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Phospho-PI 3 Kinase p85 alpha (Tyr607) Polyclonal Antibody

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说明书
实验方案
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产品信息

600-401-MR1

应用
建议稀释比
已发表文章

免疫印迹 (WB)

Assay-dependent
-

免疫组化 (IHC)

1:100
-

酶联免疫吸附实验 (ELISA)

1:10,000-1:50,000
-

点杂交 (DB)

1:70,000
-
产品规格

种属反应

Human

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Anti-PI 3 Kinase p85 alpha pY607 antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a C-Terminal portion of human PIK3R1 conjugated to Keyhole Limpet Hemocyanin (KLH).
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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.8 mg/mL

纯化类型

Affinity chromatography

保存液

0.02M potassium phosphate, pH 7.2, with 0.15M NaCl

内含物

0.01% sodium azide

保存条件

-20° C, Avoid Freeze/Thaw Cycles

运输条件

Ambient (domestic); Wet ice (international)

产品详细信息

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

This affinity purified antibody is directed against the phosphorylated form of human PIK3R1 protein at the pY607 residue. Reactivity with non-phosphorylated human PIK3R1 Y607 is minimal by ELISA and dot blot. Reactivity against homologues from other sources is not known. A BLAST analysis was used to suggest reactivity with the antigen based on 100% homology with the immunizing sequence to human, mouse, and rat.

靶标信息

Phosphatidylinositol 3-kinase (PIK3) is a signaling protein that phosphorylates the inositol ring of phosphatidylinositol at the 3-prime position. Signaling is initiated when upstream tyrosine kinase receptors or G protein coupled receptors are bound by their ligands. It binds to activated (phosphorylated) protein-Tyr kinases, through its SH2 domain, and acts as an adapter, mediating the association of the p110 catalytic unit to the plasma membrane. Phosphatidylinositol 3-kinase plays an important role in the metabolic actions of insulin, and a mutation in this gene has been associated with insulin resistance. During insulin stimulation, it also binds to IRS-1. Mutations in the gene can result in Agammaglobulinemia 7, SHORT syndrome, or Immunodeficiency 36.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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生物信息学

蛋白别名: p55-GAMMA; p55PIK; phosphatidylinositol 3-kinase 55 kDa regulatory subunit gamma; Phosphatidylinositol 3-kinase 85 kDa regulatory subunit alpha; Phosphatidylinositol 3-kinase regulatory subunit alpha; phosphatidylinositol 3-kinase regulatory subunit gamma; phosphatidylinositol 3-kinase, regulatory subunit, polypeptide 1 (p85 alpha); phosphatidylinositol 3-kinase-associated p-85 alpha; phosphoinositide-3-kinase regulatory subunit; phosphoinositide-3-kinase regulatory subunit alpha; phosphoinositide-3-kinase, regulatory subunit 1 (alpha); PI3-kinase p85-subunit alpha; PI3-kinase regulatory subunit alpha; PI3-kinase regulatory subunit gamma; PI3-kinase subunit p55-gamma; PI3-kinase subunit p85-alpha; PI3K regulatory subunit alpha; PI3K regulatory subunit gamma; polypeptide 1 p85 alpha; ptdIns-3-kinase regulatory subunit alpha; PtdIns-3-kinase regulatory subunit p85-al; RP4-533D7.2

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基因别名: AGM7; GRB1; IMD36; p85; p85-ALPHA; PIK3R1

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UniProt ID: (Human) P27986

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Entrez Gene ID: (Human) 5295

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功能
transmembrane receptor protein tyrosine kinase adaptor activity insulin receptor binding insulin-like growth factor receptor binding neurotrophin TRKA receptor binding protein binding transcription factor binding 1-phosphatidylinositol-3-kinase activity protein phosphatase binding phosphatidylinositol 3-kinase regulator activity phosphatidylinositol 3-kinase regulatory subunit binding ErbB-3 class receptor binding phosphatidylinositol 3-kinase binding insulin binding insulin receptor substrate binding phosphatidylinositol-4,5-bisphosphate 3-kinase activity 1-phosphatidylinositol-3-kinase regulator activity protein heterodimerization activity kinase modulator
参与通路
cellular glucose homeostasis negative regulation of cell-matrix adhesion protein phosphorylation phosphatidylinositol biosynthetic process epidermal growth factor receptor signaling pathway insulin receptor signaling pathway extrinsic apoptotic signaling pathway via death domain receptors intrinsic apoptotic signaling pathway in response to DNA damage phosphatidylinositol 3-kinase signaling regulation of phosphatidylinositol 3-kinase signaling viral process platelet activation B cell differentiation positive regulation of cell migration T cell costimulation positive regulation of tumor necrosis factor production cellular response to insulin stimulus positive regulation of RNA splicing cellular response to UV response to endoplasmic reticulum stress phosphatidylinositol-3-phosphate biosynthetic process Fc-epsilon receptor signaling pathway Fc-gamma receptor signaling pathway involved in phagocytosis ERBB2 signaling pathway positive regulation of transcription factor import into nucleus negative regulation of apoptotic process regulation of phosphatidylinositol 3-kinase activity negative regulation of osteoclast differentiation positive regulation of transcription from RNA polymerase II promoter positive regulation of glucose import phosphatidylinositol phosphorylation insulin-like growth factor receptor signaling pathway vascular endothelial growth factor receptor signaling pathway phosphatidylinositol-mediated signaling protein stabilization T cell receptor signaling pathway leukocyte migration regulation of stress fiber assembly NFAT protein import into nucleus growth hormone receptor signaling pathway positive regulation of establishment of protein localization to plasma membrane positive regulation of endoplasmic reticulum unfolded protein response positive regulation of glucose import in response to insulin stimulus
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