Invitrogen

Phospho-Cortactin (Tyr421) Polyclonal Antibody

Phospho-Cortactin (Tyr421) Antibody in Immunocytochemistry (ICC/IF)

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Phospho-Cortactin (Tyr421) Antibody (44-854G) in ICC/IF

Immunofluorescence analysis of Phospho-Cortactin (Tyr421) was performed using 70% confluent log phase NIH/3T3 cells treated with 1.6 mM hydrogen peroxide for 1 h. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Phospho-Cortactin (Tyr421) Rabbit Polyclonal An... View More

Phospho-Cortactin (Tyr421) Antibody in Western Blot (WB)

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产品信息

44-854G

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000

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免疫细胞化学 (ICC/IF)

1:250

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其他PubMed (Misc)

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产品规格

种属反应

Human, Mouse

已发表种属

Bovine, Human, Mouse

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

The antiserum was produced against a chemically synthesized phosphopeptide derived from the region of mouse cortactin that contains tyrosine 421.

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

纯化类型

Antigen affinity chromatography

保存液

Dulbecco's PBS, pH 7.3, with 1mg/mL BSA

内含物

0.05% sodium azide

保存条件

-20°C

运输条件

Ambient (domestic); Wet ice (international)

AB_2533780

靶标信息

Cortactin (Cttn) is a ubiquitous actin-binding protein that was originally identified as a substrate for Src. It contributes to the organization of the actin cytoskeleton and cell shape. Cortactin also plays a role in the formation of lamellipodia and cell migration. It is accumulated in peripheral, actin-enriched structures of cells suggesting that cortactin facilitates actin network formation. Cortactin has four major domains of interest the N-terminal acidic (NTA) and tandem repeats domains and the C-terminal proline-rich and SH3 Domains. NTA associates with the Arp2/3 and WASP complex at F-actin branches. Cortactin is involved in promoting cell motility and invasion, including a critical role in invadopodia, actin rich-subcellular protrusions associated with degradation of the ECM by cancer cells. Cortactin is phosphorylated by src family kinases at Y421, Y466, and Y482 and S405 and S418 that are phosphorylated by Erk family kinases.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Pressure and stiffness sensing together regulate vascular smooth muscle cell phenotype switching.

Science advances

Swiatlowska P,Sit B,Feng Z,Marhuenda E,Xanthis I,Zingaro S,Ward M,Zhou X,Xiao Q,Shanahan C,Jones GE,Yu CH,Iskratsch T

44-854G was used in Western Blotting to show how different pathological mechanical signals combined but through distinct pathways accelerate a phenotypic switch that will ultimately contribute to atherosclerotic disease progression.

Fri Apr 15 00:00:00 UTC 2022

Pressure and stiffness sensing together regulate vascular smooth muscle cell phenotype switching.

Science advances

Swiatlowska P,Sit B,Feng Z,Marhuenda E,Xanthis I,Zingaro S,Ward M,Zhou X,Xiao Q,Shanahan C,Jones GE,Yu CH,Iskratsch T

Fri Apr 15 00:00:00 UTC 2022

Tumor necrosis factor α-mediated restructuring of the Sertoli cell barrier in vitro involves matrix metalloprotease 9 (MMP9), membrane-bound intercellular adhesion molecule-1 (ICAM-1) and the actin cytoskeleton.

Spermatogenesis

Lydka M,Bilinska B,Cheng CY,Mruk DD

44-854G was used in western blot to examine the effects of TNFalpha on Sertoli cell barrier function

Mon Oct 01 00:00:00 UTC 2012

Doubles game: Src-Stat3 versus p53-PTEN in cellular migration and invasion.

Molecular and cellular biology

Mukhopadhyay UK,Mooney P,Jia L,Eves R,Raptis L,Mak AS

44-854G was used in immunocytochemistry to explore the mechanism by which Src and p53 play antagonistic roles in cell invasion

Mon Nov 01 00:00:00 UTC 2010

p53 suppresses Src-induced podosome and rosette formation and cellular invasiveness through the upregulation of caldesmon.

Molecular and cellular biology

Mukhopadhyay UK,Eves R,Jia L,Mooney P,Mak AS

44-854G was used in immunocytochemistry to elucidate how p53 modulates cell migration and invasion

Mon Jun 01 00:00:00 UTC 2009

Paxillin-Y118 phosphorylation contributes to the control of Src-induced anchorage-independent growth by FAK and adhesion.

BMC cancer

Sachdev S,Bu Y,Gelman IH

44-854G was used in western blot to review the functional consequences of interactions between focal adhesion kinase and Src are protein tyrosine kinases

Mon Jan 12 00:00:00 UTC 2009

Formation of extracellular matrix-digesting invadopodia by primary aortic smooth muscle cells.

Circulation research

Furmaniak-Kazmierczak E,Crawley SW,Carter RL,Maurice DH,Côté GP

44-854G was used in immunocytochemistry to identify factors that induce vascular smooth muscle cells to form invadopodia

Fri May 11 00:00:00 UTC 2007

Crk-associated substrate tyrosine phosphorylation sites are critical for invasion and metastasis of SRC-transformed cells.

Molecular cancer research : MCR

Brábek J,Constancio SS,Siesser PF,Shin NY,Pozzi A,Hanks SK

Wed Jun 01 00:00:00 UTC 2005

Cortactin tyrosine phosphorylation requires Rac1 activity and association with the cortical actin cytoskeleton.

Molecular biology of the cell

Head JA,Jiang D,Li M,Zorn LJ,Schaefer EM,Parsons JT,Weed SA

Fri Aug 01 00:00:00 UTC 2003

生物信息学

蛋白别名: 1110020L01Rik; Amplaxin; ems1 sequence (mammary tumor and squamous cell carcinoma-associated (p80/85 src substrate); FLJ34459; mammary tumor and squamous cell carcinoma associated (p80/85 src substrate); Oncogene EMS1; Oncogene SRC8; Src substrate cortactin; src8

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基因别名: 1110020L01Rik; CTTN; EMS1

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UniProt ID: (Human) Q14247, (Mouse) Q60598

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Entrez Gene ID: (Human) 2017, (Mouse) 13043

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功能

protein binding profilin binding cadherin binding involved in cell-cell adhesion proline-rich region binding actin or actin-binding cytoskeletal protein cytoskeletal protein non-motor actin binding protein

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