTTMUSP00000022400
基因别名: AI325008; Bad; Bbc2; Bbc6
UniProt ID: (Mouse) Q61337
Entrez Gene ID: (Mouse) 12015
release of cytochrome c from mitochondria
glucose catabolic process
apoptotic process
activation of cysteine-type endopeptidase activity involved in apoptotic process
cell proliferation
extrinsic apoptotic signaling pathway via death domain receptors
intrinsic apoptotic signaling pathway in response to DNA damage
positive regulation of mitochondrial membrane potential
suppression by virus of host apoptotic process
cytokine-mediated signaling pathway
positive regulation of insulin secretion
positive regulation of glucokinase activity
positive regulation of insulin secretion involved in cellular response to glucose stimulus
glucose homeostasis
regulation of apoptotic process
positive regulation of apoptotic process
positive regulation of cysteine-type endopeptidase activity involved in apoptotic process
regulation of cysteine-type endopeptidase activity involved in apoptotic process
type B pancreatic cell proliferation
positive regulation of B cell differentiation
positive regulation of T cell differentiation
positive regulation of proteolysis
negative regulation of cytolysis
ADP metabolic process
ATP metabolic process
regulation of mitochondrial membrane permeability
pore complex assembly
positive regulation of epithelial cell proliferation
positive regulation of apoptotic process by virus
cellular process regulating host cell cycle in response to virus
cellular response to organic substance
cellular response to nicotine
positive regulation of release of cytochrome c from mitochondria
apoptotic signaling pathway
extrinsic apoptotic signaling pathway
extrinsic apoptotic signaling pathway in absence of ligand
intrinsic apoptotic signaling pathway
activation of cysteine-type endopeptidase activity
positive regulation of neuron death
positive regulation of type B pancreatic cell development
