Invitrogen

Phospho-SMAD1/SMAD5 (Ser463, Ser465) Recombinant Rabbit Monoclonal Antibody (31H14L11)

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产品信息

700047

应用
建议稀释比
已发表文章

免疫印迹 (WB)

1-2 µg/mL
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免疫组化 (IHC)

-
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免疫组化（石蜡） (IHC (P))

4-6 µg/mL
-

免疫细胞化学 (ICC/IF)

2-4 µg/mL
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流式细胞分析 (Flow)

0.5-5 µg/1x10^6 cells
-

其他PubMed (Misc)

-
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产品规格

种属反应

Human

已发表种属

Fish, Mammal, Mouse

宿主/亚型

Rabbit / IgG

Expression System

Expi293

分类

Recombinant Monoclonal

类型

Antibody

克隆号

31H14L11

抗原

A peptide corresponding to amino acids 456-465 of Q15797 and Q99717.
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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Protein A

保存液

PBS

内含物

0.09% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

RRID

AB_2532276

产品详细信息

This antibody is predicted to react with mouse, primate, rat, Rhesus monkey , sea urchin, ovine, porcine, Xenopus and zebrafish based on sequence homology.

Intact IgG appears on a non-reducing gel as ~150 kDa band and upon reduction generating a ~25 kDa light chain band and a ~50 kDa heavy chain.

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

靶标信息

The Smad family of proteins are functioning in the transmission of extracellular signals in the TGF-beta signaling pathway. Binding of a TGF-beta superfamily ligands to extracellular receptors triggers phosphorylation of Smad2 at a Serine-Serine-Methionine-Serine (SSMS) motif at its C-terminus. Phosphorylated Smad2 is then able to form a complex with Smad4. These complexes accumulate in the cell nucleus, where they are directly participating in the regulation of gene expression. In mammals, eight Smad proteins have been identified to date. The Smad family of proteins can be divided into three functional groups: the receptor-activated Smads (R-Smads), common mediator Smads (Co-Smads), and the inhibitory Smads (I-Smads). The R-Smads are directly phosphorylated by the activated type I receptors on their C-terminal Ser-Ser-X-Ser (SSXS) motif and include Smad1, Smad2, Smad3, Smad5, and Smad8. Smad2 and Smad3 are phosphorylated in response to TGF-beta and activin, whereas Smad1, Smad5, and Smad8 are phosphorylated in response to BMP (Bone Morphogenetic Protein). This C-terminal phosphorylation allows R-Smad binding to Co-Smad, Smad4, and translocation to the nucleus where they regulate TGF-beta target genes. Smad6 and Smad7 belong to the I-Smad which bind to the type I receptor or Smad4 and block their interaction with R-Smads.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

生物信息学

蛋白别名: BSP-1; hSMAD1; hSmad5; JV4-1; JV5-1; MAD homolog 1; MAD homolog 5; MAD, mothers against decapentaplegic homolog 1; MAD, mothers against decapentaplegic homolog 5; Mad-related protein 1; Mothers against decapentaplegic homolog 1; Mothers against decapentaplegic homolog 5; mothers against decapentaplegic, drosophila, homolog of, 5; mothers against DPP homolog 1; OTTHUMP00000223331; SMA- and MAD-related protein 5; SMAD; SMAD 1; SMAD 5; SMAD family member 1; SMAD family member 5; SMAD, mothers against DPP homolog 1; SMAD, mothers against DPP homolog 5; TGF-beta signaling protein 1; transforming growth factor-beta signaling protein 1; Transforming growth factor-beta-signaling protein 1

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基因别名: BSP-1; BSP1; DWFC; JV4-1; JV41; JV5-1; MADH1; MADH5; MADR1; SMAD1; SMAD5

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UniProt ID: (Human) Q15797, (Human) Q99717

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Entrez Gene ID: (Human) 4086, (Human) 4090

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功能
RNA polymerase II core promoter proximal region sequence-specific DNA binding RNA polymerase II core promoter sequence-specific DNA binding transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding transcription factor activity, sequence-specific DNA binding receptor signaling protein activity protein binding protein kinase binding transforming growth factor beta receptor, pathway-specific cytoplasmic mediator activity identical protein binding protein homodimerization activity metal ion binding protein heterodimerization activity co-SMAD binding I-SMAD binding ubiquitin protein ligase binding DNA-binding transcription factor gene-specific transcriptional regulator
参与通路
MAPK cascade ureteric bud development mesodermal cell fate commitment osteoblast fate commitment transcription from RNA polymerase II promoter inflammatory response signal transduction transforming growth factor beta receptor signaling pathway SMAD protein complex assembly gamete generation negative regulation of cell proliferation embryonic pattern specification positive regulation of gene expression BMP signaling pathway midbrain development hindbrain development primary miRNA processing homeostatic process positive regulation of osteoblast differentiation positive regulation of transcription from RNA polymerase II promoter cartilage development cardiac muscle cell proliferation bone development SMAD protein signal transduction positive regulation of cartilage development positive regulation of transcription from RNA polymerase II promoter involved in cellular response to chemical stimulus Mullerian duct regression transcription, DNA-templated protein phosphorylation germ cell development erythrocyte differentiation positive regulation of transcription, DNA-templated cardiac muscle contraction cellular response to organic cyclic compound
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