Invitrogen

Phospho-IRS1 (Ser616) Polyclonal Antibody

Phospho-IRS1 (Ser616) Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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Phospho-IRS1 (Ser616) Antibody (44-550G) in IHC (P)

Immunohistochemistry analysis of Phospho-IRS1 (pS616) showing staining in the nucleus of paraffin-embedded human breast carcinoma tissue (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min ... View More

Phospho-IRS1 (Ser616) Antibody in Western Blot (WB)

Phospho-IRS1 (Ser616) Antibody in Western Blot, Immunocytochemistry (WB, ICC/IF)

Phospho-IRS1 (Ser616) Antibody in Immunocytochemistry, Immunohistochemistry, Immunohistochemistry (Paraffin) (ICC/IF, IHC, IHC (P))

Phospho-IRS1 (Ser616) Antibody in Immunohistochemistry, Immunohistochemistry (Paraffin) (IHC, IHC (P))

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产品信息

44-550G

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000

查看5篇已发表文章 5篇已发表文章

免疫组化 (IHC)

查看2篇已发表文章 2篇已发表文章

免疫组化（石蜡） (IHC (P))

1:10-1:100

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免疫细胞化学 (ICC/IF)

查看2篇已发表文章 2篇已发表文章

免疫沉淀 (IP)

查看1篇已发表文章 1篇已发表文章

其他PubMed (Misc)

查看1篇已发表文章 1篇已发表文章

产品规格

种属反应

Human, Mouse, Rat

已发表种属

Human, Mouse

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

The antiserum was produced against a chemically synthesized phosphopeptide derived from a region of IRS-1 that contains serine 616. The sequence is conserved in many species including human, mouse, and rat

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

纯化类型

Antigen affinity chromatography

保存液

Dulbecco's PBS, pH 7.3, with 1mg/mL BSA

内含物

0.05% sodium azide

保存条件

-20°C

运输条件

Ambient (domestic); Wet ice (international)

AB_2533678

产品详细信息

Purified from rabbit serum by sequential epitope-specific chromatography, this product contains enough material for 10 mini-blots. This antibody has been negatively preadsorbed using a non-phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with non-phosphorylated insulin receptor substrate-1 (IRS-1). The final product is generated by affinity chromatography using IRS-1 derived peptide that is phosphorylated at serine 616.

The antibody has been used in western blotting. Previous lots of this antibody have been used in immunohistochemistry. In western analysis the positive control used was human embryonic kidney cell line transfected with a vector encoding IRS-1 (293T) and stimulated with a phorbol ester, TPA.

靶标信息

IRS-1, a major substrate of the insulin receptor, is phosphorylated in response to stimulation of cells by insulin, insulin-like growth factor 1 (IGF-1) and interleukin 4 (IL-4). IRS-1 is phosphorylated on serine, threonine and tyrosine residues in a variety of tissues. An insulin-sensitive serine/threonine kinase casein kinase II mediates a portion of the insulin-stimulated serine/threonine phosphorylation of overexpressed IRS-1 in vivo. Thr 502 is identified as the major casein kinase II-catalyzed phosphorylation site in rat IRS-1, and Ser 99 is an additional phosphorylation site catalyzed by casein kinase II. Thus, casein kinase II-catalyzed phosphorylation of IRS-1 may be a component of the intracellular insulin signaling cascade. IRS-1 contains three putative binding sites for 14-3-3 (Ser 270, Ser 374 and Ser 641) and the motif around Ser 270 is located in the phosphortyrosine binding domain of IRS-1, which is responsible for the interaction with the insulin receptor.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Brain Insulin Signaling is Associated with Late-Life Cognitive Decline.

Aging and disease

Tong H,Capuano AW,Carmichael OT,Gwizdala KL,Bennett DA,Ahima RS,Arnold SE,Arvanitakis Z

44-550G was used in Immunohistochemistry to provide novel evidence for an association between brain insulin signaling and late-life cognitive decline.

Tue Oct 01 00:00:00 UTC 2024

Induction of Brain Insulin Resistance and Alzheimer's Molecular Changes by Western Diet.

International journal of molecular sciences

Mietelska-Porowska A,Domańska J,Want A,Więckowska-Gacek A,Chutorański D,Koperski M,Wojda U

44-550G was used in Western Blotting to suggest that WD promotes the development of AD and may be considered not only a risk factor, but also a modifiable trigger of AD.

Mon Apr 25 00:00:00 UTC 2022

Deletion of serine racemase reverses neuronal insulin signaling inhibition by amyloid-β oligomers.

Journal of neurochemistry

Zhou J,Zhang Z,Yang Y,Liao F,Zhou P,Wang Y,Zhang H,Jiang H,Alinejad T,Shan G,Wu S

44-550G was used in Western Blot to enhance insulin signaling which is inhibited at the early stage of the AD brain.

Sat Oct 01 00:00:00 UTC 2022

Altered Insulin Receptor Substrate 1 Phosphorylation in Blood Neuron-Derived Extracellular Vesicles From Patients With Parkinson's Disease.

Frontiers in cell and developmental biology

Chou SY,Chan L,Chung CC,Chiu JY,Hsieh YC,Hong CT

44-550G was used in Immunoprecipitation to suggest the association between dysinsulin signaling pathway with PD.

Sat Nov 04 00:00:00 UTC 2023

Central insulin dysregulation and energy dyshomeostasis in two mouse models of Alzheimer's disease.

Neurobiology of aging

Velazquez R,Tran A,Ishimwe E,Denner L,Dave N,Oddo S,Dineley KT

44-550G was used in Western Blotting to probe for peripheral insulin resistance, central nervous system insulin sensitivity and energy homeostasis in Tg2576 and 3xTg-Alzheimer's disease mice.

Sun Oct 01 00:00:00 UTC 2017

Insulin and IGF1 signalling pathways in human astrocytes in vitro and in vivo; characterisation, subcellular localisation and modulation of the receptors.

Molecular brain

Garwood CJ,Ratcliffe LE,Morgan SV,Simpson JE,Owens H,Vazquez-Villaseñor I,Heath PR,Romero IA,Ince PG,Wharton SB

44-550G was used in western blot to study the insulin/IGF1 signaling pathway in human astrocytes.

Sat Aug 22 00:00:00 UTC 2015

Insulin and IGF1 signalling pathways in human astrocytes in vitro and in vivo; characterisation, subcellular localisation and modulation of the receptors.

Molecular brain

Garwood CJ,Ratcliffe LE,Morgan SV,Simpson JE,Owens H,Vazquez-Villaseñor I,Heath PR,Romero IA,Ince PG,Wharton SB

44-550G was used in western blot to study the insulin/IGF1 signaling pathway in human astrocytes.

Sat Aug 22 00:00:00 UTC 2015

Insulin and IGF1 signalling pathways in human astrocytes in vitro and in vivo; characterisation, subcellular localisation and modulation of the receptors.

Molecular brain

Garwood CJ,Ratcliffe LE,Morgan SV,Simpson JE,Owens H,Vazquez-Villaseñor I,Heath PR,Romero IA,Ince PG,Wharton SB

44-550G was used in western blot to study the insulin/IGF1 signaling pathway in human astrocytes.

Sat Aug 22 00:00:00 UTC 2015

Abnormal serine phosphorylation of insulin receptor substrate 1 is associated with tau pathology in Alzheimer's disease and tauopathies.

Acta neuropathologica

Yarchoan M,Toledo JB,Lee EB,Arvanitakis Z,Kazi H,Han LY,Louneva N,Lee VM,Kim SF,Trojanowski JQ,Arnold SE

44-550G was used in immunohistochemistry - paraffin section to determine the expression of abnormal serine phosphorylation of insulin receptor substrate 1 in healthy and diseased human brains