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Invitrogen
Phospho-PAK1/2/3 (Ser144, Ser141, Ser139) Recombinant Rabbit Monoclonal Antibody (SU03-70)
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Phospho-PAK1/2/3 (Ser144, Ser141, Ser139) Antibody (MA5-32130) in ICC/IF
Immunocytochemical analysis of Phospho-PAK1/2/3 (Ser144, Ser141, Ser139) in NIH/3T3 cells using a Phospho-PAK1/2/3 (Ser144, Ser141, Ser139) Monoclonal antibody (Product # MA5-32130) as seen in green. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
产品信息
MA5-32130
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse,
Rat
宿主/亚型
Rabbit
/ IgG
Expression System
HEK293 cells
分类
Recombinant Monoclonal
类型
Antibody
克隆号
SU03-70
抗原
Synthetic phospho-peptide corresponding to residues surrounding Ser144 of Human PAK1 aa 121-150
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Protein A
保存液
TBS, pH 7.4, with 40% Glycerol, 0.05% BSA
内含物
0.05% sodium azide
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Wet ice
RRID
AB_2809421
产品详细信息
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
靶标信息
In mammals, there are several identified isoforms of p21-Activated Protein Kinases or PAKs: alpha-PAK (also known as PAK-1) and beta-PAK (also known as PAK-3) are mostly brain-specific, while gamma-PAK (also known as PAK-2) is expressed ubiquitously (Jakobi et al., 2003). Mutations of the gene coding for PAK-3 are associated wit X-linked mental retardation and recent work indicates that PAK-3 is a key regulator of synapse formation and plasticity in the hippocampus (Boda et al., 2004). PAK-3 is thought to play a key role in regulation of cell shape and motility as well as cell death (Jakobi et al., 2003; Walter et al., 1998). Autophosphorylation of Thr402 in the protein has been found to be essential for activation of PAK (Jakobi et al., 2000).
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: activated protein kinase alpha; ADRB2; adriamycin resistance-associated; Alpha-PAK; Beta-PAK; CDC42/RAC effector kinase PAK-A; CDC42/RAC effector kinase PAK-B; CDKN1A; EC 2.7.11.1; gamma pak; Gamma-PAK; hPAK3; kinase PAK1; Oligophrenin-3; p21 (CDKN1A)-activated kinase 1; p21 (CDKN1A)-activated kinase 2; p21 (CDKN1A)-activated kinase 3; p21 protein (Cdc42/Rac)-activated kinase 1; p21 protein (Cdc42/Rac)-activated kinase 2; p21 protein (Cdc42/Rac)-activated kinase 3; p21-activated kinase 1; p21-activated kinase 2; p21-activated kinase 3; p21/Cdc42/Rac1-activated kinase 1 (STE20 homolog, yeast); p21/Cdc42/Rac1-activated kinase 1 (yeast Ste20-related); p21/Cdc42/Rac1-activated kinase 3 (yeast Ste20-related); p58; p65-PAK; p68-PAK; PAK 1; PAK alpha; PAK beta; PAK gamma; PAK-1; PAK-2; PAK-3; PAK65; Protein kinase MUK2; S6/H4 kinase; Serine/threonine-protein kinase PAK 1; Serine/threonine-protein kinase PAK 2; Serine/threonine-protein kinase PAK 3; STE20 homolog, yeast
基因别名: 5330420P17Rik; A130002K10Rik; AI836325; ARA; AW045634; beta-PAK; bPAK; D16Ertd269e; gamma-PAK; mKIAA4182; MRX30; MRX47; OPHN3; PAK-1; PAK-2; PAK-3; PAK1; PAK2; PAK3; PAK3beta; PAK65; Pak65alpha; Pak65beta; Paka; PAKalpha; Pakb; PAKgamma; Stk4
UniProt ID: (Human) Q13153, (Human) Q13177, (Human) O75914, (Rat) P35465, (Mouse) Q8CIN4, (Rat) Q64303, (Mouse) O88645, (Rat) Q62829
Entrez Gene ID: (Human) 5058, (Human) 5062, (Human) 5063, (Mouse) 18479, (Rat) 29431, (Mouse) 224105, (Rat) 29432, (Mouse) 18481, (Rat) 29433
protein kinase activity
protein serine/threonine kinase activity
receptor signaling protein serine/threonine kinase activity
protein binding
collagen binding
ATP binding
protein kinase binding
protein tyrosine kinase activator activity
identical protein binding
cadherin binding involved in cell-cell adhesion
MAP kinase kinase activity
Rho GTPase binding
SH3 domain binding
metal ion binding
nucleotide binding
catalytic activity
kinase activity
transferase activity
transferase activity, transferring phosphorus-containing groups
Rac GTPase binding
MAP kinase kinase kinase activity
GTPase binding
non-receptor serine/threonine protein kinase
MAPK cascade
mitotic cell cycle
response to hypoxia
positive regulation of protein phosphorylation
stimulatory C-type lectin receptor signaling pathway
protein phosphorylation
exocytosis
apoptotic process
axonogenesis
positive regulation of cell proliferation
positive regulation of cell migration
T cell costimulation
actin cytoskeleton reorganization
cellular response to insulin stimulus
positive regulation of peptidyl-serine phosphorylation
positive regulation of intracellular estrogen receptor signaling pathway
Fc-epsilon receptor signaling pathway
Fc-gamma receptor signaling pathway involved in phagocytosis
wound healing
positive regulation of JUN kinase activity
protein autophosphorylation
ephrin receptor signaling pathway
branching morphogenesis of an epithelial tube
neuron projection morphogenesis
T cell receptor signaling pathway
positive regulation of stress fiber assembly
negative regulation of cell proliferation involved in contact inhibition
negative regulation of protein kinase activity
signal transduction
viral process
phosphorylation
peptidyl-serine phosphorylation
signal transduction by protein phosphorylation
regulation of growth
negative regulation of apoptotic process
vascular endothelial growth factor receptor signaling pathway
regulation of defense response to virus by virus
positive regulation of peptidyl-tyrosine phosphorylation
dendritic spine development
positive regulation of protein tyrosine kinase activity
cellular response to organic cyclic compound
cell-cell adhesion
positive regulation of extrinsic apoptotic signaling pathway
negative regulation of cysteine-type endopeptidase activity involved in execution phase of apoptosis
activation of MAPK activity
dendrite development
regulation of actin filament polymerization
positive regulation of neuron apoptotic process
synapse organization
dendritic spine morphogenesis
positive regulation of dendritic spine morphogenesis
positive regulation of DNA biosynthetic process
Rho protein signal transduction
regulation of mitotic cell cycle
neuromuscular junction development
metabolic process
response to organic substance
regulation of gene expression
cell migration
amygdala development
actin cytoskeleton organization
stress-activated protein kinase signaling cascade
activation of protein kinase activity
regulation of actin cytoskeleton organization
regulation of apoptotic process
receptor clustering
regulation of MAPK cascade
gamma-aminobutyric acid secretion, neurotransmission
glutamate secretion, neurotransmission
observational learning
regulation of long-term synaptic potentiation
activation of MAPKK activity
small GTPase mediated signal transduction
intracellular signal transduction
multicellular organism development
regulation of neuron projection development
